We narrowed to 15,731 results for: LEA

1. pHR-HP1α-miRFP670-Cry2WT

   Plasmid

   #122442

   Purpose

   Expresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   HP1 alpha (CBX5 Human)
   

   Use

   Lentiviral

   Tags

   Cry2WT and miRFP670

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ230-RR

   Plasmid

   #108860

   Purpose

   LbCpf1 Gateway entry plasmid with G548R and K611R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G548R and K611R, Cpf1 is rice codon optimized

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDipA-TEV-His12

   Plasmid

   #137042

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
   

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. OCT4 GFP puro Donor 1

   Plasmid

   #22209

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   SA-OCT-GFP-2APuro-PA (POU5F1 Human)
   

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMVA2

   Plasmid

   #119951

   Purpose

   Expresses recombinant MVA pathway in Escherichia coli

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   mvaE
   mvaS
   mvaK1
   mvaK2
   mvaD
   idi
   

   Expression

   Bacterial

   Mutation

   A110G

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-TetO-Tead4

   Plasmid

   #128832

   Purpose

   dox-dependent expression of mouse Tead4

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

   Insert

   Tead4 (Tead4 Mouse)
   

   Use

   Lentiviral

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiU6-LacZ-GFP-Puro (BB)

   Plasmid

   #170459

   Purpose

   For cloning gRNA block

   Depositor

   Yonglun Luo

   Article

   Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

   Insert

   hU6-BB-LacZ and EF1A-EGFP-2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6 for gRNA and EF1A for EGFP-2A-Puro

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUMO-β′-COPI-WD40

   Plasmid

   #180753

   Purpose

   Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

   Insert

   β′-COPI-WD40 (SEC27 Budding Yeast)
   

   Tags

   N-terminal strep tag, His-tag, and SUMO tag

   Expression

   Bacterial

   Mutation

   Encodes residues 1-301; deleted 302-889; mutated …

   Promoter

   T7

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

   Plasmid

   #133044

   Purpose

   Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

   Insert

   Xph20-ETWV
   

   Tags

   NLS, TEV protease + TEV cleavage site, and miRFP6…

   Expression

   Mammalian

   Mutation

   S63K

   Promoter

   CAG

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-TEV-U1AF37MF77M

    Plasmid

    #168267

    Purpose

    Expresses human dmU1A with the F37M/F77M double mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    U1A RRM1 crystallization module (SNRPA Human)
    

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F37M and F77M

    Promoter

    T7 promotor

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TVBB C-term-mTurquoise2

    Plasmid

    #169223

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NRCH-APOBEC-nCas9-Ung

    Plasmid

    #198552

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-PKHD1-myc

    Plasmid

    #108407

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

    Depositor

    Gregory Germino

    Article

    Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

    Insert

    PKHD1 (PKHD1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

    Plasmid

    #163572

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT-Flag-NRBP1

    Plasmid

    #48197

    Purpose

    Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

    Insert

    NRBP1 (NRBP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pGBT9-Hook3

    Plasmid

    #198525

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook3 (HOOK3 Human)
    

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Met1 diUb (1-152)

    Plasmid

    #110766

    Purpose

    Bacterial expression of Met1 diUb

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    UBB (UBB Human)
    

    Tags

    N-His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-GFP-PKHD1

    Plasmid

    #108408

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

    Depositor

    Gregory Germino

    Article

    Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

    Insert

    PKHD1 (PKHD1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIG-LRRC37Bchimpanzee-HA

    Plasmid

    #220794

    Purpose

    To express the chimpanzee receptor LRRC37B in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    LRRC37B
    

    Tags

    HA

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR201-Eps8L2

    Plasmid

    #196526

    Purpose

    Entry vector for Gateway with Eps8L2

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2-FL (EPS8L2 Human)
    

    Expression

    Bacterial

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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