We narrowed to 59,208 results for: Ran

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pAC91-pmax-dCas9VP64

Plasmid

#48223

Purpose

dCas9VP64 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP64 activation domain

Use

CRISPR

Tags

HA Tag and VP64

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

Plasmid

#193854

Purpose

Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

5' Cbr-unc-119(+) + 5' mNeonGreen

Use

CRISPR and Cre/Lox

Expression

Worm

Promoter

Cbr-unc-119p

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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LC101: pMVP/PB/Neo-DEST

Plasmid

#121875

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

Plasmid

#174884

Purpose

Targeting vector for generating Sox2 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Sox2

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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CIBN-GFP-TMD

Plasmid

#79571

Purpose

expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

syntaxin 1A (Stx1a Mouse)

Tags

CIBN and EGFP

Expression

Mammalian

Mutation

aa 249_288 (C-terminal region (polybasic stretch …

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TIE-V5/HIS

Plasmid

#205612

Purpose

Expression of human TIE receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TIE receptor tyrosine kinase (TIE1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pES4/4a

Plasmid

#69590

Purpose

TCR a chain cDNA in pES4 transgenic construct for creating OT-II mice

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Insert

TCR alpha (Tcra Mouse)

Expression

Mammalian

Promoter

H-2Kb

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only
pU6-sgGAL4-1

Plasmid

#46915

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-1
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

Plasmid

#158987

Purpose

Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP160

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP2

Plasmid

#17793

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 MYC dHLH

Plasmid

#176047

Purpose

Expresses Myc Mutant (delta helix-hoop-helix) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Expression

Mammalian

Mutation

deleted amino acids 371-412

Promoter

CMV

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11B-HA

Plasmid

#209219

Purpose

Mammalian expression of ATP11B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP11B (ATP11B Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW1

Plasmid

#203163

Purpose

Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAWH-largeT

Plasmid

#133895

Purpose

Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

LargeT

Expression

Yeast

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Cerulean-GalT

Plasmid

#11930

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

Plasmid

#113890

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pQlinkH-PTPIP51(36-235)

Plasmid

#170533

Purpose

Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

His tag

Expression

Bacterial

Promoter

T5 promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper-T7IS1

Plasmid

#140631

Purpose

Expresses ShCAST under the T7 promoter and an empty sgRNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pOP/SSAT

Plasmid

#140291

Purpose

Expresses human SSAT in bacterial cells

Depositor

Saverio Bettuzzi, Gaetano Marverti

Article

Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSL0812 (pCRISPR(BbsI)_Vch)

Plasmid

#200898

Purpose

Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCRISPR (entry)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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