We narrowed to 12,778 results for: NUC

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NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1

Plasmid

#183400

Purpose

Dual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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1137=Hsp70Bb-Cas9[dsRed]

Plasmid

#149424

Purpose

The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

Cas9

Use

CRISPR

Tags

eGFP

Expression

Insect

Promoter

Dmel Hsp70Bb

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV- CAG-h2BmRuby2FLAG

Plasmid

#116872

Purpose

Nuclear localized mRuby2FLAG

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Insert

H2BmRuby2FLAG

Use

AAV

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pH2B-SCFP1-2A-mTq2-NES

Plasmid

#60491

Purpose

Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm

Depositor

Dorus Gadella

Article

Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

Inserts

H2B-SCFP1
mTurquoise2-NES

Tags

SCFP1 and mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pDGE474

Plasmid

#153265

Purpose

M1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE475

Plasmid

#153266

Purpose

M2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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T7-RelA(S276E)

Plasmid

#24154

Depositor

Warner Greene

Article

Chen et al Mol Cell Biol. 2005 Sep . 25(18):7966-75

Insert

RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

S276E

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
mPapaya-Nup50-N-10

Plasmid

#56348

Purpose

Localization: Nuclear Pore, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Nup50 (NUP50 Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPlum-CENPB-N-22

Plasmid

#55957

Purpose

Localization: Nucleus/Centromeres, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

CENPB (CENPB Human)

Tags

mPlum

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-CENPA

Plasmid

#57359

Purpose

Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CENPA (CENPA Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1

Plasmid

#183402

Purpose

Dual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCS-nlstdTomato

Plasmid

#132985

Purpose

full length of nuclear localized tdTomato in pCS expression vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

tdTomato

Tags

NLS and myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMEH280 NLS dTomato 3x high affinity MS2 loops

Plasmid

#168211

Purpose

Mammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loops

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pgRNA-backbone

Plasmid

#172517

Purpose

Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

Depositor

Leonid Kruglyak

Article

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

gRNA cassette

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBS/KS-mId3sp

Plasmid

#16045

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id3 (Id3 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 365-635

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
H2A-mTurquoise-RHOG-QL-deltaCAAX

Plasmid

#176096

Purpose

Constitutively active, nuclear localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Expression

Mammalian

Mutation

QL

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM3-N-22

Plasmid

#54166

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM3

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCngA2-ISH

Plasmid

#15855

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Cyclic Nucleotide Gated Channel Alpha 2 (Cnga2 Mouse)

Use

Cloning vector

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pJG380

Plasmid

#91188

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9+gNt_R2+donor

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pKL1-222

Plasmid

#219409

Purpose

Nuclear expression of Synrophin PDZ clamp protein

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

pTEF1-NLS-(Syntrophin-PDZ)x4-tADH1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-NLS-ZapCmR2

Plasmid

#59012

Purpose

Nuclear zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NLS-ZapCmR2 genetically encoded Zn(II) sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR2

Plasmid

#59015

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
FCIV1-GR

Plasmid

#72701

Purpose

Lentiviral expression of rat GR (2nd generation)

Depositor

Freddy Jeanneteau

Article

Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
mEos2-Fibrillarin-7

Plasmid

#57377

Purpose

Localization: Nucleoli, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Fibrillarin (FBL Human)

Tags

mEos2

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mOrange2-H2B-6

Plasmid

#57962

Purpose

Localization: Nucleus/Histones, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Insert

H2B (H2BC11 Human)

Tags

mOrange2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
mEmerald-CENPB-N-22

Plasmid

#54037

Purpose

Localization: Nucleus/Centromeres, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

CENPB (CENPB Human)

Tags

mEmerald

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pRS403red-luxTA2-4F

Plasmid

#166030

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405red-phlTA2-1E

Plasmid

#166029

Purpose

Expresses phlTA(2-1E) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

phlF

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

Promoter

pPGK1 mutant

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGK415-luxTA2-4F

Plasmid

#165971

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pgRNA-guideless

Plasmid

#129464

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Guideless gRNA backbone

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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