165,313 results

1. lenti EF1a-FLAG-dCas9-VPR

   Plasmid

   #114195

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX4T-hLIG3

   Plasmid

   #81055

   Purpose

   bacterial expression of human LIG3

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   DNA ligase 3 (LIG3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber, David Schaffer

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGP-AAV-syn-iGABASnFR2n-WPRE

   Plasmid

   #218875

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   Synapsin

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRE118

   Plasmid

   #43830

   Depositor

   Dieter Schifferli

   Article

   Edwards et al Gene. 1998 Jan 30;207(2):149-57.

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb1

   Expression

   Bacterial

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PiggyBac-TetO-En1-t2a-Foxa2-hygro

   Plasmid

   #176483

   Purpose

   TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   En1-t2a-FoxA2 (FOXA2, En1 Human, Mouse)
   

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PINK1-SmBiT Vector

   Plasmid

   #238782

   Purpose

   Express PINK1-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   PINK1 (PINK1 Human)
   

   Tags

   SmBiT

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Sept. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. p2T-TetON-Hygro-Arhgef11-Cry2-mCh

   Plasmid

   #240798

   Purpose

   Dox-inducible expression of Arhgef11-Cry2-mCherry

   Depositor

   Hans Clevers

   Article

   Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

   Insert

   Arhgef11-Cry2-mCherry
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   none

   Promoter

   TRE

   Available Since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFUW-TetO-NOTCH2

   Plasmid

   #207193

   Purpose

   doxycycline-inducible expression of Human NOTCH2 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

   Insert

   pFUW-TetO-NOTCH2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ACBE-NW

    Plasmid

    #240624

    Purpose

    A-to-C base editor with narrowed editing window

    Depositor

    Tingting Jiang

    Article

    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

    Insert

    TadA8e(N108Q, M151C, Q154Y)-nCas9 (D10A)-mAAG
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    TadA8e(N108Q, M151C, Q154Y),Cas9 (D10A),mAAG (R16…

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD24-ALFA-TwinStrep

    Plasmid

    #240248

    Purpose

    Arabinose-inducible plasmid with ALFA and Twin-Strep tags

    Depositor

    Olga Boudker, Krishna Reddy

    Article

    Reddy et al Nat Struct Mol Biol (2025).

    Type

    Empty backbone

    Tags

    10x HIS, ALFA, Thrombin, and Twin-Strep

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentiCCAT1-Red

    Plasmid

    #226521

    Purpose

    CCAT1 transgene with fluorescent protein mKate2

    Depositor

    Neville Sanjana

    Article

    Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

    Insert

    EF1a_mKate2_CMV_CCAT1 (CCAT1 Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV6-HCR-hAAT-SEAP-opt

    Plasmid

    #237873

    Purpose

    AAV vector / packaging plasmid: This product-specific plasmid provides a reporter encoding vector genome cassette and the AAV6 capsid gene.

    Depositor

    Ascend Advanced Therapies, Florian Sonntag

    Insert

    SEAP encoding AAV2 vector and cap6
    

    Use

    AAV

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-3_Flag-YTHDF2

    Plasmid

    #238975

    Purpose

    Expresses YTHDF2

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMVRha

    Plasmid

    #229553

    Purpose

    Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

    Depositor

    Sandy Schmidt

    Article

    Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 8138-E02

    Plasmid

    #225659

    Purpose

    Gateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    SORE6
    

    Use

    Gateway entry clone

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

    Plasmid

    #229545

    Purpose

    Lentiviral construct to express rtTA and SKM proteins

    Depositor

    Domitilla Del Vecchio

    Article

    Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

    Insert

    rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5 BMPS WT-TEV-6xHis

    Plasmid

    #204837

    Purpose

    Mammalian expression of recombinant BMPS WT

    Depositor

    Monther Abu-Remaileh

    Article

    Medoh et al Science. 2023 Sep 15;381(6663):1182-1189

    Insert

    CLN5 (CLN5 Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB_EF1a_Gag-EIF4E-TM

    Plasmid

    #212658

    Purpose

    piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Piggybac

    Tags

    EIF4E (W56L, W102L, W166L)

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. WT_S1-H1

    Plasmid

    #218392

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MUT_S1-H1

    Plasmid

    #218394

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYSD063

    Plasmid

    #212916

    Purpose

    Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSCR-YFP-GW

    Plasmid

    #209366

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis SCR promoter driving expression of N-terminal YFP fusion protein.

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pWOL-YFP-GW

    Plasmid

    #209365

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis WOL promoter driving expression of N-terminal YFP fusion protein.

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-eIF4E-TwStrep

    Plasmid

    #208638

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

    Depositor

    Quanfu Ma

    Insert

    human eIF4E (ENST00000450253) (EIF4E Human)
    

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.4_1G11F2-3 heavy chain

    Plasmid

    #198253

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)
    

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1B-lambdaN-HA-HseIF4AI_T

    Plasmid

    #147543

    Purpose

    Insect Expression of HseIF4AI

    Depositor

    Elisa Izaurralde

    Insert

    HseIF4AI (EIF4A1 Human)
    

    Expression

    Insect

    Mutation

    3 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-Cas9-T2A-TdT

    Plasmid

    #126424

    Purpose

    Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCI-neo beta catenin S33Y

    Plasmid

    #16519

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    beta catenin (CTNNB1 Human)
    

    Expression

    Mammalian

    Mutation

    S33Y (SW48 mutation)

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFG4

    Plasmid

    #87251

    Purpose

    gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Hyg
    

    Tags

    parS segments

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge