159,530 results
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AA0250-mCherry-2a-hM4D-nrxn1a rev
Plasmid#60544PurposeCo-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virusDepositorInsertsUseAAV and Cre/LoxTags2xHAExpressionMutationPromoterCAGAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas3
Plasmid#209427PurposePlasmid for CASCADE-Cas3 based genome engineering of streptomycetesDepositorInsertcodon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; crRNA under control of PermE*Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN mEGFP
Plasmid#188643PurposeThis is the Lazarou lab's standard retrovirus vector backbone for expression of mEGFP-tag fusions onto the N-terminus of cloned genesDepositorTypeEmpty backboneUseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-PheRS
Plasmid#124116PurposeBacterial expression plasmid for PURE system componentsDepositorUseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0885
Plasmid#142210PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPPARG (PPARG Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget
Plasmid#214052PurposeExpresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.DepositorInsertTarget sequence
UseTarget rna plasmidTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNon-target
Plasmid#214053PurposeExpresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.DepositorInsertNon-target sequence
UseNon-target rna plasmidTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHochTypeIII
Plasmid#214039PurposeBacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6DepositorInsertCas10
UseCRISPRTagsExpressionMutationWTPromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorUseTagsExpressionMammalianMutationPromoterMammalian Pol IAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
R5 EMCV
Plasmid#51733PurposeDicistronic vector with EMCV IRESDepositorInsertEMCV IRES
UseTagsExpressionMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Kv-ArcLight-ST
Plasmid#182319PurposeSoma-targeted fluorescent voltage indicator with left-shifted voltage-dependencyDepositorInsertKv-ArcLight-ST
UseTagsExpressionMammalianMutationMutation in an amino acid Q217E of ArcLight-MTPromoterCAGAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IDO2-p2a-eGFP
Plasmid#187027PurposeExpresses IDO2 and GFP (seperately) in mammalian cellsDepositorInsertIndoleamine 2,3-dioxygenase 2 (IDO2 Human)
UseTagsP2A-GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-tagged Bcr
Plasmid#38189DepositorInsertBCR (BCR Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FuG-B2
Plasmid#67513PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.DepositorInsertFusion Glycoprotein type B2
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…ExpressionMutationPromoterCAGGSAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV2 Dvl-2-Myc
Plasmid#42194DepositorInsertDvl 2 (Dvl2 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-HLA-E SCT-T2A-GFP
Plasmid#205461Purposelentiviral plasmid for expression of HLA-E SCTDepositorInsertHLA-E SCT (HLA-E Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]
Plasmid#159796PurposeHistamine based negative selection marker to paralyze animals with arraysDepositorInsertpEXP[Prpl-3 | histamine | rpl-3 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-sgH11-eCas9-T2A-BlastR
Plasmid#172492PurposeFor CRISPR-assisted HDR, Hipp11 locusDepositorInsert, BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-ABE8e_dNRCH-BlastR
Plasmid#232720PurposeA-to-G base editorDepositorInsertABE8e-dCas9(NRCH)
UseTagsExpressionMammalianMutationwithin Cas9 D10A H840APromoterAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only
