We narrowed to 11,713 results for: NSI

1. pCI-neo-RL-ZEB1

   Plasmid

   #35535

   Depositor

   Greg Goodall

   Article

   Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

   Insert

   3'UTR of ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   May 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRM055 His-TEV-Cas9 6xNLS 0C

   Plasmid

   #196246

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5-25BoxB

   Plasmid

   #60817

   Purpose

   Cloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFP

   Depositor

   Maria Carmo-Fonseca

   Article

   Martin et al Cell Rep. 2013 Sep 26;4(6):1144-55. doi:

   Insert

   25x BoxB
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   25 repeats of BoxB sequence

   Promoter

   CMV

   Available Since

   Nov. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

   Plasmid

   #159281

   Purpose

   A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Insert

   AsCpf1-HA-2A-GFP
   

   Use

   Multicistronic vector with both caggs promoter-dr…

   Tags

   3X HA

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. alpha catenin conformation sensor

   Plasmid

   #71709

   Purpose

   This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   alpha-catenin FRET Sensor
   

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hsyn-GRAB_rDA-mut

   Plasmid

   #140558

   Purpose

   Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-puro-irf3-shrna1

   Plasmid

   #127648

   Purpose

   Knock-down of human IRF3

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   IRF3 shRNA (IRF3 Human)
   

   Use

   Lentiviral

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-ORF9b

   Plasmid

   #165122

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   ORF9b
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-Flag-hSox2

   Plasmid

   #20073

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   hSox2 (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHBS1449 SED1-Lenti

    Plasmid

    #180824

    Purpose

    SED1 biosensor for stable integration and expression in mammalian cells

    Depositor

    Rajat Rohatgi

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 osmosensor
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-HA-GOLPH3

    Plasmid

    #21687

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-HisMBP-SED1

    Plasmid

    #178191

    Purpose

    SED1 biosensor for expression in Escherichia coli

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-anti-GFP(LaG17) PAGER(TF)

    Plasmid

    #229997

    Purpose

    Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nef-CIAO2-Flp

    Plasmid

    #149296

    Purpose

    Cre-dependent expression of Flp recombinase.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    Flp recombinase (flp Budding Yeast)
    

    Use

    AAV

    Mutation

    Derived from the mouse codon-optomized FlpO. The…

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-FKBP-LCa

    Plasmid

    #59353

    Purpose

    Expresses human clathrin light chain A tagged with GFP & FKBP in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

    Insert

    Clathrin Light Chain A (CLTA Human)
    

    Tags

    EGFP and FKBP

    Expression

    Mammalian

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST40-2XFL-Src-Y530F

    Plasmid

    #140319

    Purpose

    Expresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Y530F

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-4493NES

    Plasmid

    #138373

    Purpose

    Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-PKA
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

    Plasmid

    #100846

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1a
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. MAC_C_AIFM1

    Plasmid

    #111659

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    AIFM1 (AIFM1 Human)
    

    Expression

    Mammalian

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FLEX-ArchT-GFP

    Plasmid

    #28307

    Purpose

    Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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