162,408 results

1. TDP-43 mRuby

   Plasmid

   #216225

   Purpose

   TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGTag-gal4-VP16-B-actin

   Plasmid

   #117817

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   gal4/VP16
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWPXLd

   Plasmid

   #12258

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3EF1aH-myr-Akt

   Plasmid

   #179909

   Purpose

   This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

   Depositor

   Xin Chen

   Article

   Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

   Plasmid

   #158962

   Purpose

   Cre-dependent Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   hsp70l promoter
   floxed DsRed cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCmini-iCAP-PHP.V1

   Plasmid

   #127847

   Purpose

   non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.V1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF1037

   Plasmid

   #144269

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   WT1 (WT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSV Sport RXR alpha

   Plasmid

   #8882

   Depositor

   Bruce Spiegelman

   Article

   Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

   Insert

   RXR alpha (RXRA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. FH-Tet2-pEF

   Plasmid

   #41710

   Purpose

   This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

   Insert

   tet methylcytosine dioxygenase 2 (Tet2 Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST_FLAG-HUWE1

    Plasmid

    #187120

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pME tdTomato-CAAX

    Plasmid

    #135204

    Purpose

    Red fluorescent tag

    Depositor

    David Tobin

    Article

    Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

    Insert

    tdTomato
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GB1-A11(2-196, G38R)-Strep

    Plasmid

    #196209

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G38R

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS-lpp0.2-K64A

    Plasmid

    #201789

    Purpose

    mutant of PadR; transcriptional regulator can respond to p-coumaric acid

    Depositor

    Yajun Yan

    Article

    Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

    Insert

    PadR (padR Bacillus subtilis)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K64A

    Promoter

    Available since

    July 12, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 3xERRE/ERE-luciferase

    Plasmid

    #37852

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    3 copies of ERRE/ERE upstream of luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX-MCS-ASC-GFP

    Plasmid

    #73957

    Purpose

    Express human ASC with a C-terminal EGFP tag in mammalian cells

    Depositor

    Christian Stehlik

    Article

    de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

    Insert

    PYD and CARD domain containing, PYCARD (PYCARD Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-MH6-Bsu

    Plasmid

    #163911

    Purpose

    Expresses Bsu large fragment for affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    Bsu polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Flpe

    Plasmid

    #13787

    Purpose

    Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe-HAZ

    Plasmid

    #17383

    Depositor

    Stephen Goff

    Article

    Gao et al Science. 2002 Sep 6. 297(5587):1703-6.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-SUMO-hGSDMD

    Plasmid

    #111559

    Purpose

    Express human GSDMD in E.coli

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (GSDMD Human)
    

    Use

    Tags

    His, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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