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CoV2-Spike-EF1a-D614G-N501Y

Plasmid

#177939

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Expression

Mammalian

Mutation

D614G, N501Y

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1NTD-NLS

Plasmid

#174826

Purpose

Mammalian expression of human MLH1NTD-NLS (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1_NTD-NLS (codon optimized)

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

truncation of residues 1-335

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

Plasmid

#162378

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-ER-TurboID

Plasmid

#149415

Purpose

Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3A(1-10)

Plasmid

#157992

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

W148L, M150V, K153M, N194K, F204L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXA2

Plasmid

#153110

Purpose

Expression of Flag tagged human FOXA2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXA2 (FOXA2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPRx-Sth1Cas9-L5

Plasmid

#140993

Purpose

Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

Depositor

Wilbert Bitter

Article

Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

Inserts

Sth1Cas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified; Integrative in mycobactreia

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#126217

Purpose

Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b_Bst-LF

Plasmid

#153313

Purpose

Bacterial expression of the Bst Large Fragment

Depositor

Drew Endy, Philippa Marrack

Insert

Bst Large Fragment

Expression

Bacterial

Available Since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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SaABE8e

Plasmid

#138500

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSL0527 (pDonor)

Plasmid

#130634

Purpose

Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (Mini-Tn)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

Plasmid

#129405

Purpose

circular Pepper RNA

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-Pepper(TAR Variant-2)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pQE60-EFTu-His

Plasmid

#124127

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Tu (tufB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-FLAG-cGAS

Plasmid

#127661

Purpose

Over-express EGFP-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

EGFP-Flag

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TetOn-dCas9-KRAB

Plasmid

#115545

Purpose

Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Type

Empty backbone

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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FU-H2B-GFP-IRES-Puro

Plasmid

#69550

Purpose

Lentiviral, selectable fluorescent GFP tagging of nuclei.

Depositor

Charles Gersbach

Insert

Histone 2B - green fluorescent protein fusion

Use

Lentiviral

Tags

GFP fusion

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-8xHis-Ub

Plasmid

#107392

Purpose

Mammalian expression 8xHis-Ub

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

8 tandem repeats of 6xHis-Ub (See comments below)

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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SMARCA2-GFP

Plasmid

#65390

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA2 (SMARCA2 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p426MET25_sfpHluorin (MRV55)

Plasmid

#115697

Purpose

superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

Depositor

Eckhard Boles

Article

Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

Insert

sfpHluorin

Expression

Yeast

Mutation

for introduced mutations see publication

Promoter

MET25

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pR_LG lenti-(empty)-del-CTS

Plasmid

#112895

Purpose

Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries

Depositor

Paul Blainey

Article

Feldman et al bioRxiv 262121

Type

Empty backbone

Use

Lentiviral

Promoter

none

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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