We narrowed to 6,296 results for: pcDNA 3.1
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Plasmid#232339PurposeExpresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340DepositorInsertIARS2 (IARS2 Human)
ExpressionMammalianMutationPutative tRNA binding deficiency mutation K664A A…PromoterCMVAvailable SinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S-ectodomain
Plasmid#235984PurposeInternally tagged SARS-CoV-2 spike ecto-domainDepositorInsertSpike protein (S SARS-CoV-2)
Tags8xHis and Twin-Strep tag and internal Twin-Strep …ExpressionMammalianMutationaa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…PromoterCMVAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-V561D-V5/HIS
Plasmid#234762PurposeExpression of the V561D mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor and GIST-Plus Syndrome, , constantly activatedDepositorInsertPDGFRA-V561D receptor tyrosine kinase, full length (PDGFRA Human)
TagsV5/HisExpressionMammalianMutationV561D substitutionPromoterCMVAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-P577S-V5/HIS
Plasmid#234760PurposeExpression of the P577S mutant variant of human PDGFRA, which might be associated with MelanomaDepositorInsertPDGFRA-P577S receptor tyrosine kinase, full length (PDGFRA Human)
TagsV5/HisExpressionMammalianMutationP577S substitutionPromoterCMVAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-R487L-V5/HIS
Plasmid#234761PurposeExpression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal TumorDepositorInsertPDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
TagsV5/HisExpressionMammalianMutationR487L substitutionPromoterCMVAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-D842V-V5/HIS
Plasmid#234763PurposeExpression of the D842V mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor, constantly activatedDepositorInsertPDGFRA-D842V receptor tyrosine kinase, full length (PDGFRA Human)
TagsV5/HisExpressionMammalianMutationD842V substitutionPromoterCMVAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-G853D-V5/HIS
Plasmid#234757PurposeExpression of the G853D mutant variant of human PDGFRA, which might be associated with MelanomaDepositorInsertPDGFRA-G853D receptor tyrosine kinase, full length (PDGFRA Human)
TagsV5/HisExpressionMammalianMutationG853D substitutionPromoterCMVAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-T674I-V5/HIS
Plasmid#234756PurposeExpression of the T674I mutant variant of human PDGFRA, which is associated with Idiopathic Hypereosinophilic Syndrome, resistant to imatinibDepositorInsertPDGFRA-T674I receptor tyrosine kinase, full length (PDGFRA Human)
TagsV5/HisExpressionMammalianMutationT674I substitutionPromoterCMVAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
YFP Rab11 Q70L in pcDNA3.1
Plasmid#90467PurposeRhoA mediated cell migrationDepositorInsertRab11 Q70L
TagsmEYFPExpressionMammalianPromoterCMVAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-K627M-V5/HIS
Plasmid#234755PurposeExpression of the K627M kinase defective mutant variant of human PDGFRADepositorInsertPDGFRA-K627M receptor tyrosine kinase, full length (PDGFRA Human)
TagsV5/HisExpressionMammalianMutationK627M substitutionPromoterCMVAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3
Plasmid#233062PurposeExpresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.DepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt
Plasmid#233240PurposeParallel coiled-coil with (GGS)3 linker to prone condensationDepositorInsertVh-clover-GCN4-(GGS)3-Vt
ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt
Plasmid#233241PurposeParallel coiled-coil with (GGS)12 linker to prevent condensationDepositorInsertVh-clover-GCN4-(GGS)12-Vt
ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FFSS-BACH1 C122E
Plasmid#232274PurposeExpression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell linesDepositorInsertBACH1 (BACH1 Human)
Tags2xFLAG-2xSTREPExpressionMammalianMutationchanged cysteine 122 to glutamic acidPromoterCMVAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-AP4-cyto
Plasmid#229694PurposeTransient expression of EGFP AP4-cyto in mammalian cellsDepositorInsertEGFP; Control construct with tandem AP4 motifs (cytoplasm)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-FP4-cyto
Plasmid#229695PurposeTransient expression of FP4-cyto in mammalian cellsDepositorInsertEGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc:FRB:gs10:Cul1(dC501)
Plasmid#228533PurposeA fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc:Rbx1:gs10:FRB:HA
Plasmid#228534PurposeHuman Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorInsertmyc:Rbx1:gs10:FRB:HA (RBX1 Synthetic, Human)
TagsHA and mycExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-NbON10-NanoLuc
Plasmid#198691PurposeExpresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell cultureDepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
Tags6xHis tag, IgK leader sequence, NanoLuc, and twin…ExpressionMammalianPromoterCMV promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-8D2Q.Tau2N4R-WPRE
Plasmid#226382PurposeExpresses tau propagation ORF with V5 tag on 8D2Q mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutation8D2Q.Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-20D3Q.Tau2N4R-WPRE
Plasmid#226383PurposeExpresses tau propagation ORF with V5 tag on 20D3Q mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutation20D3Q, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-8A2R.Tau2N4R-WPRE
Plasmid#226384PurposeExpresses tau propagation ORF with V5 tag on 8A2R mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutation8A2R, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-20A3R.Tau2N4R-WPRE
Plasmid#226385PurposeExpresses tau propagation ORF with V5 tag on 20A3R mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutation20A3R, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-K281Q.Tau2N4R-WPRE
Plasmid#226386PurposeExpresses tau propagation ORF with V5 tag on K281Q mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutationK281Q, 2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-K353Q.Tau2N4R-WPRE
Plasmid#226387PurposeExpresses tau propagation ORF with V5 tag on K353Q mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutationK353Q, 2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-S198D.Tau2N4R-WPRE
Plasmid#226388PurposeExpresses tau propagation ORF with V5 tag on S189D mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutationS198D.Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-S199D.Tau2N4R-WPRE
Plasmid#226389PurposeExpresses tau propagation ORF with V5 tag on S199D mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T-2A-V5ExpressionMammalianMutationS199D, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-S202D.Tau2N4R-WPRE
Plasmid#226390PurposeExpresses tau propagation ORF with V5 tag on S202D mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutationS202D, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-S404D.Tau2N4R-WPRE
Plasmid#226391PurposeExpresses tau propagation ORF with V5 tag on S404D mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutationS404D, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-T217D.Tau2N4R-WPRE
Plasmid#226392PurposeExpresses tau propagation ORF with V5 tag on T217D mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutationT217D, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-T403D.Tau2N4R-WPRE
Plasmid#226393PurposeExpresses tau propagation ORF with V5 tag on T403D mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutationT403D, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-T181D.Tau2N4R-WPRE
Plasmid#226394PurposeExpresses tau propagation ORF with V5 tag on T181D mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutationT181D, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-).TagRFP.T-2A-V5-T231D.Tau2N4R-WPRE
Plasmid#226395PurposeExpresses tau propagation ORF with V5 tag on T231D mutant 2N4R tauDepositorInsertTau2N4R (MAPT Human, synthetic)
TagsTagRFP.T.2A.V5ExpressionMammalianMutationT231D, Tau2N4RAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AntiHer2-HC121-TAG
Plasmid#226836PurposeContains trastuzumab with a TAG codon at position 121 of the heavy chain for ncAA incorporationDepositorInsertsanti-HER2 light chain
anti-HER2 heavy chain
UseAffinity Reagent/ AntibodyExpressionMammalianMutationamino acid 121 of heavy chain mutated to TAGAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)
Plasmid#187727PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
TagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 226-382Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)
Plasmid#187728PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
TagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 418-495Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-WT
Plasmid#215897PurposeExpression of TNRC6A-silencing domain (SD)DepositorAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-CAMK2D
Plasmid#221686PurposeMammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioIDDepositorInsertCAMK2D (CAMK2D Human)
ExpressionMammalianAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-RalA(G23C)
Plasmid#224275Purposecell transfection and expreswsion of RalA (G23C)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Light chain
Plasmid#216297PurposeExpresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S FabDepositorInsert39S Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Light chain
Plasmid#216304PurposeExpresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GST-EGFP-GPIDAF
Plasmid#213722PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
TagsV5-tag-GSTExpressionMammalianPromoterCMVAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GST-EGFP-GPICEAM7
Plasmid#213723PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter.DepositorInsertEGFP
TagsV5-tag-GSTExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA
Plasmid#214614Purposeadapter for RTK split TEV recruitment assaysDepositorAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIDAF
Plasmid#213712PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.DepositorInsertEGFP
TagsTwin-strep-tag and V5-tagExpressionMammalianPromoterSV40Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TST-EGFP-GPIBY55
Plasmid#213711PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.DepositorInsertEGFP
TagsTwin-strep-tag and V5-tagExpressionMammalianPromoterSV40Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1015A-E1021A
Plasmid#213417PurposeVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
ExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1015A-E1021A
Plasmid#213414PurposeVinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnCS-E1015A-E1021A (VCL Synthetic, Chicken)
ExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-62
Plasmid#208647PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
UseCRISPRExpressionMammalianPromoterCMV and U6 in tandemAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus R200W
Plasmid#209179PurposeMammalian expression of SPAX8-related missense mutation (R200W) in human NKX6-2 fused with the Venus fluorescent proteinDepositorInsertNKX6-2 (R200W) fused with Venus (NKX6-2 Human)
TagsVenusExpressionMammalianMutationNKX6-2 (R200W)PromoterCMVAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only