We narrowed to 3,042 results for: NAK

1. pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

   Plasmid

   #123658

   Purpose

   DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-CAGGS-mNAGA

   Plasmid

   #196448

   Purpose

   Expresses modified α-N-acetylgalactosaminidase in mammalian cells

   Depositor

   Yuichiro Miyaoka

   Article

   Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897

   Insert

   modified alpha-N-acetylgalactosaminidase (NAGA Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRvCAHS1-mEGFP-NLS

   Plasmid

   #205013

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRvSAHS1-mCherry-NLS

   Plasmid

   #205009

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1120 pBabe puroL HA FKHR H215R

   Plasmid

   #9026

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR H215R (FOXO1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H215R

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pONSY-mCherry

   Plasmid

   #111874

   Purpose

   This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDsRed-HP1alpha

   Plasmid

   #179702

   Purpose

   visualization of centromere heterochromatin

   Depositor

   Kenji Sugimoto

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2014;78(4):55

   Insert

   heterochromatin protein alpha (CBX5 Human)
   

   Tags

   dsRed

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSECB sgMTHFD2L_1

   Plasmid

   #106306

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2L (MTHFD2L Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSECB sgMTHFD2L_2

   Plasmid

   #106307

   Purpose

   Express Cas9 and sgRNA targeting MTHFD2L

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting MTHFD2L (MTHFD2L Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pONSY-Lifeact:mCherry

    Plasmid

    #111876

    Purpose

    This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Lifeact fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pONSY-Venus

    Plasmid

    #111875

    Purpose

    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. dmBACCS2NS-IRES-dOrai

    Plasmid

    #67628

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMT-Tyrosinase-bait-T2a-Cherry-pA

    Plasmid

    #111155

    Purpose

    knock in bait for Axolotl Tyrosinase locus

    Depositor

    Elly Tanaka

    Article

    Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

    Insert

    Tyrosinase-bait-T2a-Cherry-pA
    

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBCMV-MS2CP-eDHFR

    Plasmid

    #167309

    Purpose

    Template pDNA to prepare a split CaVT component mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    MS2CP-eDHFR
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)_UG3

    Plasmid

    #133874

    Purpose

    Expresses UG3 in mammalian cells.

    Depositor

    Sumio Terada

    Article

    Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

    Insert

    UtrCH (UTRN Human)
    

    Tags

    helical linker - mEGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. ECFP-FRB-Sec61b

    Plasmid

    #210290

    Purpose

    nucleus OM marker and "anchor" for FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    ECFP-FRB-Sec61b
    

    Tags

    ECFP and FRB

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tom20-EYFP-miLID

    Plasmid

    #210291

    Purpose

    mitochondria marker and "anchor" for optogenetics

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    Tom20-EYFP-miLID
    

    Tags

    EYFP and iLID

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mActA-mSspB-mCherry

    Plasmid

    #210294

    Purpose

    ActuAtor for iLID optogenetics

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    mActA-,SspB-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-myc-GFP-TNRC6A-SD-WT

    Plasmid

    #215897

    Purpose

    Expression of TNRC6A-silencing domain (SD)

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

    Plasmid

    #215898

    Purpose

    Expression of TNRC6A-CIM1 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

    Plasmid

    #215899

    Purpose

    Expression of TNRC6A-CIM2 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1529-1709

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A

    Plasmid

    #215900

    Purpose

    Expression of TNRC6A-CIM1 domain with S1332A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528, S1332A

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A

    Plasmid

    #215901

    Purpose

    Expression of TNRC6A-CIM1 domain with S1346A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528, S1346A

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-C1-IFT57

    Plasmid

    #218728

    Purpose

    Expresses N-terminally EGFP-tagged IFT57 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Funabashi et al J Cell Biol. 2018 Aug 6;217(8):2867-2876

    Insert

    IFT57 (IFT57 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFP-N2-IFT20

    Plasmid

    #218719

    Purpose

    Expresses C-terminally EGFP-tagged IFT20 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT20 (IFT20 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEGFP-C1-BBS9

    Plasmid

    #218717

    Purpose

    Expresses N-terminally EGFP-tagged BBS9 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS9 (BBS9 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A455T (g1876a)

    Promoter

    CMV

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEGFP-C1-IFT38

    Plasmid

    #218723

    Purpose

    Expresses N-terminally EGFP-tagged IFT38 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT38 (CLUAP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEGFP-N3-hARL1(Q71L)

    Plasmid

    #206407

    Purpose

    Mammalian expression of hARL1(Q71L)

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

    Insert

    ARL1 (ARL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q71L

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP-N3-hARL1(T31N)

    Plasmid

    #206408

    Purpose

    Mammalian expression of hARL1(T31N)

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

    Insert

    ARL1 (ARL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T31N

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCE-hOCT3/4

    Plasmid

    #41813

    Purpose

    Non-integrating (episomal) expression of human OCT3/4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    POU5F1 (POU5F1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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