Addgene

We narrowed to 9,510 results for: Gnas

Showing: 1751 - 1800 of 9510 results

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CS2 Flag-Smad3 (3A)

Plasmid

#14964

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (3A) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

Three Ser to Ala mutations in the phosphorylation…

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pSK-c-mpl

Plasmid

#1828

Depositor

Philip Leder

Article

Skoda et al EMBO J 1993 Jul;12(7):2645-53.

Insert

c-mpl (Mpl Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
YF-Rac1(DN)

Plasmid

#20151

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Rac1(DN) (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

dominant negative mutation

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs12

Plasmid

#109357

Purpose

GsX chimera with Gα12 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs12 chimera

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs15

Plasmid

#109360

Purpose

GsX chimera with Gα15 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs15 chimera

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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SEC13 in pRK5

Plasmid

#154215

Purpose

Untagged SEC13 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

Sec13

Use

Tags

NONE

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKCzeta.RNAi

Plasmid

#10803

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC zeta RNAi (PRKCZ Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
HT-mGold

Plasmid

#183986

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells in the whole cell.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

HaloTag-mGold

Use

Tags

HaloTag, mGold

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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PD23

Plasmid

#164536

Purpose

Expresses SmoM2-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened W539L (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

W539L

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pWS484

Plasmid

#123032

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (2x) [2a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS483

Plasmid

#123031

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (3x) [2a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS482

Plasmid

#123030

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (4x) [2a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12B

Plasmid

#87079

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12B (Trim5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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zWnt5b

Plasmid

#21282

Depositor

Randall Moon

Article

Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

Insert

Wnt5b (wnt5b Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only

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pHR Src-TagBFP

Plasmid

#171036

Purpose

TagBFP-tagged Src kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM30A

Plasmid

#87081

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30A (Trim30a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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36 p flk1

Plasmid

#13061

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

flk1 (kdrl Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
LPCX Smad3 deltaSSVS

Plasmid

#12639

Depositor

Rik Derynck

Article

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Last 4 aa's deleted.

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pGKO2

Plasmid

#63617

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Tags

Expression

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5B ActRIB-HA T206D

Plasmid

#27223

Depositor

Joan Massague

Article

Attisano et al Mol Cell Biol. 1996 Mar . 16(3):1066-73.

Insert

ActRIB (ACVR1B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

T206D

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pAGM9121_pCAT1

Plasmid

#160918

Purpose

Catalase 1 (CAT1) promoter module for episomal P. pastoris promoter shuffling

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

Catalase 1 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER.PKD2.RNAi

Plasmid

#10816

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKD2 RNAi (PRKD2 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
MyrPalm-BKAR

Plasmid

#14878

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Use

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
BGGLO QPCR PLASMID

Plasmid

#79905

Purpose

Encodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..

Depositor

Jeffery Miller

Article

Bhanu et al Blood. 2004 Mar 1;103(5):1929-33. Epub 2

Insert

Human partial HBG and HBB gene

Use

Tags

Expression

Mutation

Promoter

No promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS1837

Plasmid

#123058

Purpose

_-factor pheromone

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

_-factor [3-4a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEBB HA BIR 1-2-3

Plasmid

#25692

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

aa 1-350

Promoter

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pMpGWB102-Citrine-PTS1

Plasmid

#44592

Purpose

Binary vectors for expression of Citrine fluorescent protein with a peroxisome localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Use

Tags

PTS1 (peroxisomal targeting signal type 1)

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
dishevelled

Plasmid

#38342

Depositor

Norbert Perrimon

Article

Klingensmith et al Genes Dev. 1994 Jan;8(1):118-30.

Insert

dishevelled (dsh Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pEBB-HA-hILP

Plasmid

#25674

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pCph8

Plasmid

#50552

Purpose

Expresses Cph8 under a modified PLtetO-1 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Insert

Cph8

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX mSmo-Cterm

Plasmid

#58702

Purpose

used for anti-mouse Smoothened antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

tac

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
pWS1037

Plasmid

#123049

Purpose

G_ subunit

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 [3-4a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-tVYN-Nt∆

Plasmid

#154431

Purpose

Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-NtYcgRΔ-NLucΔ4

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB HA BIR 2

Plasmid

#25688

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

aa 124-261

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
CMVTO-SEAP-26SFur-B2AD-tvmvs-KKMP

Plasmid

#179645

Purpose

TVMVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-GFP-26Sfur-3TM-hcvs-KKMP

Plasmid

#179642

Purpose

HCVP-inducible tri-transmembrane GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

GFP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mSprouty4 EST

Plasmid

#22093

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 4 (Spry4 Mouse)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pWS627

Plasmid

#123064

Purpose

G___ bicistronic protein

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste4-2A-Ste18 [3-4a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV VEET-GFP

Plasmid

#188706

Purpose

Lentivirus transfer plasmid encoding VEET-GFP

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

VEET-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p8267 LentiCRISPR v2 hygro sgNT-2

Plasmid

#193978

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pm-EphA1-RTK-Cry2olig

Plasmid

#176683

Purpose

membrane localized EphrinA1 RTK fusion to Cry2olig - RTK domain only

Depositor

Robert Hughes

Article

Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

Insert

PM-EphA1-Cry2olig-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRC063

Plasmid

#123057

Purpose

Blue fluorescent protein

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

mTagBFP2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP64TS porcine TGFbeta1 (DM#167)

Plasmid

#15011

Depositor

Douglas Melton

Article

Reilly et al Cell. 1996 Sep 6. 86(5):743-54.

Insert

TGFb type 1 (TGFB1 porcine (Sus scrofa))

Use

Sp6 expression for use in xenopus

Tags

Flag / HMK

Expression

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pQCXIP-Rx3(A6)-mEGFP

Plasmid

#35530

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Rx3(A6)mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB102-Citrine-NLS

Plasmid

#79136

Purpose

Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-NLS

Use

Tags

NLS (Nuclear Localization Signal)

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5B ActR-IB

Plasmid

#14050

Depositor

Joan Massague

Article

Attisano et al Mol Cell Biol. 1996 Mar . 16(3):1066-73.

Insert

ActR-IB (ACVR1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pAG414GPD-VuCYP722C(pYL1070)

Plasmid

#178293

Purpose

Expresses VuCYP722C from V. unguiculata in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VuCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

VuCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP-Rx3(A8)-mEGFP

Plasmid

#35532

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Rx3A8mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB HA BIR 1

Plasmid

#25687

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

aa 1-124 (BIR 1)

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pRC246

Plasmid

#123038

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pLEU2m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1751 - 1800 of 9510 results

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