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SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Publication

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Expression

Mammalian

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pICH47822

Plasmid

#48009

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pCC1-4k-BBclone2

Plasmid

#137070

Purpose

E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

Depositor

Debra Hansen

Publication

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Inserts

BB0323
P13
DipA
Lmp1

Expression

Bacterial

Mutation

wild-type, full-length (signal-peptide-containing…

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTW335h3

Plasmid

#237326

Purpose

expresses O. sativa RbcL RbcS-6xHis

Depositor

Tina Wang

Publication

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Inserts

O. sativa RbcL
O. sativa RbcS-6xHis

Tags

6xHis and None

Expression

Bacterial

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-puro

Plasmid

#208032

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Publication

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->sGFP-P2A-mCherry

Plasmid

#162232

Purpose

Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP and mCherry

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pB-CAG-TEVp-IRES-mCherry

Plasmid

#174377

Purpose

For making TEVP cell line with hypBase

Depositor

Ian Wickersham

Publication

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

TEV Protease

Expression

Mammalian

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NK803

Plasmid

#166057

Purpose

Puromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta

Depositor

Nicole King

Publication

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Inserts

Pac puromycin resistance gene
LifeAct-mCherry

Use

S. rosetta expression

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE503

Plasmid

#153276

Purpose

Intermediate sgRNA array cloning vector position 1

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE504

Plasmid

#153277

Purpose

Intermediate sgRNA array cloning vector position 2

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLITMUS*-PrpoN-Cro-PBBa_J23106-geneIII

Plasmid

#134353

Purpose

Phagemid

Depositor

Mark Isalan

Publication

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Cro

Expression

Bacterial

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3E-FKBP-HA no-pA

Plasmid

#82598

Purpose

HA epitope tag fused to C-terminus of human FK506 binding protein 12 (FKBP12) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FKBP-HA (no pA)

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGAPTrap-GFP

Plasmid

#82506

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

Depositor

Ed Stanley

Publication

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD1-GFP

Plasmid

#80649

Purpose

Promoter1 with BCD1-gfp

Depositor

Nathan Hillson

Publication

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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ACV

Plasmid

#27791

Depositor

Steven Vogel

Publication

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Amber-Cerulean-Venus

Use

Stoichiometry standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
pRSET-WHaloCaMP1a-EGFP

Plasmid

#205303

Purpose

Expression of WHaloCaMP1a-EGFP fusion in E. coli

Depositor

Eric Schreiter

Insert

WHaloCaMP1a-EGFP

Expression

Bacterial

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDGE508

Plasmid

#153280

Purpose

End-linker for cloning 2 sgRNA arrays into any recipient vector

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

linker

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJMD14

Plasmid

#138654

Purpose

phiOZJ integrating Streptomyces expression vector, intact RDF

Depositor

Joshua Blodgett

Publication

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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Gq sensor

Plasmid

#137810

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid

Depositor

Dorus Gadella

Publication

Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

Insert

Gq sensor

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pADH140

Plasmid

#90988

Purpose

NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

Depositor

Aaron Hernday

Publication

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Inserts

C. maltosa LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2

Use

CRISPR

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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