We narrowed to 15,601 results for: LEA

1. pCWXPGR-pTF-DVL2deltaDIX

   Plasmid

   #114276

   Purpose

   TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   DVL2 (Dvl2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of DIX domain

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay-GRAB_CRF0.5

   Plasmid

   #208654

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJWK_VD_01

   Plasmid

   #158102

   Purpose

   5'UTR stem-loop luciferase reporter: 0kcal/mol

   Depositor

   Ted Dawson

   Article

   Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

   Insert

   5'UTR synthetic stem-loop 0kcal/mol
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. OCT4 GFP puro Donor 3

   Plasmid

   #22211

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   SA-OCT-GFP-2APuro-PA (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO_NWS.Ollas.S_mCherry-NLS

   Plasmid

   #178279

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   NWS.Ollas.S and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO_HSV.S.V5_mCherry-NLS

   Plasmid

   #178278

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.S.V5 and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO_HSV.Ollas.V5_mCherry-NLS

   Plasmid

   #178277

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.Ollas.V5 and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_HSV.NWS.S_mCherry-NLS

   Plasmid

   #178273

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.NWS.S and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-SpCas9-HF1-plus-NLS-6xHis

   Plasmid

   #207381

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, Q695A, Q926A, amino acids 1005-1013 replac…

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-B-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207385

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_DIO_R133C-HaloTag

    Plasmid

    #164064

    Purpose

    Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

    Depositor

    Nathaniel Heintz

    Article

    Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

    Insert

    MeCP2 Isoform 1 R133C (Mecp2 Mouse)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ239-RR

    Plasmid

    #108862

    Purpose

    FnCpf1 Gateway entry plasmid with N623R and K687R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N623R and K687R, Cpf1 is rice codon optimized

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Ctdnep1_D67E-2xFlag

    Plasmid

    #196519

    Purpose

    Expression of Ctdnep1_D67E-2xFlag

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1_D67E-2xFlag (CTDNEP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    D67E mutation (phosphastase dead)

    Promoter

    CMV promoter; T7 promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1+CeGFP-Ctdnep1WTsiR

    Plasmid

    #196517

    Purpose

    Expression of Ctdnep1WT-GFP

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1WTsiR (CTDNEP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Silent mutations for siRNA resistance

    Promoter

    CMV promoter; T7 promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-LC3-K51A

    Plasmid

    #155265

    Purpose

    Mammalian expression of rat LC3 fused to EGFP

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    Lysine 51 to Alanine

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-LC3-R70A

    Plasmid

    #155264

    Purpose

    Mammalian expression of rat LC3 fused to EGFP

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    Arginine 70 to Alanine

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

    Plasmid

    #163571

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Rat Alpha2 delta-1 mutMIDAS pMT2

    Plasmid

    #58730

    Purpose

    expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi

    Insert

    alpha2 delta-1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MIDAS site mutations (D259VSGS to AVAGA)

    Promoter

    SV40

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Drosha1-850

    Plasmid

    #62522

    Purpose

    C-terminal aa851-1374 of Drosha is deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with aa 851-1374 deleted (DROSHA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 851-1374

    Promoter

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

20. HOXB3 (human) FLAG pSP65

    Plasmid

    #8523

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only