We narrowed to 8,596 results for: Set

1. pcU6_3 sgRNA

   Plasmid

   #92359

   Purpose

   Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.3 promoter and gRNA cloning cassette
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.3

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-pur-CAG-mCherry

   Plasmid

   #159458

   Purpose

   AAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection gene

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   mCherry
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pv6_CBX7_Chromo_TAF3_Phd

   Plasmid

   #179400

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_Chromo - TAF3_Phd
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

   Plasmid

   #176628

   Purpose

   TF A self-activation cassette in MultiFate-2.3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZac2.1 U6-SaAi9L-U6-SaAi9R

   Plasmid

   #78604

   Purpose

   AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   gRNAs for SaCas9 targeting Ai9 locus
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

6. AAVS1-Blasticidin-CAG-Flpe-ERT2

   Plasmid

   #68461

   Purpose

   AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   mammalian-codon-optimized Flpe recombinase fused with ERT2
   

   Use

   Synthetic Biology

   Tags

   ERT2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-AtMIR390a-B/c

   Plasmid

   #51778

   Purpose

   Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtMIR390a-B/c
   

   Use

   Gateway entry vector

   Mutation

   A. thaliana MIR390a precursor sequence including …

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

   Plasmid

   #218513

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of the Plac_mrfp cassette

   Promoter

   PgyrA_ermBL-ermB'

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-phiC31-LP-TC

   Plasmid

   #183755

   Purpose

   AAVS1 targeting construct with the phiC31 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   FRT-mCherry-attP-BleoR-F3
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAT-PB-CG2APtk

    Plasmid

    #86003

    Purpose

    homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Insert

    eGFP_T2A_puro_dTK
    

    Use

    Piggybac exciseable

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLJ-F2P-LoxP-PGK-NeoR-LoxP

    Plasmid

    #227503

    Purpose

    Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    Prdm8 promoter
    

    Use

    AAV and Cre/Lox

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW212

    Plasmid

    #172196

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Expression

    Yeast

    Promoter

    10B2 (P1 specific promoter)

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-mGLIS1

    Plasmid

    #33273

    Purpose

    Retroviral expression of mouse Glis1

    Depositor

    Shinya Yamanaka

    Article

    Maekawa et al Nature. 2011 . 474(7350):225-9.

    Insert

    GLIS1, Gateway casette A (Glis1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC-GFP-HS

    Plasmid

    #133305

    Purpose

    "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    hok/sok toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFlp-Gm

    Plasmid

    #222335

    Purpose

    Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Promoter

    cl, flp, and sacB

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200403

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid

    #176624

    Purpose

    TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA

    Plasmid

    #176629

    Purpose

    TF C self-activation cassette in MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AWP-029

    Plasmid

    #213966

    Purpose

    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.

    Depositor

    Alexander Westermann

    Article

    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

    Insert

    Cas12a (cas12a Segatella bryantii, Synthetic)
    

    Tags

    3XFLAG

    Expression

    Bacterial

    Mutation

    D875A to inactivate target nuclease activity

    Promoter

    PcfxA, IPTG inducible

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC8_MF (VE5596)

    Plasmid

    #139768

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Type

    Empty backbone

    Promoter

    PH or p10

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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