We narrowed to 17,152 results for: IGH@;

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CRISPR-StAR2 (Retro)

Plasmid

#222688

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR3 (Retro)

Plasmid

#222689

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct2

Plasmid

#28195

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa86-414 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro

Plasmid

#209646

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-Q2L7-T2176C

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct8

Plasmid

#28201

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa275-313 deleted

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pETNKI-strepII-3C-LIC-Amp

Plasmid

#108707

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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dxCas(3.6)-VPR

Plasmid

#108388

Purpose

Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

dxCas(3.6)-VPR

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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ELK1_pet28a

Plasmid

#131647

Purpose

Bacterial expression of ETS gene ELK1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK1 (ELK1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pProEx M-Ras(G22C) CL

Plasmid

#224268

Purpose

Protein purification of M-Ras (G22C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

M-Ras (MRAS Human)

Expression

Bacterial

Mutation

G22C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pProEx Rit1(G47C) CL

Plasmid

#224267

Purpose

Protein purification of Rit1 (G47C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rit1 (RIT1 Human)

Expression

Bacterial

Mutation

G47C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pProEx Ypt1(S17C) CL

Plasmid

#224265

Purpose

Protein purification of Ypt1 (S17C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Ypt1 (YPT1 Budding Yeast)

Expression

Bacterial

Mutation

S17C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pJT121_GalL_nCDA1-VQRBE3

Plasmid

#145086

Purpose

Expressing base editor nCDA1-VQRBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-VQRBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A, D1135V, R1335Q, T1337R)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7REwt

Plasmid

#213935

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Mutation

AO7 cloned into the first multiple cloning site o…

Promoter

T7 promoter-1

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct5

Plasmid

#28198

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa257-414 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
B2396_BTB-EGFP

Plasmid

#215099

Purpose

Expresses BCL6 BTB(1-129)-EGFP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-EGFP (BCL6 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-haA3A-CBE-G-C

Plasmid

#220899

Purpose

AAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNA

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-C

Use

AAV

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BmNG

Plasmid

#232207

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-PmNG

Plasmid

#232201

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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ETV2_pet28a

Plasmid

#131665

Purpose

Bacterial expression of ETS gene ETV2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV2 (ETV2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluu

Plasmid

#106122

Purpose

Fast indicator for synaptic glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

synapsin

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBC2-null

Plasmid

#162749

Purpose

MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MBC1-null

Plasmid

#162748

Purpose

MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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BE3-Gam

Plasmid

#100807

Purpose

Expresses BE3-Gam in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

BE3-Gam

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAM4941 (pCV0053)

Plasmid

#120083

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-PmSc

Plasmid

#232549

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Mutation

None

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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TRE3G-CTCF-dRBR-HA-MND-zeo

Plasmid

#232802

Purpose

Inducible over expression of RNA-binding domain deficient form of CTCF.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

Ctcf-dRBR (Ctcf Mouse)

Use

Lentiviral

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

Plasmid

#193854

Purpose

Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

5' Cbr-unc-119(+) + 5' mNeonGreen

Use

CRISPR and Cre/Lox

Expression

Worm

Promoter

Cbr-unc-119p

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pProEx Rheb(R15C) CL

Plasmid

#224266

Purpose

Protein purification of Rheb (R15C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rheb (RHEB Human)

Expression

Bacterial

Mutation

R15C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

Plasmid

#159283

Purpose

A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Inserts

Human INS sgRNA
Human INS guide RNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-CAAX

Plasmid

#51567

Purpose

Constitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

CAAX and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only

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