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We narrowed to 6,296 results for: pcDNA 3.1

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Showing: 1701 - 1750 of 6296 results

  5. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid
    #112713
    Purpose
    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Tags
    BirA* (BirA R118G), HIV Rev nuclear localization …
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    112713-plasmid-map-sequence-id-217067

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  9. pcDNA3.1-mouse cdk6 R31C

    Plasmid
    #75171
    Purpose
    Mammalian expression of mouse cdk6 with R31C
    Depositor
    Martine Roussel
    Insert
    cyclin dependent kinase 6 (Cdk6 Mouse)
    Expression
    Mammalian
    Mutation
    R31C
    Promoter
    CMV
    Available Since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA3.1-hRIPK1-Flag-K599R

    Plasmid
    #112490
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K599R
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112490-plasmid-map-sequence-id-214537

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  16. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. Mouse NCKX2-AS-long (pcDNA3.1+)

    Plasmid
    #75199
    Purpose
    Mammalian expression of SLC24A2
    Depositor
    Jonathan Lytton
    Insert
    SLC24A2 (Slc24a2 Mouse)
    Expression
    Mammalian
    Mutation
    alternatively spliced exon present
    Promoter
    CMV
    Available Since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75199-plasmid-map-sequence-id-142841

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  18. pcDNA3.1-hRIPK1-Flag-K115R

    Plasmid
    #112488
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K115R
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112488-plasmid-map-sequence-id-214538

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  28. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pcDNA3.1-Gαi1-Gβ1-Gγ2

    Plasmid
    #254720
    Purpose
    Expresses G protein heterotrimer with Gαi1, Gβ1 and Gγ2 ('dark'). Vector used as control for G protein recruitment or 'nuc-BRET' assay. Based on TRUPATH Triple Gαi1 (#196048).
    Depositor
    Cornelius Gati
    Article
    Khan et al Nature. 2025 Dec;648(8094):755-763. doi:
    Inserts
    Gαi1 (GNAI1 Human)
    Gβ1 (GNB1 Human)
    Gγ2 (GNG2 Human)
    Expression
    Mammalian
    Promoter
    CMV, IRES, and T2A
    Available Since
    May 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    254720-plasmid-map-sequence-id-509987

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  31. pcDNA3.1-Gαi1ΔF-Gβ1-Gγ2

    Plasmid
    #254721
    Purpose
    Expresses G protein heterotrimer with Gαi1-ΔF, Gβ1 and Gγ2-GFP2 fusion as negative control. Vector used as controlfor G protein recruitment or 'nuc-BRET' assay. Based on TRUPATH Triple Gαi1 (#196048).
    Depositor
    Cornelius Gati
    Article
    Khan et al Nature. 2025 Dec;648(8094):755-763. doi:
    Inserts
    Gαi1 (GNAI1 Human)
    Gβ1 (GNB1 Human)
    Gγ2 (GNG2 Human)
    Tags
    GFP2
    Expression
    Mammalian
    Mutation
    deleted C-terminal amino acid F264
    Promoter
    CMV, IRES, and T2A
    Available Since
    May 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    254721-plasmid-map-sequence-id-509983

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  34. pcDNA3.1-Gαz Q205L-YFP

    Plasmid
    #250702
    Purpose
    Expressing Citrine version of YFP fused human Gαz Q205L
    Depositor
    Mikel Garcia-Marcos
    Insert
    G_z Q205L-YFP
    Expression
    Mammalian
    Mutation
    Q205L in G_z sequence
    Promoter
    CMV
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    250702-plasmid-map-sequence-id-507426

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  35. pcDNA3.1-Gαs R201C (short)

    Plasmid
    #250708
    Purpose
    Expressing human Gαs R201C mutant, short isoform
    Depositor
    Mikel Garcia-Marcos
    Insert
    G_s R201C (short isoform)
    Expression
    Mammalian
    Mutation
    R201C in G_s sequence
    Promoter
    CMV
    Available Since
    March 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250708-plasmid-map-sequence-id-501629

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  36. pcDNA3.1-Gαs S275L (short)

    Plasmid
    #250710
    Purpose
    Expressing human Gαs S275L mutant, short isoform
    Depositor
    Mikel Garcia-Marcos
    Insert
    G_s S275L (short isoform)
    Expression
    Mammalian
    Mutation
    S275L in G_s sequence
    Promoter
    CMV
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    250710-plasmid-map-sequence-id-501490

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  37. pcDNA3.1-Gαs Q227L (short)

    Plasmid
    #250709
    Purpose
    Expressing human Gαs Q227L mutant, short isoform
    Depositor
    Mikel Garcia-Marcos
    Insert
    G_s Q227L (short isoform)
    Expression
    Mammalian
    Mutation
    Q227L in G_s sequence
    Promoter
    CMV
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    250709-plasmid-map-sequence-id-501481

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  47. pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-HA

    Plasmid
    #239766
    Purpose
    Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060
    Insert
    Scn8a (Scn8a Mouse)
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1425TAG, Y371C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239766-plasmid-map-sequence-id-477737

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  48. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C)-HA

    Plasmid
    #239767
    Purpose
    Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060
    Insert
    Scn8a (Scn8a Mouse)
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1546TAG, Y371C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239767-plasmid-map-sequence-id-477107

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  50. pcDNA3.1-IARS2-H112A/P115A

    Plasmid
    #232338
    Purpose
    Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Expression
    Mammalian
    Mutation
    Putative isoleucine binding deficiency mutation H…
    Promoter
    CMV
    Available Since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232338-plasmid-map-sequence-id-474537

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Showing: 1701 - 1750 of 6296 results