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Plasmid#227617PurposeOverexpression of SLC25A46 with R340C mutation in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
UseTagsP2A-mElectra1ExpressionMammalianMutationR340CPromoterCMV, T7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13)
Plasmid#227618PurposeOverexpression of SLC25A46 with I349D mutation in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
UseTagsP2A-mElectra1ExpressionMammalianMutationI349DPromoterCMV, T7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated
Plasmid#215769Purposeencodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locusDepositorInsertsUseTagsExpressionBacterialMutationCas12a PAM mutatedPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA
Plasmid#211819Purposelentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishmentDepositorInsertSpCas9, tracrRNA and blasticidin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterU1a promoter and miniU6 promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA
Plasmid#201964PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationE162A,M164A,R167APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA
Plasmid#201968PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationW255A,N262E,K266EPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-S13AI15AD30KT35A-V5His6_E
Plasmid#146219PurposeInsect Expression of DmTral-LSm-S13AI15AD30KT35ADepositorInsertDmTral-LSm-S13AI15AD30KT35A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R21A-V5His6_E
Plasmid#146221PurposeInsect Expression of DmTral-LSm-R21ADepositorInsertDmTral-LSm-R21A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-S13AI15AR21A-V5His6_E
Plasmid#146222PurposeInsect Expression of DmTral-LSm-S13AI15AR21ADepositorInsertDmTral-LSm-S13AI15AR21A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]
Plasmid#149427PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSmTex-del46-49-V5His6_B
Plasmid#145995PurposeInsect Expression of DmTral-LSmTex-del46-49DepositorInsertDmTral-LSmTex-del46-49 (tral Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-E23K-V5His6_B
Plasmid#145983PurposeInsect Expression of DmTral-LSm-E23KDepositorInsertDmTral-LSm-E23K (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21E-V5His6_B
Plasmid#145984PurposeInsect Expression of DmTral-LSm-R21EDepositorInsertDmTral-LSm-R21E (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSmTex-del46-49-V5His6_B
Plasmid#145985PurposeInsect Expression of DmTral-LSmTex-del46-49DepositorInsertDmTral-LSmTex-del46-49 (tral Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-E23K-V5His6_B
Plasmid#145990PurposeInsect Expression of DmTral-LSm-E23KDepositorInsertDmTral-LSm-E23K (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R21ER42EF44A-V5His6_B
Plasmid#145991PurposeInsect Expression of DmTral-LSm-R21ER42EF44ADepositorInsertDmTral-LSm-R21ER42EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R21ES13AI15A-V5His6_B
Plasmid#145992PurposeInsect Expression of DmTral-LSm-R21ES13AI15ADepositorInsertDmTral-LSm-R21ES13AI15A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R21E-V5His6_B
Plasmid#145993PurposeInsect Expression of DmTral-LSm-R21EDepositorInsertDmTral-LSm-R21E (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R42E-V5His6_B
Plasmid#145994PurposeInsect Expression of DmTral-LSm-R42EDepositorInsertDmTral-LSm-R42E (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23K-V5His6_C
Plasmid#146073PurposeInsect Expression of DmTral-LSm-R21EE23KDepositorInsertDmTral-LSm-R21EE23K (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only