We narrowed to 30,735 results for: PLE

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pAGM55273

Plasmid

#153211

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag hDPY30

Plasmid

#15554

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

DPY30 (DPY30 Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsDCP2

Plasmid

#25031

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP2 (DCP2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP670nano3

Plasmid

#184670

Purpose

Histone 2B labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

H2B-miRFP670nano3

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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IgK-IFN-luc

Plasmid

#14886

Purpose

Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

Depositor

David Baltimore

Article

Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

Insert

IgK-IFN (IFNB1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin HL

Plasmid

#68128

Purpose

nesprin headless control

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

nesprin-2 (Syne2 Mouse)

Expression

Mammalian

Mutation

deleted actin-binding domain

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSMART-Ala1

Plasmid

#65814

Purpose

NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Insulated waaHp from E. coli

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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myrAkt delta4-129

Plasmid

#10841

Depositor

Richard Roth

Article

Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

Insert

Akt (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Mutation

Deletion of PH domain (aa4-129)

Promoter

SV40

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pLYS5-MCU-Flag

Plasmid

#50054

Purpose

Expression of human MCU in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MCU-Flag (MCU Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- Po Long P2A

Plasmid

#164117

Purpose

Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP Mt-ER- Po Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Long P2A

Plasmid

#164115

Purpose

Detect the long Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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CD8a

Plasmid

#86050

Purpose

A mammalian expression plasmid encoding CD8a without tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Expression

Mammalian

Promoter

CMV, T7

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGEL1029

Plasmid

#225600

Purpose

The STU backbone vector for tomato genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMXSIP-CMTM6-HA

Plasmid

#204710

Purpose

expresses CMTM6-HA in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

CMTM6 (CMTM6 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL

Plasmid

#182012

Purpose

CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

CalR-HaloTag7-P30-SNAP-tag-KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shRNA E-cadherin

Plasmid

#18801

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin shRNA (CDH1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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AIO-GFP

Plasmid

#74119

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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VP64-dCas9

Plasmid

#177171

Purpose

VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

VP64-dCas9(D10A, H840A)

Use

CRISPR

Tags

NLS and NLS-3xFLag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MT363

Plasmid

#65858

Purpose

The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

nahG

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Paxillin-22

Plasmid

#57153

Purpose

Localization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Paxillin

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEGFP-N1-ACTR3

Plasmid

#8462

Depositor

Matthew Welch

Article

Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

Insert

ACTR3 (ACTR3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Tags

Polyhistidine-TEV

Expression

Insect

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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act5C-mScarlet

Plasmid

#230906

Purpose

Ubiquitous expression of mScarlet in Drosophila melanogaster

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

mScarlet

Expression

Insect

Promoter

act5C

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12

Plasmid

#67514

Purpose

FKBP12 only

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12 (FKBP1A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-(SUMO)3

Plasmid

#127093

Purpose

Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Tags

EGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Promoter

DHFR-TS 5' UTR

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAIP Nup62Fv2GFP

Plasmid

#139684

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an SFFV promoter

Depositor

Edward Campbell

Article

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET11a-hRanGAP1

Plasmid

#50941

Purpose

expression of full length untagged human RanGAP1 in E.coli

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanGTPase activating protein 1 (RANGAP1 Human)

Expression

Bacterial

Promoter

T7

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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CapChR2_pmScarlet-N1

Plasmid

#188032

Purpose

Expresses CapChR2-mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Tags

mScarlet

Expression

Mammalian

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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CD8a-EGFP

Plasmid

#86051

Purpose

A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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