We narrowed to 9,102 results for: sgRNA
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Plasmid#199474PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorInsertmTagBFP2
UseCRISPRAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2
Plasmid#199483PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorInsertGal4vp16/4xnrUAS-mKate2
UseCRISPRAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHpgRNA13
Plasmid#160127PurposeTo express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1DepositorInsertPTEF1-HH-gRNA-ADE2- HDV-TAMO
ExpressionYeastAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry
Plasmid#170182PurposeMammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry
Tags3XFlag and T2AExpressionMammalianPromoterCBhAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
px330 SUZ12 - pDY083
Plasmid#171109PurposeExpresses Cas9 and an sgRNA targeting SUZ12DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
ExpressionMammalianAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHX_gRNA_hPAX7_6-9S
Plasmid#160453PurposesgRNA targeting human PAX7 3' UTR with 17bp spacerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_hPAX7_6-9S
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
px330 EQR
Plasmid#101733PurposeExpresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifsDepositorInsertSpCas9 EQR
Tags3xFLAG-NLS and NLSExpressionMammalianMutationEQR (D1135E, R1335Q, and T1337R mutations in Cas9)Available sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330.iGFP1
Plasmid#66581PurposesgRNA for iGFPDepositorPublicationInsertiGFP
ExpressionMammalianAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330.Pten.a
Plasmid#66587PurposesgRNA for Pten deletionDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPten deletion (Pten Mouse)
ExpressionMammalianAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP1354
Plasmid#119275Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-moxGFP-GOLGA2
Plasmid#207792PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-moxGFP-LMNB1
Plasmid#207773PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pGES201
Plasmid#190197PurposeFor CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selectionDepositorInsertspCas9
UseCRISPRTags3xFlagExpressionPlantMutationplant-codon optimizedPromoterGlycine max elongation factor 1A(pM4)Available sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSI-356 v2
Plasmid#158431PurposeSpCas9 sgRNA cloning backboneDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterHuman H6Available sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT56
Plasmid#223428PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_Puro_sgAAVS1
Plasmid#187818PurposepLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1DepositorInsertsgAAVS1
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb
Plasmid#99692PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAVDepositorPublicationAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Donor/Reporter-COL4A3
Plasmid#130279PurposeCOL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor templateDepositorInsertmCherry-eGFP
UseAAVPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LRT2B-GO
Plasmid#136908PurposeLentivirus expressing GO sgRNADepositorInsertsgGO
UseLentiviralMutationWTPromoterhU6Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only