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Plasmid#62017Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertMETTL7A (METTL7A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA
Plasmid#205316PurposeAll-in-one AAV vector expressing ISAam1 TnpB and its reRNADepositorInserthumanized ISAam1 TnpB
UseAAVTags3 x HAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX311 Luciferase
Plasmid#117735PurposeOpen reading frame vector encoding LuciferaseDepositorInsertfirefly Luciferase
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-mTagBFP2-B19G
Plasmid#100799Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTADepositorHas ServiceAAV1InsertmTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
UseAAVTagsExpressionMammalianMutationPromoterTREtightAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
Plasmid#128424PurposeHuman Codon Optimized dCas9-DNMT3A3LDepositorInsertdCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterT7 PromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-ER-LAR-GECO1
Plasmid#61244PurposeExpresses LAR-GECO1 in the endoplasmic reticulum in mammalian cellsDepositorInsertLAR-GECO1
UseTagsER-retention sequence: KDEL and ER-targeting sequ…ExpressionMammalianMutationSubstitutions relative to R-GECO1: V51W/I113V/N35…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-Thy1.1 DEST
Plasmid#17442PurposeDestination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reactionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
ATG-2460
Plasmid#108713PurposeEF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)DepositorInsertCBR2opt-copGFP fusion
UseLentiviralTagsCBR2opt fused to copGFPExpressionMutationPromega used directed evolution to create CBR (Cl…Promoterelongation factor-1Available sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN1-GCaMP6m-XC
Plasmid#111543PurposeImproved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-XDepositorInsertGCaMP6m-XC
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only