We narrowed to 23,290 results for: CRISPR

1. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

   Plasmid

   #87350

   Purpose

   S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/S355C/C574S/S867C

   Promoter

   T7

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB6627

   Plasmid

   #106159

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. psAAVS1-T2

   Plasmid

   #60543

   Purpose

   GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

   Depositor

   William Simonds

   Article

   Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

   Insert

   ProtoSpacer AAVS1-T2 (AAVS1 Human)
   

   Use

   CRISPR; Kan

   Expression

   Mammalian

   Available Since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM423

   Plasmid

   #172408

   Purpose

   Expresses sfGFP (1-10) and MCP-GFP11

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   MCP, bipartite sfGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   sfGFP {1-10}

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS416gT-GalL-Cas9

   Plasmid

   #79903

   Purpose

   pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

   Depositor

   Ronald Davis

   Article

   Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

   Inserts

   Cas9
   guide RNA (gRNA)
   Tetracycline Repressor
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   GPM1 promoter, GalL, and RPR1 promoter with TetO …

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXGFPC-5 Pxyl-dcas9 (Spa)

   Plasmid

   #133318

   Purpose

   expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus pasteurianus)
   

   Use

   Unspecified

   Promoter

   xylose

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pV1435

   Plasmid

   #111439

   Purpose

   Solo vector pV1382 + sgCgADE2

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgCgADE2
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCK760

   Plasmid

   #192643

   Purpose

   Expresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   BBa_J23110-sfGFP
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   BBa_J23110

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV/3' Box_(GLuc)_TOP2

   Plasmid

   #68435

   Purpose

   Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP2 construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6_(GLuc)_INT[Spinach2]

    Plasmid

    #68430

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the "Spinach2" aptamer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcF-1

    Plasmid

    #131023

    Purpose

    Single spacer array with spacer #1 for FnCas12a.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Insert

    single spacer array for FnCas12a
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23119

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. EFS eGFP hPGK BSD

    Plasmid

    #188520

    Purpose

    Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTZ tRNA_gRNA empty scaffold

    Plasmid

    #188450

    Purpose

    Expression of gRNAs using a human tRNA promoter

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    tRNA glutamine

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK503

    Plasmid

    #155380

    Purpose

    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    May 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192683

    Purpose

    Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

    Plasmid

    #133342

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK671.306

    Plasmid

    #192641

    Purpose

    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    BBa_J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5

    Plasmid

    #61256

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFETCh_NFIA_iso1

    Plasmid

    #104039

    Purpose

    Donor vector for 3' FLAG tag of human NFIA_iso1

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NFIA_iso1 homology arms (NFIA Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mammalian

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV/MASC_(GLuc)_INT

    Plasmid

    #68440

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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