We narrowed to 7,117 results for: 11

1. DNA-PKcs T3950D

   Plasmid

   #83320

   Purpose

   DNA-PKcs T3950A

   Depositor

   Katheryn Meek

   Article

   Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Activation loop phosphorylation site 3950 substit…

   Promoter

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Puro-mGSDME

   Plasmid

   #154877

   Purpose

   Expresses mGSDME in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (Gsdme Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pExp-xMBP-ACE2-CHis

   Plasmid

   #194998

   Purpose

   Production of ACE2 extracellular domain

   Depositor

   Marko Hyvönen

   Article

   Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Tags

   His8 and xMBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETcon_SARS-CoV-2_RBD

   Plasmid

   #166782

   Purpose

   Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

   Depositor

   Jesse Bloom

   Article

   Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

   Insert

   Spike receptor binding domain (RBD) from SARS-CoV-2 (S )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcsII-LMP7-flag

   Plasmid

   #86773

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   immunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. hyeA3A-BE4max

   Plasmid

   #157944

   Purpose

   Mammalian expression of hyeA3A-BE4max

   Depositor

   Dali Li

   Article

   Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

   Insert

   hyeA3A-BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

   Plasmid

   #86457

   Purpose

   Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

   Depositor

   Ralf Kuehn

   Article

   Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   BFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pgk

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-TetON-GFP

   Plasmid

   #115495

   Purpose

   Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGLS3-5xHRE-p53(K120R)

   Plasmid

   #72554

   Purpose

   Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.

   Depositor

   Ester Hammond

   Article

   Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   5XHRE

   Expression

   Mammalian

   Mutation

   K120R

   Promoter

   E1B minimal promoter

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT Smarca4-V5 IRES-puro

    Plasmid

    #107058

    Purpose

    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

    Depositor

    Jerry Crabtree, Courtney Hodges

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-puroR and V5

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CAG

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSAM2_mCherry_Tbx20

    Plasmid

    #72697

    Purpose

    Doxycyline regulated expression of Tbx20 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Tbx20 (Tbx20 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. hyA3A-BE4max

    Plasmid

    #157943

    Purpose

    Mammalian expression of hyA3A-BE4max

    Depositor

    Dali Li

    Article

    Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

    Insert

    hyA3A-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid

    #50486

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    GFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the GFP open…

    Promoter

    CMV

    Available since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSAM2_mCherry_Baf60c

    Plasmid

    #72691

    Purpose

    Doxycyline regulated expression of Baf60c in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Brg 1 (SMARCD3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. K785R Smarca4-sfGFP

    Plasmid

    #107057

    Purpose

    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

    Depositor

    Jerry Crabtree, Courtney Hodges

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    Super-folder GFP (sfGFP)

    Expression

    Mammalian

    Mutation

    K785R

    Promoter

    CAG

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJS647

    Plasmid

    #183176

    Purpose

    PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Insert

    PYR1 (PYR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCRISPR v2 puro hGSDME gRNA1

    Plasmid

    #223519

    Purpose

    knocking out hGSDME in human cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-puro-mGSDME D270A

    Plasmid

    #223518

    Purpose

    Expresses mGSDME D270A in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (Gsdme Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D270A

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hyBE4max

    Plasmid

    #157942

    Purpose

    Mammalian expression of hyBE4max

    Depositor

    Dali Li

    Article

    Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

    Insert

    hyBE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX6P1-3xMBT_D355N

    Plasmid

    #46988

    Purpose

    Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli

    Depositor

    Or Gozani

    Article

    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

    Insert

    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    

    Use

    Tags

    GST and PreScission cleavage site

    Expression

    Bacterial

    Mutation

    Mutation aspartic acid 355 to asparagine (D355N);…

    Promoter

    tac

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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