We narrowed to 15,882 results for: LEA

Showing: 6941 - 6960 of 15882 results

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT

Plasmid

#187585

Purpose

Express FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NPAT (NPAT Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2 V-3

Plasmid

#84806

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

Plasmid

#187574

Purpose

Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

RNPS1 (RNPS1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_CRFmut

Plasmid

#208656

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#214052

Purpose

Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Target sequence

Use

Target rna plasmid

Expression

Bacterial

Promoter

trc promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHochTypeIII

Plasmid

#214039

Purpose

Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Cas10

Use

CRISPR

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

Plasmid

#187579

Purpose

Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

PSPC1 (PSPC1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-T7-EcKlenow (LM2692)

Plasmid

#208963

Purpose

Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

EcKlenow-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shSTING-Mmu

Plasmid

#127697

Purpose

Doxycyclin inducible shRNA knockdown of mouse STING gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shSTING Mmu (Sting1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-C (TRP1)

Plasmid

#177795

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet

Plasmid

#169219

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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7TFP CDH1 mutant reporter

Plasmid

#91703

Purpose

lentiviral luciferase reporter containing CDH1 promoter with mutant E-boxes

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

CDH1 mutant promoter (CDH1 Human)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutant E-boxes

Promoter

CDH1 mutant

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

Plasmid

#187584

Purpose

Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SP100 (SP100 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB N-term-mTurquoise2

Plasmid

#169222

Purpose

Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pExodus CMV.Artemis

Plasmid

#40211

Purpose

Mammalian expression of codon-optimized Artemis.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Artemis (DCLRE1C Human)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6-150

Plasmid

#91777

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-150

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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HS-GFP-PACT

Plasmid

#105954

Purpose

heat shock inducible transgenesis, PACT is a centriol marker

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

PACT (AKAP9 Human)

Tags

GFP

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDsRed-importinα

Plasmid

#119679

Purpose

Expresses DsRed-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

importinα (KPNA2 Human)

Tags

DsRed

Expression

Mammalian

Mutation

Contains amino acids 251-529

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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OCT4-eGFP-2A-Puro

Plasmid

#31939

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Inserts

OCT4 (POU5F1 Human)
eGFP (eGFP )

Use

Targeting donor

Tags

eGFP

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-SpyTag-Flag-SRSF3-V5-mCherry

Plasmid

#235091

Purpose

SRSF3 mCherry fusion protein without MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-SpyTag-Flag-SRSF3-V5-mCherry (SRSF3 Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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