165,637 results

1. pHTNW

   Plasmid

   #136403

   Purpose

   Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57.1 N-term GFP tTA

   Plasmid

   #107551

   Purpose

   All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

   Depositor

   David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP (optional)

   Expression

   Mammalian

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-UTX-F

   Plasmid

   #17438

   Purpose

   Expression of UTX in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   UTX (KDM6A Human)
   

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM5-WT PHGDH

   Plasmid

   #83901

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMLV-CAG-SADB19_oG-IRES-Puro

   Plasmid

   #172367

   Purpose

   Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   oG + Puro
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. NXPE1_pcDNA3.4-TOPO

   Plasmid

   #226841

   Purpose

   Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.

   Depositor

   Bert Vogelstein

   Insert

   NXPE1 (NXPE1 Human)
   

   Tags

   x6 HIS and an IL2 secretion signal both N termina…

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G

   Plasmid

   #194464

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEFBOS_CreIRESpuro

   Plasmid

   #183812

   Purpose

   Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

   Depositor

   Ed Stanley

   Article

   Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

   Insert

   Cre recombinase
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. DsRed2-N1

    Plasmid

    #54493

    Purpose

    Localization: N1 Cloning Vector, Excitation: 558, Emission: 583

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    DsRed2

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCDNA3-deltaBamHI-human MIZ1

    Plasmid

    #74167

    Purpose

    Mammalian expression of human MIZ1

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MIZ1 (ZBTB17 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3 NQO1

    Plasmid

    #61730

    Purpose

    Expresses WT human NQO1

    Depositor

    Yosef Shaul

    Article

    Adamovich et al Mol Cell Biol. 2013 Jul;33(13):2603-13.

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEGFP-WT-STAT3

    Plasmid

    #71450

    Purpose

    Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. B52 (empty plasmid backbone to express 2 sgRNAs)

    Plasmid

    #100708

    Purpose

    Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9-CBE6b

    Plasmid

    #215820

    Purpose

    Express CBE6b (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b-SpCas9-2xUGI (cas9 )
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE-UBC-mCD47-T2A-GFP

    Plasmid

    #205449

    Purpose

    lentiviral plasmid for expression of mouse CD47

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCD47 (Cd47 Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

    Plasmid

    #100835

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSMART Tmem192-3X HA (targeting vector for genomic tagging)

    Plasmid

    #175777

    Purpose

    pSMART Tmem192-3X HA (targeting vector for genomic tagging)

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLBH531_MBP-Cas14a1 expression

    Plasmid

    #112500

    Purpose

    Expresses MBP fused to Cas14a1

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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