171,532 results

Showing: 7041 - 7060 of 171532 results

Results per page:

Sort results by:

pCMV-eNme2-C-ABE8e

Plasmid

#185667

Purpose

Expresses eNme2-C-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-C

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag MKK4

Plasmid

#14615

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CamKII-TRPV1-P2A-mCherry

Plasmid

#200829

Purpose

Transduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporter

Depositor

Hong Chen

Article

Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

Insert

TRPV1-P2A-mCherry (Trpv1 Rat)

Use

AAV

Expression

Mammalian

Promoter

CamKII

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-sgRNA

Plasmid

#50662

Purpose

Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3b-8XGliBS:EGFP

Plasmid

#84602

Purpose

Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

Depositor

James Chen

Article

Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

Insert

eGFP

Expression

Mammalian

Promoter

8X Gli-binding site

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-cFos-tTA-pA

Plasmid

#66794

Purpose

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

cfos promoter linked to the tet activator gene

Use

AAV

Tags

no

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-NR2B

Plasmid

#17925

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

N-methyl D-aspartate receptor 2B (Grin2b Rat)

Tags

eGFP

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
GAPDH-CPG-TV

Plasmid

#70148

Purpose

a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh

Use

Mouse Targeting and Synthetic Biology

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)

Plasmid

#72638

Purpose

Tol2 based UAS modified with a CCR5TC binding site upstream of it, driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) repressor domain

Depositor

Joshua Bonkowsky

Insert

UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)

Use

Gateway

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE BRG1

Plasmid

#1959

Depositor

Robert Kingston

Article

Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

Insert

BRG1 (SMARCA4 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

E1262K R1575Q

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV- κLight1.3

Plasmid

#201223

Purpose

Express kLight1.3 biosensor in mammalian cells

Depositor

Lin Tian

Article

Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

Insert

κLight1.3

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-TYRO3-V5/HIS

Plasmid

#200118

Purpose

Expression of human TYRO3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human TYRO3 receptor tyrosine kinase (TYRO3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPEP-TEV cNup98 (1-498)

Plasmid

#38037

Depositor

Roderick Lim

Article

Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

Insert

Nucleoporin 98 (NUP98 Human)

Tags

His-tag, 3Cysteine

Expression

Bacterial

Mutation

amino acids 1-498

Promoter

T7

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP332 (Lenti-OSK1)

Plasmid

#21627

Depositor

Tim Townes

Article

Chang et al Stem Cells. 2009 May . 27(5):1042-9.

Insert

hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Porcine teschovirus-1, Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-YE1-BE4max

Plasmid

#198546

Purpose

Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET his-mCherry

Plasmid

#113552

Purpose

For bacterial expression and purification of mCherry

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

his-mCherry

Expression

Bacterial

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
H3K9me3 biosensor

Plasmid

#120802

Purpose

FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP

Plasmid

#21181

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

enhanced green flourescent protein

Use

Drosophila expression

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-Blue FP

Plasmid

#52115

Purpose

a bicistronic IRES-Blue FP containing retroviral vector

Depositor

Dario Vignali

Type

Empty backbone

Use

Retroviral

Tags

IRES-Blue FP

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge