We narrowed to 7,067 results for: FIE
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Plasmid#146577PurposeInsect Expression of DmeIF4GaDepositorInsertDmeIF4Ga (eIF4G1 Fly)
ExpressionInsectMutationone non silent mutation K340R compared to the seq…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT22gCfpUbiG4FER4P2A(#249)
Plasmid#184070Purposecyclofen-inducible GAL4 activation in zebrafish permanent transgenicDepositorInsertGal4bdFF-ERT2-P2A
UseZebrafish transgenesis (blue eyes)MutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin
Plasmid#182305PurposeE. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.DepositorInsertpH-Countdown insertion into GECO 1.2 calcium sensing domains
TagsA modified TorA periplasmic targeting sequence pl…ExpressionBacterialMutationSpontaneous mutation of calmodulin's penulti…Promotert7Available SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q03JI6
Plasmid#103123PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ03JI6 (Cas9 coding gene from Campylobacter jejuni)
UseCRISPR; Cloning vectorTagsSV40NLSMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_VPEVS
Plasmid#105863PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
ExpressionMammalianMutationmutated heavy chain of mouse IgG3 with five mutat…PromoterhEF1-HTLV promAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_ILGGP
Plasmid#105855PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
ExpressionMammalianMutationmutated heavy chain of mouse IgG1 with five mutat…PromoterhEF1-HTLV promAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_CH2charge
Plasmid#105852PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutations modify charge of CH2 domain.DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
ExpressionMammalianMutationmutated heavy chain of mouse IgG1, muatations: Gl…PromoterhEF1-HTLV promAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSP64T3 BMP2 pro dnmVg1 (DM#182)
Plasmid#15074DepositorInsertBMP2-Vg1 dom neg
UseSp6 expression for use in xenopusMutationdominant negative version of BMP2-Vg1 chimera. Th…Available SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GP-TFIIB(D207A)-2xStrep
Plasmid#213920PurposeSleeping beauty vector for expression of C-terminally strep-tagged TFIIB(D207A)DepositorInsertTFIIB(D207A)-2xStrep (GTF2B Human)
Tags2xStrepExpressionMammalianMutation(1) D207A (2) Coding region changed for resistanc…PromoterEF-1EF1a/RPBSAAvailable SinceJune 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GP-TFIIB(S65A)-2xStrep
Plasmid#213921PurposeSleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65A)DepositorInsertTFIIB(S65A)-2xStrep (GTF2B Human)
Tags2xStrepExpressionMammalianMutation(1) S65A (2) Coding region changed for resistance…PromoterEF-1EF1a/RPBSAAvailable SinceJune 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GP-TFIIB(S65E)-2xStrep
Plasmid#213922PurposeSleeping beauty vector for expression of C-terminally strep-tagged TFIIB(S65E)DepositorInsertTFIIB(S65E)-2xStrep (GTF2B Human)
Tags2xStrepExpressionMammalianMutation(1) S65E (2) Coding region changed for resistance…PromoterEF-1EF1a/RPBSAAvailable SinceJune 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/MALAT1[C8351G] (pAVA3169)
Plasmid#239346PurposeExpresses full-length, driven by its own promoter MALAT1 with stability-compromised ENE(C8351G) and an 11-nt insertion (cgctcgacgta).DepositorInsert10,843 bp of MALAT1 of genomic locus amplified from genomic DNA of Hela cells (MALAT1 Human)
ExpressionMammalianMutationAn 11-nt insertion (cgctcgacgta) and stability-co…Available SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)
Plasmid#223166Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG NBT:NRGIIIa_R551Q:polyA
Plasmid#193013PurposeTol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neuronsDepositorInsertsUseTransposon transgenesisExpressionMammalianMutationENST00000287842, NM_013956, c.1652G>A, p.(Arg5…Available SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22U6CatalE4PCh(#767)
Plasmid#184078Purposecyclofen-inducible Catalase activation in zebrafish permanent transgenicDepositorInsertCatalDeltaC-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes) + in vitro tra…MutationCatalDeltaC: deleted 516-527; ERT2: G400V, M543A,…PromoterZf-Ubi+SP6Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(1-720aa)
Plasmid#139687PurposeExpresses N-teminal Flag tagged aa 1- 720 of LARS1DepositorAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA8-Flag-LARS (361-720aa)
Plasmid#139688PurposeExpresses N-teminal Flag tagged aa 361-720 of LARS1DepositorAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
UASp-deltaN cycB
Plasmid#112967PurposeP element vector for transposon insertion of nondegradable Cyclin B into Drosophila genomeDepositorInsertcycB (CycB Fly)
UseP insertion vectorMutationN-term deletion of first 46 amino acids, includes…Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRE-RXRa-Myc; pGK-H2B-RFP
Plasmid#242887PurposeFor lentiviral overexpression of Myc-tagged human RXRa coupled to H2B-RFP (in the opposite direction)DepositorInsertRxra (RXRA Human)
UseLentiviralTagsH2B-mRFPExpressionMammalianMutationHuman RXRa cDNA was PCR-amplified from pSV-Sport-…PromoterTetracycline response element upstream of a minim…Available SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003(K31TAG)
Plasmid#210023PurposeExpression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cellsDepositorInsertEGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
ExpressionMammalianMutationSpyCatcher003 with K31TAG (Amber stop codon) muta…PromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only