We narrowed to 58,550 results for: tra

1. pTH862-CEN-slowmaxRLuc/minCFLuc

   Plasmid

   #115371

   Purpose

   Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase

   Depositor

   Tobias von der Haar

   Inserts

   Firefly Luciferase (codon minimised)
   Gcn4 5'-UTR + Renilla luciferase (codon optimised)
   

   Expression

   Yeast

   Mutation

   All codons have been changed for the most favoura…

   Promoter

   ADH1 and TDH3

   Available Since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-4T-3-cytXlATL2

   Plasmid

   #124063

   Purpose

   Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

   Depositor

   Tom Rapoport

   Article

   Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

   Insert

   cytXIATL2
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. N229A Lo-MetQ

   Plasmid

   #118268

   Purpose

   N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Insert

   MetQ (N229A) (metQ E. coli)
   

   Tags

   10x His Tag and Enterokinase Cleavage Site

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala)

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. zebrafish SLC2A2 (zfSLC2A2)

   Plasmid

   #65103

   Purpose

   Full length SLC2A2 cDNA (Danio rerio)

   Depositor

   Josep Planas

   Article

   Castillo et al Am J Physiol Regul Integr Comp Physiol.

   Insert

   SLC2A2 (slc2a2 Zebrafish)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

5. gRNA[bTub+DsxF].1046A

   Plasmid

   #112694

   Purpose

   express two gRNA targeting bTub & DsxF under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   U6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)
   

   Use

   CRISPR

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-Fos S362DS374A

   Plasmid

   #118305

   Purpose

   Expression of mous C-Fos

   Depositor

   Philip Stork

   Article

   Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

   Insert

   C-Fos S362DS374A (Fos Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S362DS374A

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-Fos S362AS374D

   Plasmid

   #118304

   Purpose

   Expression of mouse C-Fos

   Depositor

   Philip Stork

   Article

   Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

   Insert

   C-Fos S362AS374D (Fos Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S362AS374D

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-Fos S362D

   Plasmid

   #118303

   Purpose

   Expression of mouse C-Fos

   Depositor

   Philip Stork

   Article

   Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

   Insert

   C-Fos S362D (Fos Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S362D

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_6HT-TBP6.7(P46A)

    Plasmid

    #112721

    Purpose

    This mutation (P46A) marks the first residue within the evolved region of U1A.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P46A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P46A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a_6HT-TBP6.7(Q48T)

    Plasmid

    #112725

    Purpose

    This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48T)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48T

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a_6HT-TBP6.7(P51A)

    Plasmid

    #112728

    Purpose

    Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P51A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P51A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a_6HT-TBP6.7(Q54A)

    Plasmid

    #112730

    Purpose

    Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q54A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q54A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET29b-PD-H166Q

    Plasmid

    #110179

    Purpose

    Expresses propanediol dehydratase mutant H166Q in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    Propanediol Dehydratase
    

    Tags

    His6 and S-tag

    Expression

    Bacterial

    Mutation

    H166Q mutant

    Promoter

    T7

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET29b-PD-E440Q

    Plasmid

    #110178

    Purpose

    Expresses propanediol dehydratase mutant E440Q in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    Propanediol Dehydratase
    

    Tags

    His6 and S-tag

    Expression

    Bacterial

    Mutation

    E440Q mutant

    Promoter

    T7

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET29b-PD-C438S

    Plasmid

    #110177

    Purpose

    Expresses propanediol dehydratase mutant C438S in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    Propanediol Dehydratase
    

    Tags

    His6 and S-tag

    Expression

    Bacterial

    Mutation

    C438S mutant

    Promoter

    T7

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET29b-PD-G817A

    Plasmid

    #110174

    Purpose

    Expresses propanediol dehydratase mutant G817A in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    Propanediol Dehydratase
    

    Tags

    His6 and S-tag

    Expression

    Bacterial

    Mutation

    G817A mutant

    Promoter

    T7

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

    Plasmid

    #107739

    Purpose

    mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein(C1A)
    

    Expression

    Worm

    Mutation

    change the first cysteine of gp41-1 N-intein to a…

    Promoter

    C. elegans myo-2 promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AY23_pgRNA.R5.2

    Plasmid

    #100295

    Purpose

    CCR5-targeting gRNA expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6 Polymerase III snRNA

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CFFΔC-EGFP

    Plasmid

    #86059

    Purpose

    A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains CD8a luminal and transmembrane domains a…

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge