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pCW57.1-mDux-CA

Plasmid

#99284

Purpose

All-in-one lentivector with tet-inducible mouse Dux (codon sequences altered)

Depositor

Stephen Tapscott

Article

Whiddon et al Nat Genet. 2017 Jun;49(6):935-940. doi:

Insert

Dux-CA (mouse; codon altered) (Dux Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-CMVSP6

Plasmid

#138356

Purpose

Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

CMV-SP6 promoter

Use

Gateway compatible

Promoter

CMV-SP6

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTK_Msx1_enh_264_Citrine

Plasmid

#130610

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Msx1_enh_264

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHA-4EHP

Plasmid

#17344

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
pTK_TFAP2a_enh_249_Cerulean

Plasmid

#130615

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

TFAP2a_enh_249

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pL6puro-pmRevErbA-Venus-NLS-Pest

Plasmid

#240105

Purpose

A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein)

Depositor

Achim Kramer

Article

Gabriel et al bioRxiv 2025.06.30.661926

Insert

murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)

Use

Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTK_Axud1_enh_394_Citrine

Plasmid

#130625

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Axud1_enh_394

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-tdMCP-msfGFP(V206K)-GBI

Plasmid

#205163

Purpose

express tdMCP-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

tdMCP-msfGFP(V206K)-GBI

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP

Plasmid

#69536

Purpose

Episomal plasmid encoding dCas9VP192

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-EGFP

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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NRIP1_pCSdest

Plasmid

#53805

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

NRIP1 (NRIP1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pQE30-mBFP

Plasmid

#112682

Purpose

Expression of NADPH-dependent blue fluorescent protein

Depositor

Geun-Joong Kim

Article

Hwang et al Biochem Biophys Res Commun. 2012 Mar 23;

Insert

His-mBFP

Tags

His tag

Expression

Bacterial

Promoter

T5

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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CDS12_mTurquoise2

Plasmid

#136089

Purpose

FP for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mTurquoise2(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMM146

Plasmid

#127218

Purpose

BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, BBM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSP6-EBNA(2A+DBD)

Plasmid

#98749

Purpose

In vitro transcription of modified EBNA1 mRNA

Depositor

Sara Howden, Jim Vadolas

Article

Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

Insert

Modified EBNA1

Use

In vitro transcription

Promoter

SP6

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-memRFP

Plasmid

#224564

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

mRFP1

Use

Unspecified

Tags

Membrane localization signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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RP4-11

Plasmid

#79812

Purpose

Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid

Depositor

Michael Hynes

Article

Quandt et al Plasmid. 2004 Jul;52(1):1-12.

Insert

Kanamycin cassette and SacB gene (sacB )

Use

Helper plasmid for mobilization

Promoter

Km

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only
pDHt-SK

Plasmid

#63615

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g1

Plasmid

#80784

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-i53-bpA-EF1BFP

Plasmid

#111145

Purpose

Expression of i53 and a BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

i53 (mutL Synthetic)
BFP (BFP Synthetic)

Expression

Mammalian

Mutation

human Ubiquitin, contains 8 point mutations

Promoter

CAG promoter and EF1

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2118

Plasmid

#49143

Purpose

AAV plasmid with smCBA promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Mirjalili Mohanna et al Gene Ther. 2023 Apr 19. doi: 10.1038/s41

Insert

ssAAV-smCBA-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

smCBA

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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