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mVenus
Plasmid#198192PurposeSpectral correction: Expression of mVenusDepositorInsertmVenus
UseTagsExpressionMammalianMutationA206KPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP
Plasmid#37825DepositorHas ServiceAAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1InsertGFP
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationN/APromoterCAGAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PprgH-GFPLVA
Plasmid#23340DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
Ecoli cpGFP
Plasmid#215713PurposeExpresses cpGFP in EcoliDepositorInsertcpGFP
UseTags6xHisExpressionBacterialMutationcircularly permuted GFPPromoterT7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6s
Plasmid#105714PurposeCan be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.DepositorHas ServiceAAV8InsertGCaMP6s
UseAAV; Flp/frtTagsExpressionMammalianMutationPromoterEf1a, Human elongation factor-1 alphaAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAquaN1
Plasmid#42888DepositorInsertAquamarine
UseTagsExpressionMammalianMutationT66S and H149GPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mhYFP
Plasmid#186524PurposeLocalization of monomeric hyperfolder YFP fluorescent protein to actinDepositorInsertMonomeric hyperfolder YFP
UseTagsLifeActExpressionMammalianMutationhfYFP-S147P/L195M/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pPROEX Aqua
Plasmid#42889DepositorInsertAquamarine
UseTags6xHis and TEV cleavageExpressionBacterialMutationT65S and H148GPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Flex-Axon-GCaMP7b
Plasmid#135419PurposeCan be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.DepositorInsertaxon-jGCaMP7b
UseAAVTags6xHis and GAP43 palmitoylation domainExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
VV073: 1xCox8 - GCaMP5G in fubi
Plasmid#58509PurposeExpresses the calcium sensor GCaMP5G in the mitochondriaDepositorInsert1xCox8 - GCaMP5G
UseLentiviralTagsExpressionMammalianMutationPromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-C1
Plasmid#22779DepositorInsertCyPet
UseTagsExpressionMammalianMutationControl for intermolecular Rac1 biosensor FRET. S…PromoterAvailable sinceDec. 18, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP-PEST
Plasmid#138658Purposedestabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yEGFP3-PEST
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJN105-Hygro-GFP
Plasmid#41883DepositorInsertPtac::GFP-mut-2
UseBacterial broad host range vectorTagsExpressionBacterialMutationGFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser…PromoterPtacAvailable sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP6fu
Plasmid#86235PurposeExpress fluorescent calcium indicator GCaMP6fu in mammalian cellsDepositorInsertGCaMP6fu
UseTagsExpressionMammalianMutationW43Y D395APromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-LSSmGFP
Plasmid#186534PurposeLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actinDepositorInsertLSSmGFP
UseTagsLifeActExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s
Plasmid#105715PurposeIn the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.DepositorHas ServiceAAV5Insertsynaptophysin-GCaMP6s
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1a, Human elongation factor-1 alphaAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-nlsGFP-W
Plasmid#126688PurposeConstitutive, ubiquitous, nuclear-localized GFP expression.DepositorInsertnlsGFP
UseLentiviralTags6xHIS and NLSExpressionMutationPromoterEF1aLAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYpet-C1
Plasmid#22780DepositorInsertYpet
UseTagsExpressionMammalianMutationControl for intermolecular Rac1 biosensor FRET. S…PromoterAvailable sinceDec. 18, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PssaG-GFPLVA
Plasmid#23343DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMA4854
Plasmid#184849PurposeCoexpression of PhiC31o recombinase and EGFPDepositorInsertsEGFP
PhiC31o
UseTagsExpressionMammalianMutationPromoterCMV and RSVAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD016
Plasmid#207932PurposePremade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
EB3-3xmTq2-N1
Plasmid#112929PurposeIn vivo visualization of microtuble plus ends (can be used for colocalization studies)DepositorArticleInsertEB3
UseTags3x mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdx1-mTQ2-P2A-FlpO-PA
Plasmid#67279PurposePancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertsmTQ2=mturquoise2 blue fluorescent gene
FlpO recombinase
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMammalianMutationPromoterpdxAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PfliC-GFPLVA
Plasmid#23342DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMZ2007
Plasmid#204399PurposeA 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsquorum sensing regulator LuxR
GFP
UseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PLux0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PsopB-GFPLVA
Plasmid#23341DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSW596 - mTurq2
Plasmid#115985Purposeentry vector for GreenGate cloning method, CDSDepositorInsertmTurquoise2
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Reciever_plasmid2
Plasmid#140695PurposeEncodes quorum sensing (C4-HSL) inducible cyan (sfCFP) expressionDepositorInsertsfCFP
UseTagsExpressionBacterialMutationPromoterengineered rhl promoter (responds to C4-HSL)Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PpipB-GFPLVA
Plasmid#23344DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGC247
Plasmid#19634DepositorInsertGateway(R1-R2)UseFlp/frt, destination vectorTags4xNLSExpressionWormMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGC245
Plasmid#19638DepositorInsertGateway(R4-R3)UseFlp/frt, destination vectorTags4xNLSExpressionWormMutationPromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
TOPO mT2
Plasmid#68491PurposeTOPO construct expressing mT2 for generation of GMAP-compatible geneDepositorInsertmTurquoise2
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGC97
Plasmid#19629DepositorInsertGateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)UseFlp/frt, destination vectorTagsExpressionWormMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGC238
Plasmid#19631DepositorInsertGateway(R1-R2)UseFlp/frt, destination vectorTags4xNLSExpressionWormMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2-EC1-G4S-mTQ2ΔC6
Plasmid#209091PurposemTurquoise2-inserted PcdhgB2DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKD013
Plasmid#207931PurposePremade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only
