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We narrowed to 5,542 results for: SPI

Showing: 1441 - 1480 of 5542 results

  1. pcDNA3.3_SARS2_JN.1

    Plasmid
    #218678
    Purpose
    expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (JN.1)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L455S on the basis of BA.2.86
    Promoter
    CMV
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    218678-plasmid-map-sequence-id-437262

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  2. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid
    #92303
    Purpose
    Expression of humanized TsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huTsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92303-plasmid-map-sequence-id-181636

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  3. C22m

    Plasmid
    #120963
    Purpose
    MoClo golden gate assembly CD part for mSpinach (fluorescent RNA aptamer for DFHBI, suitable for in vivo use; see DOI: 10.1021/sb400089c). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mSpinach
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120963-plasmid-map-sequence-id-236357

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  4. pcDNA3.3_SARS2_BA.2.87.1

    Plasmid
    #218679
    Purpose
    expressing SARS-CoV-2 BA.2.87.1 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (BA.2.87.1)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    218679-plasmid-map-sequence-id-437265

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  5. MERS_NSP13_BV

    Plasmid
    #234378
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp13_MERS
    Use
    Baculovirus expresssion
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234378-plasmid-map-sequence-id-469466

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  6. hIgG1-F1-Fc

    Plasmid
    #194597
    Purpose
    Mammalian expression of F1 nanobody upstream of human IgG1; F1 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike
    Depositor
    Ben Murrell
    Insert
    F1-Fc (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194597-plasmid-map-sequence-id-382112

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  7. hIgG1-E2-Fc

    Plasmid
    #194595
    Purpose
    Mammalian expression of E2 nanobody upstream of human IgG1; E2 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike
    Depositor
    Ben Murrell
    Insert
    E2-Fc (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194595-plasmid-map-sequence-id-381544

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  8. hIgG1-G6-Fc

    Plasmid
    #194594
    Purpose
    Mammalian expression of G6 nanobody upstream of human IgG1; G6 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike
    Depositor
    Ben Murrell
    Insert
    G6-Fc (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194594-plasmid-map-sequence-id-381539

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  9. hIgG1-C7-Fc

    Plasmid
    #194596
    Purpose
    Mammalian expression of C7 nanobody upstream of human IgG1; C7 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike
    Depositor
    Ben Murrell
    Insert
    C7-Fc (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    194596-plasmid-map-sequence-id-381540

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  10. SARS2nsp3_MacroD_ECBio2

    Plasmid
    #234388
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp3_SARS2
    Use
    Tags
    LVPRGSSSKGGYGLNDIFEAQKIEWHE and MHHHHHHSSGVDLGTEN…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234388-plasmid-map-sequence-id-470057

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  11. nsp14_EC_Bio1

    Plasmid
    #234376
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp14_SARS2
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    234376-plasmid-map-sequence-id-469875

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  12. SARS2nsp3_MacroD_EC

    Plasmid
    #234389
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp3_SARS2
    Use
    Tags
    MHHHHHHSSGVDLGTENLYFQSM
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    234389-plasmid-map-sequence-id-469871

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  13. phCMV-229E-D15

    Plasmid
    #188908
    Purpose
    express truncated 229E spike for pseudovirus production
    Depositor
    David Nemazee
    Insert
    229E spike (S 229E)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    c-terminal 15aa deletion
    Promoter
    CMV
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188908-plasmid-map-sequence-id-370665

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  14. pcDNA3.3_SARS2_XBB.1.5

    Plasmid
    #196585
    Purpose
    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.5)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G252V, F486P on the basis of XBB
    Promoter
    CMV
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196585-plasmid-map-sequence-id-386779

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  15. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

    Plasmid
    #160629
    Purpose
    TU for the expression of LbCas12a
    Depositor
    Diego Orzaez
    Insert
    AtUBQ10-:LbCpf1(Cas12a):Tnos
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    UBQ10
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160629-plasmid-map-sequence-id-321990

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  16. pET24a MDH_AQUAR

    Plasmid
    #204239
    Purpose
    Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_AQUAR
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204239-plasmid-map-sequence-id-413339

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  19. pAIP4_6WEY

    Plasmid
    #234234
    Purpose
    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234234-plasmid-map-sequence-id-464198

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  20. pAIP4_5HIH

    Plasmid
    #234205
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234205-plasmid-map-sequence-id-464170

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  21. pAIP4_2H2Z

    Plasmid
    #234147
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234147-plasmid-map-sequence-id-464113

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  26. SARS2_HisSUMOTEV-nsp7/nsp8

    Plasmid
    #234405
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Inserts
    Nsp7_SARS2
    Nsp8_SARS2
    Use
    Tags
    His6-SUMO-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234405-plasmid-map-sequence-id-469465

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  27. NSP12:4393-5324_Nbio_Chis_BV

    Plasmid
    #234377
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp12_SARS2
    Use
    Baculovirus expresssion
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234377-plasmid-map-sequence-id-469461

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  28. NSP13:5325-5925_BVnAvi

    Plasmid
    #234375
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp13_SARS2
    Use
    Baculovirus expresssion
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234375-plasmid-map-sequence-id-469460

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  29. SARS1_NSP13_BV_NBIO-TEV_CTHROM-his

    Plasmid
    #234382
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp13_SARS
    Use
    Baculovirus expresssion
    Tags
    LVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234382-plasmid-map-sequence-id-469458

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  32. NSP13:5325-5925_BV

    Plasmid
    #234374
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp13_SARS2
    Use
    Baculovirus expresssion
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234374-plasmid-map-sequence-id-469457

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Showing: 1441 - 1480 of 5542 results