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Omega 1

Plasmid

#118046

Purpose

GoldenBraid2.0 Omega 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Expression

Bacterial, Insect, and Mamm…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDC216

Plasmid

#115791

Purpose

Expresses rat Syntaxin 1

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

Syntaxin1 (Stx1a Rat)

Tags

hexa histidine

Expression

Bacterial

Mutation

C145S S193C C271S C272S

Promoter

T7

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCX-ThPDE5VV

Plasmid

#134778

Purpose

cGMP FRET biosensor

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

ThPDE5VV

Tags

cpVenus-Venus and mTurquoise2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PP1 7-300

Plasmid

#26566

Depositor

Wolfgang Peti

Article

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

PP1a (PPP1CA Human)

Tags

His6, TEV cleavage site, and Thio-6

Expression

Bacterial

Mutation

Contains only aa 7-330

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
pAtetO_6xHis-Bst-LF

Plasmid

#145799

Purpose

Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

Depositor

Andrew Ellington

Article

Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

Insert

Bst-LF

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Halo-TNFa-RUSH

Plasmid

#166901

Purpose

RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-Halo

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

HaloTag

Expression

Mammalian

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NAD-Snifit

Plasmid

#113658

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CRY2low-tdTomato

Plasmid

#104067

Purpose

Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2low-tdTomato

Expression

Mammalian

Mutation

CRY2 aa 1-491 truncation, R489E A491D

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-CaM-NanoLuc-15aa-uTEVp

Plasmid

#163034

Purpose

FLiCRE protease with luciferin-gating for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-NanoLuc-15aa-uTEVp

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Active

Plasmid

#173072

Purpose

Active SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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p131CB10-tphKAB

Plasmid

#241911

Purpose

TPA catabolic operon under the control of a PCA inducible biosensor

Depositor

Neil Dixon

Article

Chacon et al Trends Biotechnol. 2025 Mar;43(3):647-67

Insert

tphK, tphA2, tphA3, tphB, tphA1

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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TL1 DdCBE A1 (DddA6-C)

Plasmid

#221992

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: T1413I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TL1 DdCBE T2 (DddA6-N)

Plasmid

#221993

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: Q1310R, S1330I, T1380I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209726

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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dGFP-Rab27a

Plasmid

#170862

Purpose

Mammalian expression of dGFP-Rab27a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-27a (RAB27A Human)

Tags

dEGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCX-SponGee

Plasmid

#134775

Purpose

mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

SponGee

Tags

FLAG and mRFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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NylCp2-TS_pET28a

Plasmid

#215421

Purpose

Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2-TS

Expression

Bacterial

Mutation

D122G/H130Y/D36A/E263Q

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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ss-SigmaR1DTM-mNeonGreen

Plasmid

#226575

Purpose

Encodes mutant SigmaR1 protein (transmembrane region deletion) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_ATP1.0mut

Plasmid

#167578

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin WT

Plasmid

#50853

Purpose

expresses human cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Omega 2

Plasmid

#118047

Purpose

GoldenBraid2.0 Omega 2 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Expression

Bacterial, Insect, and Mamm…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Alpha2

Plasmid

#118045

Purpose

GoldenBraid2.0 Alpha 2 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Expression

Bacterial, Insect, and Mamm…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

Plasmid

#233445

Purpose

PiggyBac donor plasmid to overexpress a full length ADAR2

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHH0103_PFN1_Profilin

Plasmid

#110028

Purpose

Protein expression and purification of PFN1_Profilin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PFN1_Profilin (PFN1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Lyric-mCherry

Plasmid

#226563

Purpose

Encodes Lyric protein labeled with mCherry

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lyric (Lysine-rich CEACAM1 coisolated) (MTDH Human)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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BB2_AB

Plasmid

#98534

Purpose

empty BB2 for transcription unit cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KB

Plasmid

#139310

Purpose

Recruitable PI4KB

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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Lyric-mNeonGreen

Plasmid

#226562

Purpose

Encodes Lyric protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lyric (Lysine-rich CEACAM1 coisolated) (MTDH Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-6h-GST-TEVp

Plasmid

#172887

Purpose

Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Insert

TEV protease

Tags

GST solubility tag and HHHHHH

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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