We narrowed to 60,545 results for: tra

Showing: 721 - 740 of 60545 results

Results per page:

Sort results by:

Plk1 FRET sensor c-jun substrate

Plasmid

#45203

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Plk1 FRET sensor with c-jun substrate

Tags

CyPET, YPET

Expression

Mammalian

Promoter

T7

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-LRP4 (extracellular) [N207/27R]

Plasmid

#177513

Purpose

Mammalian Expression Plasmid of anti-LRP4 (extracellular) (Mouse). Derived from hybridoma N207/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-LRP4 (extracellular) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Gene Trap - 24XMS2 Lentiviral Vector

Plasmid

#174198

Purpose

Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

Depositor

Daniel Larson

Article

Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

Insert

iRFP

Use

Lentiviral

Tags

24X MS2 hairpins, Blasticidin, and T2A

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-CD69 CaRE3 (minus strand)

Plasmid

#91845

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0622)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (minus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd-Track Flag-SIRT1 H355A

Plasmid

#8439

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

Histidine 355 mutated to Alanine

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pET co-transformation cloning vector (13S-A)

Plasmid

#48323

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

Plasmid

#211817

Purpose

AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

N-terminus of SpCas9-ABE8e and tracrRNA

Use

AAV

Expression

Mammalian

Promoter

chicken β-actin promoter and U6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGal-Trans-Myc-eGFP-KDEL

Plasmid

#177729

Purpose

ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)

Tags

KDEL, Myc, and eGFP

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSaCas9-sgRNA-Tetra-com vector

Plasmid

#131227

Purpose

For expressing gRNA, whose Tetraloop is replaced with a com aptamer

Depositor

Baisong Lu

Article

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

vector for com modified sgRNA

Use

AAV

Expression

Mammalian

Available Since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

Plasmid

#133339

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-IL2RA CaRE4 (plus strand)

Plasmid

#91850

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0345)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

Plasmid

#197067

Purpose

Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

MitoTrap-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-IL2RA CaRE4 (minus strand)

Plasmid

#91849

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0626)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-3'UTR-Tetra-com-vector

Plasmid

#132553

Purpose

Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

Cas9
sgRNA scaffold with com replacing the Tetraloop

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-IL2RA CaRE1 (plus strand)

Plasmid

#91837

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0614)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE1 (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2-nbt-Electra1-P2A-mScarlet

Plasmid

#196113

Purpose

zebrafish expression of nbt-Electra1-P2A-mScarlet

Depositor

Reinhard Köster

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

nbt-Electra1-P2A-mScarlet

Use

Zebrafish expression

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
P-miR-145 (pTransluc-miR-145-1)

Plasmid

#21496

Depositor

Kenneth Kosik

Article

Xu et al Cell. 2009 May 15. 137(4):647-58.

Insert

1.5kb 5' of miR-145 (MIR145 Human)

Use

Luciferase

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

Plasmid

#133342

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-CD69 CaRE3 (plus strand)

Plasmid

#91844

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0621)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (plus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK-flag-GFP 1-10 tetra Cys

Plasmid

#78589

Purpose

Expression flag-GFP 1-10 tetra Cys in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 1-10 tetra Cys

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only