We narrowed to 8,946 results for: sgRNA

Showing: 6901 - 6920 of 8946 results

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pDGE506

Plasmid

#153279

Purpose

Intermediate sgRNA array cloning vector position 4

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE505

Plasmid

#153278

Purpose

Intermediate sgRNA array cloning vector position 3

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP1

Plasmid

#66581

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pX330.Pten.a

Plasmid

#66587

Purpose

sgRNA for Pten deletion

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

Pten deletion (Pten Mouse)

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pLV312.3

Plasmid

#119944

Purpose

Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

Plasmid

#99690

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)

Use

AAV and CRISPR

Tags

VPR mini

Mutation

dead Cas9

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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sgTP53(B)

Plasmid

#85560

Purpose

Expresses sgRNA targeting human TP53 around codon F109

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
FUG-T2A-Cas9

Plasmid

#75346

Purpose

Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

Depositor

Bryan Luikart

Article

Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

GFP (via t2a)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pdCRISPomyces-2BD

Plasmid

#234429

Purpose

Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

Depositor

Min-Kyu Oh

Article

Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

Insert

Modified dCas9(D10A, H840A) with polyaspartate linking

Expression

Bacterial

Mutation

Linked polyaspartate using glycine-serine linker

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pX330 PLIN2

Plasmid

#202196

Purpose

pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLGR002 LGR Cas9 guide vector

Plasmid

#188320

Purpose

Universal backbone for CRISPR/Cas9 sgRNA expression

Depositor

Laralynne Przybyla

Article

Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

PuroR-T2A-mTagBFP2

Expression

Mammalian

Promoter

pU6 5'CMV and EF1-alpha

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSECC-sgTom

Plasmid

#138661

Purpose

Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgTomato

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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SiC-V1-Scr

Plasmid

#133042

Purpose

SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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CSAC-Pers

Plasmid

#168281

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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CSAC-Crys

Plasmid

#164967

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2

Use

AAV

Tags

mCherry

Promoter

hU6, hSyn

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgNT

Plasmid

#138678

Purpose

Expresses a Non-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNT

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK614

Plasmid

#110608

Purpose

BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

dCas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pNSA-ARS-CRISPR-BlastR

Plasmid

#101009

Purpose

Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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LCV2 V-1

Plasmid

#84804

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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ARL13B-(Y86C)-GFP

Plasmid

#246840

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with Y86C mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Tyrosine 86 to Cysteine

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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