19,660 results

1. pAAV.hSyn.iGluSnFR3.v857.GPI

   Plasmid

   #178331

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Has Service

   AAV1

   Insert

   pAAV hSyn iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI Anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFGAMS-mEos2

   Plasmid

   #105128

   Purpose

   Expresses human FGAMS-mEos2 fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   French et al Science. 2016 Feb 12;351(6274):733-7. do

   Insert

   PFAS (PFAS Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX-TRV1

   Plasmid

   #180515

   Purpose

   Agrobacterium tumefaciens-based expression of TRV1

   Depositor

   Jose-Antonio Daros

   Article

   Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

   Insert

   Tobacco rattle virus RNA1
   

   Expression

   Plant

   Promoter

   35S CaMV

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOsTIR1w/oGFP

   Plasmid

   #102883

   Purpose

   Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   OsTIR1
   

   Tags

   3x myc

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJ404-DogCatcher-sfGFP

   Plasmid

   #171930

   Purpose

   Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher-sfGFP
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

   Plasmid

   #89609

   Purpose

   over-expression of VEGF-A(165) in human cells

   Depositor

   Fernando Fierro

   Insert

   VEGF-A(165) (VEGFA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K188E

   Promoter

   MNDU3

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV_NLS-dSaCas9-NLS-VPR

   Plasmid

   #68495

   Purpose

   AAV vector containing nuclease null SaCas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   dSaCas9
   

   Use

   AAV

   Tags

   VPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-luc-miR21(P)

   Plasmid

   #20382

   Depositor

   Bryan Cullen

   Article

   Zeng et al RNA. 2003 Jan . 9(1):112-23.

   Insert

   mir21(P) targets
   

   Expression

   Mammalian

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pC1-CMV-mito-DAAO

   Plasmid

   #141132

   Purpose

   Expresses DAAO with mitochondrial localization signal in mammalian cells

   Depositor

   Vsevolod Belousov

   Insert

   mito-DAAO
   

   Tags

   2 mitochondrial localization signals and 7xHis

   Expression

   Mammalian

   Mutation

   deleted DAAO amino acids 367-368

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPDK3876(TRV-RNA2-pPEBV-MCS)

    Plasmid

    #149275

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter
    

    Use

    T-dna vector

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGfaABC1D-nLac

    Plasmid

    #53131

    Purpose

    expression of lacZ driven by human GfaABC1D promoter

    Depositor

    Michael Brenner

    Article

    Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains ABC1D regions of Gfa2 promoter, -1757 to…

    Promoter

    human Gfa2

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

    Plasmid

    #133028

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Golgin84 (GOLGA5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-673

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. IRE1 alpha-pcDNA3.EGFP

    Plasmid

    #13009

    Depositor

    Fumihiko Urano

    Article

    Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

    Insert

    Inositol Requiring Enzyme-1 (ERN1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. TriEx-4 DHX36

    Plasmid

    #68368

    Purpose

    Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

    Depositor

    James Vaughn

    Article

    Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    Histidine

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV ie enhancer/promoter

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

15. DUSP6

    Plasmid

    #27975

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. TS module

    Plasmid

    #26021

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Tension Sensor module
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-nCas9n-nanos 3’UTR

    Plasmid

    #62542

    Purpose

    expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

    Insert

    nanos 3' UTR (nanos3 Zebrafish)
    

    Use

    CRISPR

    Mutation

    3' UTR only

    Promoter

    SP6

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIP-CMV-mCherry-53BP1

    Plasmid

    #127658

    Purpose

    Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Syn-ChrimsonR-GFP

    Plasmid

    #58806

    Purpose

    AAV expression of ChrimsonR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1 gfaABC1D-cyto-GCaMP6f

    Plasmid

    #52925

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Baljit Khakh

    Article

    Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

    Has Service

    AAV5

    Insert

    GCaMP6f
    

    Use

    AAV

    Available Since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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