12,469 results

1. TFAP4_BHLH_5-2

   Plasmid

   #185056

   Purpose

   Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   TFAP4_5_2_gRNA (Tcfap4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCW57.1-GFP_mmu-miR-16-5p_sponge

   Plasmid

   #132624

   Purpose

   Expresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTR

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-16-5p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCW57.1-GFP_mmu-miR-292b-3p_sponge

   Plasmid

   #132622

   Purpose

   Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCW57.1-RFP_mmu-miR-292b-3p_sponge

   Plasmid

   #132623

   Purpose

   Expresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

   Plasmid

   #184636

   Purpose

   Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gat1 (Slc6a1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Gat1 gene with Arginine 69 changed to Lysine, to …

   Promoter

   EF1a

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBlueScript II SK-SNAP donor for Hist1h3g

   Plasmid

   #186932

   Purpose

   Genomic targeting of SNAP tag at Hist1h3g C-terminal

   Depositor

   Zhiguo Zhang

   Article

   Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

   Insert

   SNAP donor for Hist1h3g (H3c8 Mouse)
   

   Use

   Tags

   SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. px459-Flag-Mcm2 targeting sgRNA

   Plasmid

   #186937

   Purpose

   Genomic targeting of Flag tag at Mcm2 N-terminal

   Depositor

   Zhiguo Zhang

   Article

   Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

   Insert

   Flag-Mcm2 targeting sgRNA (Mcm2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. px459-Daxx KO sgRNA

   Plasmid

   #186939

   Purpose

   Daxx KO in mouse ES cells

   Depositor

   Zhiguo Zhang

   Article

   Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

   Insert

   Daxx KO sgRNA (Daxx Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. iRFP670-KASH2ext

   Plasmid

   #187022

   Purpose

   Control for inducible disruption of LINC Complex

   Depositor

   Jan Lammerding

   Insert

   KASH2ext
   

   Use

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. px459-Mcm2-2A mutation sgRNA

    Plasmid

    #186936

    Purpose

    sgRNA used for genetic mutation at Y81 and Y90 of Mcm2

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Mcm2-2A mutation sgRNA (Mcm2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. px459-Pole4 KO sgRNA

    Plasmid

    #186935

    Purpose

    Pole4 KO in mouse ES cells

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Pole4 KO sgRNA (Pole4 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px459-Pole3 KO sgRNA

    Plasmid

    #186934

    Purpose

    Pole3 KO in mouse ES cells

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Pole3 KO sgRNA (Pole3 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine

    Plasmid

    #186418

    Purpose

    Expression of scrambled shRNA used as a control for knock down experiments

    Depositor

    Mary Kay Lobo

    Article

    Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

    Insert

    ScramblemiR
    

    Use

    AAV

    Tags

    mCitrine

    Expression

    Mutation

    Promoter

    human Synapsin1

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

    Plasmid

    #186419

    Purpose

    Expression of shRNA for the knock down of mouse Egr3

    Depositor

    Mary Kay Lobo

    Article

    Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

    Insert

    miREgr3-5D
    

    Use

    AAV

    Tags

    mCitrine

    Expression

    Mutation

    Promoter

    human Synapsin1

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pClgn1.1_Dcst1-3xHA

    Plasmid

    #183540

    Purpose

    Express Dcst1-3xHA under the mouse Clgn promoter.

    Depositor

    Masahito Ikawa

    Article

    Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

    Insert

    DC-STAMP domain containing 1 (Dcst1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

    Plasmid

    #158771

    Purpose

    Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers, ADVVCC/QMSY cleavage site

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmiRFP2-P2A-HO1-N1

    Plasmid

    #178966

    Purpose

    Expresses miRFP2 and HO1 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2-P2A-HO1
    

    Use

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-miRFP2-IRES-HO1-N1

    Plasmid

    #178967

    Purpose

    Expresses miRFP2 and HO1 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2
    

    Use

    Tags

    HO1 and no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-IRES-GFP-mouse-Caspase-1-H340D

    Plasmid

    #183362

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 H340D (Casp1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    H340D

    Promoter

    MESV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-IRES-GFP-Mouse-caspase-1/11a

    Plasmid

    #183391

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FAK Fus

    Plasmid

    #186145

    Purpose

    For expression of recombinant FAK using baculovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    C-terminal linker swapped with FUS IDR

    Promoter

    polyhedrin promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FAK C-term

    Plasmid

    #186147

    Purpose

    For expresseion of recombinant FAK c-terminal domain in E. coli

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFastBac FAK W266A

    Plasmid

    #186144

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    DNA mutation T796G;G797C Amino acid mutation W266A

    Promoter

    polyhedrin promoter

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lck

    Plasmid

    #186130

    Purpose

    For expression of recombinant Lck in SF9 cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    Lck (Lck Mouse)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    A1687T

    Promoter

    polyhedrin promoter

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FAK Pyk2

    Plasmid

    #186146

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    C-terminal linker swapped with Pyk2 linker

    Promoter

    polyhedrin promoter

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-hSyn-GFP-IRES-mSNCA(1-95)

    Plasmid

    #185716

    Purpose

    AAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (Snca Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    deleted AA 95-144

    Promoter

    hSyn

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-CMV-Cyp26a1

    Plasmid

    #185563

    Purpose

    AAV construct to ubiquitously express mouse Cyp26a1

    Depositor

    Connie Cepko

    Article

    Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

    Insert

    Cyp26a1 (Cyp26a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TRE-RARa-VP16

    Plasmid

    #185561

    Purpose

    Doxycycline inducible expression of constitutively active Retinoic Acid Receptor alpha

    Depositor

    Connie Cepko

    Article

    Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

    Insert

    RARa-VP16 (Rara Mouse)
    

    Use

    Tags

    VP16

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTriEx4_Rap1

    Plasmid

    #184662

    Purpose

    Mammalian expression of mouse Rap1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPC-FLAG.Mm.EPC1.delta.EPcA

    Plasmid

    #184655

    Purpose

    Retroviral expression of mouse EPC1 delta EPcA

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    epc1 delta EPcA

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge