We narrowed to 15,731 results for: LEA

1. TVBB N-term-mNeongreen

   Plasmid

   #169226

   Purpose

   Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBA57-TEV-His12

   Plasmid

   #137033

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
   

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length BBA57, contains signal pep…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV Gpat4-V5

   Plasmid

   #175145

   Purpose

   Lentiviral expression of mouse Gpat4-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Gpat4 (Gpat4 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGPN-ACE2

   Plasmid

   #187483

   Purpose

   Expression vector - ACE2

   Depositor

   Lewis Cantley

   Article

   Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Promoter

   EFS

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ230-RVR

   Plasmid

   #108861

   Purpose

   LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G548R, K554V and Y558R, Cpf1 is rice codon optimi…

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

   Plasmid

   #163545

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSAVED-CHAT_PCaspase_PCi_PC-σ

   Plasmid

   #214040

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-Pci-PC-σ
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Polymerase expression - pCMV-ePhi29 (LM2802)

   Plasmid

   #208967

   Purpose

   Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PITX3-2A-eGFP-PGK-Puro

   Plasmid

   #31943

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   PITX3 (PITX3 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Promoter

   -

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2-CMV/TetO2-EYFP-GW

    Plasmid

    #153309

    Purpose

    Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Yellow Fluorescent Protein

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYFP-ErbB2ΔC990

    Plasmid

    #66946

    Purpose

    Mammalian expression of rat ErbB2 ΔC990 tagged with YFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    Deletion of C990. (see depositor comment below on…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pLKO_AU1.HSV.S_mCherry-NLS

    Plasmid

    #178224

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HSV.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYFP-ErbB2ΔC776

    Plasmid

    #66947

    Purpose

    Mammalian expression of rat ErbB2 ΔC776 tagged with YFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    Deletion of C776 (see depositor comment below on …

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pDTFendoNA2

    Plasmid

    #174731

    Purpose

    Expresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.

    Depositor

    Jukka Finne

    Article

    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

    Insert

    DT386F
    

    Expression

    Bacterial

    Mutation

    Deleted amino acids 412-560 (the receptor binding…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-Cas9-VQR-Blast

    Plasmid

    #87155

    Purpose

    lentiviral expression of SpCas9-VQR (NGA PAM restricted)

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    SpCas9-VQR(D1135V,R1335Q,T1337R)
    

    Use

    Lentiviral

    Mutation

    D1135V,R1335Q,T1337R

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid

    #208969

    Purpose

    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV Flag dPDZ-PTEN (1-400)

    Plasmid

    #22232

    Depositor

    Hong Wu

    Article

    Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

    Insert

    PTEN (Pten Mouse)
    

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    PDZ binding domain deleted. Contains amino acids …

    Available Since

    March 19, 2010

    Availability

    Academic Institutions and Nonprofits only

18. MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

    Plasmid

    #163719

    Purpose

    Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG
    

    Expression

    Bacterial

    Mutation

    dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

    Plasmid

    #146392

    Purpose

    Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmeIF4G-trunc (eIF4G1 Fly)
    

    Expression

    Insect

    Mutation

    322-1666 truncated version of elF4G sequence with…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_NOPLight-ctr

    Plasmid

    #195579

    Purpose

    Expresses the control sensor NOPLight-ctr in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

    Insert

    NOPLight-ctr
    

    Expression

    Mammalian

    Mutation

    D110A, D130A

    Promoter

    CMV

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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