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pRN111
Plasmid#84463PurposepJB38-NWMN29-30 + SarA_P1-mCherry-TermDepositorInsertSarA_P1-mCherry from pRN11
UseTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-his-BACH1 WT
Plasmid#17642DepositorInsertBACH1 (BRIP1 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1aGFP2AKAT7(E508Q)-W
Plasmid#159293PurposeLentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoterDepositorInsertKAT7 E508Q
UseLentiviralTagsExpressionMammalianMutationCRISPR sites mutated (gRNA ID. 5 and A10) and E50…Promoterhuman EF1a promoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJQ200SK
Plasmid#78497PurposeSuicide vector for gene replacementDepositorTypeEmpty backboneUseSuicide plasmidTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB
Plasmid#99391PurposeGateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)DepositorTypeEmpty backboneUseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabePuro-ApoECDS
Plasmid#42949DepositorInsertApolipoprotein E (APOE Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-pBAD
Plasmid#54553PurposeLocalization: Protein Expression Vector , Excitation: 462, Emission: 492DepositorTypeEmpty backboneUseTags6xHis and mTFP1ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE59
Plasmid#90404PurposeSTAT3 - Sis-inducible element (SIE) gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterIRF1Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-PM-TurboID-V5_IDG-K
Plasmid#214455PurposeGateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertPM-TurboID-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)
Plasmid#197502PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+36)
Plasmid#199165Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+36)
UseTagsMGHHHHHHGGAExpressionMutationE16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2
Plasmid#223164Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (WT)
Plasmid#42202DepositorInsertc-SRC (SRC Human)
UseTagsExpressionMammalianMutationL176F mutation compared to reference sequence NM_…PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKL06
Plasmid#104430Purposesuper Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertSuper Ecliptic pHluorin-mRuby2
UseTagsExpressionYeastMutationPromoterTef1Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP
Plasmid#183916PurposeReverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline additionDepositorInsertrTetR-GFP
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO SF-HERC2 (ShB-R)
Plasmid#55613PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cellsDepositorInsert3xFLAG tagged HERC2 (WT) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationSilent mutations have been incorporated into the …PromoterAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDAC439
Plasmid#195237PurposeMammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
1227 pcDNA3 Myr HA Akt2
Plasmid#9016DepositorInsertAkt2 (AKT2 Human)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla
Plasmid#60510PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only
