Addgene

Showing: 7401 - 7420 of 16452 results

Results per page:

Sort results by:

pGL3-Rb WT

Plasmid

#32390

Depositor

Julien Sage

Article

Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

Insert

Rb promoter (Rb1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
TAL2236

Plasmid

#36687

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CHD4-TALEN_Left (Chd4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
PIGAZFN-R2

Plasmid

#37800

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PIGA zinc finger R2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
phNFL-Q332P

Plasmid

#132599

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Human)

Use

Tags

Expression

Mammalian

Mutation

995A>C;Gln332Pro

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_omega2

Plasmid

#68239

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P01

Plasmid

#120012

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
C455-E01: attB4-stuffer-attB5

Plasmid

#162983

Purpose

Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C515-E01: attB5r-stuffer-attB1r

Plasmid

#162968

Purpose

Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBGSA FPR1

Plasmid

#62600

Purpose

Constitutive expression of FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Insert

FPR1 (FPR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only
pTol2-HybridBarcode

Plasmid

#174037

Purpose

For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

CRISPR target sites

Use

Tol2 transgenesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_Lyn11-STf

Plasmid

#182008

Purpose

CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lyn11-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKI-cjVASA-tdTomato

Plasmid

#160872

Purpose

Marmoset Targeting

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

Insert

tdTomato

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P32

Plasmid

#120043

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PIGAZFN-L2

Plasmid

#37798

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

PIGA zinc finger L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Use

Tags

myc eGFP 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z12

Plasmid

#120055

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-rhodopsin-CTS

Plasmid

#86071

Purpose

A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Rhodopsin (RHO Bovine)

Use

Tags

GST

Expression

Bacterial

Mutation

contains the region from 309-348 of Rhodopsin

Promoter

tac

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z25

Plasmid

#120068

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KG#470

Plasmid

#110930

Purpose

Expresses proteins pan-neuronally in C. elegans (entire nervous system)

Depositor

Kenneth Miller

Article

Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

Inserts

rab-3 promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_TRE_sg83-PDGFR_EF1a_iRFP670

Plasmid

#186311

Purpose

Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg83-PDGFR
iRFP670

Use

Tags

His, myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge