We narrowed to 18,135 results for: URE

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pHH0103_SHANK3-1/1

Plasmid

#91421

Purpose

Protein expression and purification of human SH3 domain construct SHANK3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SHANK3-1/1 (SHANK3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSBK.013

Plasmid

#187213

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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B2387_mEGFP-FKBP2x

Plasmid

#216216

Purpose

Expresses mEGFP(A206K) fused to FKBP-FKBP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

mEGFP-FKBP-FKBP (FKBP1A Human)

Tags

EGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-Flag-Mili-EGFP

Plasmid

#212576

Purpose

Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

Mili (Piwil2 Mouse)

Use

Lentiviral

Tags

5x Flag and SNAP tag

Expression

Mammalian

Promoter

SFFV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

Plasmid

#149426

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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Arp2_pLib

Plasmid

#173676

Purpose

Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp2 (ACTR2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLXSN-FLK1 TM

Plasmid

#65249

Purpose

Expression of dominant negative FLK1 in mammalian cells

Depositor

Axel Ullrich

Article

Millauer et al Nature. 1994 Feb 10;367(6463):576-9.

Insert

FLK1 TM (Kdr Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

deleted AA 786-1346

Promoter

LTR

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pET28b(+)-SPN+IFS

Plasmid

#83372

Purpose

Expresses SPN and IFS complex in E. coli cell

Depositor

SeWon Suh

Article

Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

Inserts

SPN
IFS

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN-HA (murine)

Plasmid

#130915

Purpose

Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN murine

Tags

HA

Expression

Mammalian

Mutation

murine cGAS catalytic domain (aa148-507)

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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MK13_HUMAN_D0

Plasmid

#79693

Purpose

This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK13

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pSBK.014

Plasmid

#187215

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-Foxo1-WT 6KR

Plasmid

#17558

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KR (Foxo1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

K242R, K246R, K259R, K262R, K271R, K291R

Promoter

CMV

Available Since

Oct. 8, 2008

Availability

Academic Institutions and Nonprofits only
pHH0103_SH3GL3-1/1

Plasmid

#91423

Purpose

Protein expression and purification of human SH3 domain construct SH3GL3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GL3-1/1 (SH3GL3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEBG-MKK4 (GST)

Plasmid

#21563

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Tags

GST

Expression

Mammalian

Available Since

July 17, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_mGFP-FGFR3-mScarlet

Plasmid

#192509

Purpose

Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc-mScarlet

Tags

mGFP and mScarlet

Expression

Mammalian

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A66V

Plasmid

#166817

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

A66V

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 FOXM

Plasmid

#67118

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

FOXM (FOXM1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221 NLRP1 S1213A

Plasmid

#166824

Purpose

Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

S1213A

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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PAK7_HUMAN_D0

Plasmid

#79706

Purpose

This plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PAK7 (PAK5 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EGFR∆550-580-mEGFP

Plasmid

#203788

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

EGFR (EGFR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

aa 550-580 deleted

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_TNK1-1/1-SV1/3

Plasmid

#91548

Purpose

Protein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK1-1/1-SV1/3 (TNK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-AMV-α1L9'S-nAchR

Plasmid

#20684

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrna1 Mouse)

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Available Since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pJRTCLCrVA010

Plasmid

#22843

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA containing GFP

Use

Cotton expression vector

Expression

Bacterial

Mutation

Cotton Leaf Crumple Virus A component with Coat P…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-C-Kbp

Plasmid

#178013

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-C-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-C-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Pa-Kbp

Plasmid

#178014

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Pa-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Pa-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Hv-Kbp

Plasmid

#178015

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Hv-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Hv-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HisD-mNG-D-Kbp

Plasmid

#178012

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-D-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-D-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a-SRAP-delCys2

Plasmid

#84568

Purpose

Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Srap1 (Hmces Mouse)

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

deletion of Cys2

Promoter

T7

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-NMM-tdtomato

Plasmid

#129557

Purpose

pClimDC-NMM:tdT cell membrane marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

N-Myristoylation motif (NMM) fused to tdtomato

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic

Plasmid

#32736

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only