Addgene

We narrowed to 1,532 results for: Tef;

Showing: 1081 - 1110 of 1532 results

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PP164

Plasmid

#78988

Purpose

Supplemental Figure 4. Promoter short ppTEF1. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCSP1-HA-xEcto

Plasmid

#20900

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (trim33 Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

contains full unsequenced 3' UTR

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
I766T USP7 UBL-12

Plasmid

#227232

Purpose

Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

I766T; aa 535-775

Promoter

T7-promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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L757P USP7 UBL-12

Plasmid

#227231

Purpose

Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

L757P; aa 535-775

Promoter

T7-promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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R152C USP7 TRAF

Plasmid

#227229

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

R152C; aa 62-205

Promoter

T7-promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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G392A FL USP7

Plasmid

#227217

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

G392A

Promoter

T7-promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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N418S FL USP7

Plasmid

#227215

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

N418S

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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H111Q USP7 TRAF

Plasmid

#227227

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

H111Q; aa 62-205

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
G392D FL USP7

Plasmid

#227216

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

G392D

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
K420E FL USP7

Plasmid

#227223

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

K420E

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L373F FL USP7

Plasmid

#227220

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

L373F

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Y331H FL USP7

Plasmid

#227218

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

Y331H

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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P273H FL USP7

Plasmid

#227221

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

P273H

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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M225I FL USP7

Plasmid

#227219

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

M225I

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
F118L USP7 TRAF

Plasmid

#227228

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

F118L; aa 62-205

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L469R FL USP7

Plasmid

#227225

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

L469R

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E345K FL USP7

Plasmid

#227224

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

E345K

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
V485G FL USP7

Plasmid

#227222

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

V485G

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (WT)

Plasmid

#197001

Purpose

Mammalian inducible expression construct of myc-tagged SPOP

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

-

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046243

Plasmid

#149143

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046240

Plasmid

#149144

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046547

Plasmid

#149145

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046253

Plasmid

#149135

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046557

Plasmid

#149131

Purpose

Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046543

Plasmid

#149134

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046316

Plasmid

#149125

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046542

Plasmid

#149122

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046191

Plasmid

#149115

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046469

Plasmid

#149116

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046565

Plasmid

#149097

Purpose

Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge