We narrowed to 18,729 results for: Mut

Showing: 7301 - 7320 of 18729 results

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pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

Plasmid

#226101

Purpose

HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

HRI (EIF2AK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

SIFI degron helices 1 & 2 deleted (Δ61-112)

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_SRGAP2A_SH3(W765A)_GFP

Plasmid

#222616

Purpose

Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

Depositor

Franck Polleux

Article

Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

Insert

SRGAP2 (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Point mutation W765A (SH3 domain)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_SRGAP2A_SH3(W765A)_HA

Plasmid

#222614

Purpose

Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

Depositor

Franck Polleux

Article

Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

Insert

SRGAP2 (SRGAP2 Human)

Tags

HA

Expression

Mammalian

Mutation

Point mutation W765A (SH3 domain)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG SRGAP2A_EVH1(P339L F342C)

Plasmid

#222613

Purpose

Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

Depositor

Franck Polleux

Article

Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

Insert

SRGAP2 (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Point mutations P339L and F342C

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-1 D141N

Plasmid

#222654

Purpose

Mammalian expression vector to express mouse Regnase-1 D141N mutant

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 D252N

Plasmid

#222652

Purpose

For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcs2-N-HRI(1-138)-GFP-IRES-mCherry

Plasmid

#226100

Purpose

HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

HRI (EIF2AK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

contains only N-HRI (aa 1-138)

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*/PBM*

Plasmid

#195221

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

Dual mutant for HRS>AAA / lacking DTHL

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pACB120

Plasmid

#222213

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA(K10N, S11R, W15C, G76V, G258D)

Tags

6x His

Expression

Bacterial

Mutation

Contains five amino acid mutations: K10N, S11R, W…

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-puro_Panc480-MT7

Plasmid

#200941

Purpose

Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

multiplexed sgRNA array

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6, EF-1a

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-VcnTS-E1015A-E1021A

Plasmid

#213411

Purpose

Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.

Depositor

Brenton Hoffman

Article

Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

Insert

VcnTS-E1015A-E1021A (VCL Chicken, Synthetic)

Use

Lentiviral

Mutation

Mutated vinculin glutamic acid 1015 and 1021 to a…

Promoter

CMV

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61A)(HA)

Plasmid

#195025

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61A mutation

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)

Use

Gateway cloning

Tags

3xHA

Mutation

T59/T61A

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61D)(HA)

Plasmid

#195026

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)

Use

Gateway cloning

Tags

3xHA

Mutation

T59/T61D

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115G)-Talin rod-mCherry

Plasmid

#210026

Purpose

Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry

Expression

Mammalian

Mutation

SpyTag003 M115G mutation to modify SpyCatcher003 …

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003(M115A)-Talin rod-mCherry

Plasmid

#210025

Purpose

Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry

Expression

Mammalian

Mutation

SpyTag003 M115A mutation to modify SpyCatcher003 …

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003(V114T, V116T)-Talin rod-mCherry

Plasmid

#210027

Purpose

Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry

Expression

Mammalian

Mutation

SpyTag003 V114T and V116T mutations to modify Spy…

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S172A_M173A RAD23A

Plasmid

#201574

Purpose

Expresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutations S172A and M173A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V195A RAD23A

Plasmid

#201575

Purpose

Expresses a variant of human RAD23A containing mutation V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V195A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_C344A RAD23A

Plasmid

#201577

Purpose

Expresses a variant of human RAD23A containing mutation C344A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation C344A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_F354A RAD23A

Plasmid

#201578

Purpose

Expresses a variant of human RAD23A containing mutation F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation F354A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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