We narrowed to 5,543 results for: SPI

1. MERS_NSP13_BV_BIO

   Plasmid

   #234379

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp13_MERS
   

   Use

   Baculovirus expresssion

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.3_SARS2_JN.1

   Plasmid

   #218678

   Purpose

   expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (JN.1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L455S on the basis of BA.2.86

   Promoter

   CMV

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY127 (pcDNA3.1-U6-huTsCpf1)

   Plasmid

   #92303

   Purpose

   Expression of humanized TsCpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huTsCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C22m

   Plasmid

   #120963

   Purpose

   MoClo golden gate assembly CD part for mSpinach (fluorescent RNA aptamer for DFHBI, suitable for in vivo use; see DOI: 10.1021/sb400089c). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   mSpinach
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.3_SARS2_BA.2.87.1

   Plasmid

   #218679

   Purpose

   expressing SARS-CoV-2 BA.2.87.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (BA.2.87.1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MERS_NSP13_BV

   Plasmid

   #234378

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp13_MERS
   

   Use

   Baculovirus expresssion

   Tags

   MHHHHHHSSGRENLYFQG

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hIgG1-F1-Fc

   Plasmid

   #194597

   Purpose

   Mammalian expression of F1 nanobody upstream of human IgG1; F1 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Ben Murrell

   Insert

   F1-Fc (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hIgG1-E2-Fc

   Plasmid

   #194595

   Purpose

   Mammalian expression of E2 nanobody upstream of human IgG1; E2 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Ben Murrell

   Insert

   E2-Fc (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hIgG1-G6-Fc

   Plasmid

   #194594

   Purpose

   Mammalian expression of G6 nanobody upstream of human IgG1; G6 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Ben Murrell

   Insert

   G6-Fc (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hIgG1-C7-Fc

    Plasmid

    #194596

    Purpose

    Mammalian expression of C7 nanobody upstream of human IgG1; C7 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Ben Murrell

    Insert

    C7-Fc (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SARS2nsp3_MacroD_ECBio2

    Plasmid

    #234388

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp3_SARS2
    

    Use

    Tags

    LVPRGSSSKGGYGLNDIFEAQKIEWHE and MHHHHHHSSGVDLGTEN…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. nsp14_EC_Bio1

    Plasmid

    #234376

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp14_SARS2
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SARS2nsp3_MacroD_EC

    Plasmid

    #234389

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp3_SARS2
    

    Use

    Tags

    MHHHHHHSSGVDLGTENLYFQSM

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. phCMV-229E-D15

    Plasmid

    #188908

    Purpose

    express truncated 229E spike for pseudovirus production

    Depositor

    David Nemazee

    Insert

    229E spike (S 229E)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c-terminal 15aa deletion

    Promoter

    CMV

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.3_SARS2_XBB.1.5

    Plasmid

    #196585

    Purpose

    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XBB.1.5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G252V, F486P on the basis of XBB

    Promoter

    CMV

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

    Plasmid

    #160629

    Purpose

    TU for the expression of LbCas12a

    Depositor

    Diego Orzaez

    Insert

    AtUBQ10-:LbCpf1(Cas12a):Tnos
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    UBQ10

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET24a MDH_AQUAR

    Plasmid

    #204239

    Purpose

    Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_AQUAR
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p112A1NE

    Plasmid

    #85983

    Purpose

    For construction of cDNA expression library.

    Depositor

    Wolf Frommer

    Article

    Riesmeier et al EMBO J. 1992 Dec;11(13):4705-13.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Adh1

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-EgC*

    Plasmid

    #20378

    Depositor

    Andrew Lee

    Article

    Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

    Insert

    Eglin C wt*
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F10W

    Promoter

    Available since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAIP4_6WEY

    Plasmid

    #234234

    Purpose

    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pAIP4_5HIH

    Plasmid

    #234205

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pAIP4_2H2Z

    Plasmid

    #234147

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. paGFP

    Plasmid

    #18697

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    photoactivatable GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    CMV

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MS-1 magA

    Plasmid

    #21751

    Depositor

    Elliot Meyerowitz

    Article

    Bertani et al Gene. 2001 Feb 21. 264(2):257-63.

    Insert

    magA (magA M. magnetotacticum)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pPS904

    Plasmid

    #8850

    Depositor

    Pamela Silver

    Article

    Kahana et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

    Insert

    NUF2-sGFP (NUF2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

27. SARS2_HisSUMOTEV-nsp7/nsp8

    Plasmid

    #234405

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Inserts

    Nsp7_SARS2
    Nsp8_SARS2
    

    Use

    Tags

    His6-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NSP12:4393-5324_Nbio_Chis_BV

    Plasmid

    #234377

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp12_SARS2
    

    Use

    Baculovirus expresssion

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NSP13:5325-5925_BVnAvi

    Plasmid

    #234375

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp13_SARS2
    

    Use

    Baculovirus expresssion

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SARS1_NSP13_BV_NBIO-TEV_CTHROM-his

    Plasmid

    #234382

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp13_SARS
    

    Use

    Baculovirus expresssion

    Tags

    LVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)

    Plasmid

    #40999

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

    Type

    Empty backbone

    Use

    Tags

    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mCherry-Rhotekin(8-89)-mCherry-C1

    Plasmid

    #29675

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Nature. 2011 Mar 20. ():.

    Insert

    Rhotekin (Rtkn Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 8-89

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. NSP13:5325-5925_BV

    Plasmid

    #234374

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    Nsp13_SARS2
    

    Use

    Baculovirus expresssion

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EC67894

    Plasmid

    #209505

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU6

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EC67892

    Plasmid

    #209499

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGFP-HBx

    Plasmid

    #24931

    Depositor

    Xin Wang

    Article

    Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

    Insert

    HBx
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTK92_Lifeact-GFP

    Plasmid

    #46356

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    Lifeact
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    pBABE

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mGFP-10-sREACh-N3

    Plasmid

    #21947

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

    Insert

    mGFP-10-sREACh
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EC70133

    Plasmid

    #209507

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU6

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EC70134

    Plasmid

    #209508

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU6

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge