165,307 results

1. pLVX-RNaseHI-NES-EGFP

   Plasmid

   #196701

   Purpose

   Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   bacterial RNaseHI
   

   Use

   Lentiviral

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MtPT4-p3

   Plasmid

   #160003

   Purpose

   Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Inserts

   DsRed (reverse orientation)
   DODAα1
   CYP76AD1
   cDOPA5GT
   

   Expression

   Plant

   Promoter

   Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUCP20T-tdTomato

   Plasmid

   #78469

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   tdTomato
   

   Expression

   Bacterial

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAd5-B6/7 ∆E3-GFP

   Plasmid

   #179202

   Purpose

   A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.

   Depositor

   Fred Bunz

   Article

   Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

   Insert

   Modified block 6/7, with deletion in E3 and insertion of GFP cassette
   

   Use

   Adenoviral

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-Flag-Tet1 CD

   Plasmid

   #70129

   Purpose

   Express flag-tagged mouse Tet1 catalytic domain.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET1 catalytic domain.

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBbS6c-RFP

   Plasmid

   #35293

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   LlacO-1

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGG-BC-TurboID-GOI

   Plasmid

   #209390

   Purpose

   Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGG-GOI-DE-TurboID

   Plasmid

   #209388

   Purpose

   Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. U6-sgfiller-eCas9-T2A-BlastR

   Plasmid

   #172437

   Purpose

   Expresses eCas9, BlastR and sgRNA cloning site

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Inserts

   eCas9
   BlastR
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SF-SON IRES Tomato

    Plasmid

    #214403

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-MRLC1

    Plasmid

    #35680

    Depositor

    Tom Egelhoff

    Article

    Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

    Insert

    MRLC1 (MYL9 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pGWB551

    Plasmid

    #74881

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

    Plasmid

    #115684

    Purpose

    NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

    Depositor

    David Suter

    Article

    Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

    Insert

    Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
    

    Use

    Lentiviral

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTHSSe_44

    Plasmid

    #109242

    Purpose

    pSC101 var2 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBbi 41BBL-CD86

    Plasmid

    #183244

    Purpose

    Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    CD86, 41BBL
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. qTAG-N-Solo-mScarlet-LMNB1

    Plasmid

    #207771

    Purpose

    Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. BmTyr-Flag

    Plasmid

    #220779

    Purpose

    non-targeted BmTyr throughout the cytoplasm

    Depositor

    Itaru Hamachi

    Article

    Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

    Insert

    Bacillus megaterium Tyrosinase
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ES-FUCCI

    Plasmid

    #62451

    Purpose

    SMX_DEST vector with 3 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
    minPGK::Cherry-hCdt1(30-120)-bGHpA
    PGK::HygroR-bGpA
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-Cas9-T2A-TdT

    Plasmid

    #126424

    Purpose

    Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-TdT (DNTT Streptococcus pyogenes, Human)
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHCMV_SFV_E1E2

    Plasmid

    #207308

    Purpose

    Mammalian expression of Semliki Forest virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    SFV_E1E2
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Tol2_8xCTS-ECFP

    Plasmid

    #200249

    Purpose

    Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    8xCTS-ECFP
    

    Use

    Zebrafish expression

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AMPK(T>A)-SPARK

    Plasmid

    #226256

    Purpose

    phospho-null mutant of AMPK-SPARK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK(T>A)-SPARK
    

    Expression

    Mammalian

    Mutation

    substituted Threonine 20 with Alanine

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTALYM4SpMi-05

    Plasmid

    #47880

    Purpose

    TALE-mRuby2 against Mus spretus/musculus minor satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. bactin2:loxP-STOP-loxP-DsRed-express

    Plasmid

    #68797

    Purpose

    For making transgenic fish

    Depositor

    David Traver

    Article

    Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

    Inserts

    beta-actin promoter
    loxp-stop-codon-loxp-dsred
    

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDM304_DPAKa(GBD)-YFP

    Plasmid

    #128699

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Mutation

    none

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAK_DR30_CasRx_Puro

    Plasmid

    #134845

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A Puromycin

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEN-sacB

    Plasmid

    #196249

    Purpose

    Plasmid containing the sacB gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    attR2-sacB-attL3
    

    Expression

    Bacterial

    Promoter

    sacB promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA-FLAG-METTL3-APPA

    Plasmid

    #160251

    Purpose

    Express catalytic inactive FLAG-METTL3 in human cells

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changes in aa 395–398, DPPW → APPA

    Promoter

    CMV

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3XFLAG-TAK1-K63A

    Plasmid

    #43933

    Depositor

    Bryant Darnay

    Article

    Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

    Insert

    Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    K63A

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge