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2a-copGFP(+HA)

Plasmid

#83806

Purpose

Express copGFP reporter for HDR-induced knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

copGFP

Use

Homologous recombination for gapdh loci

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGY53

Plasmid

#221709

Purpose

Empty Cas12a vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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scFv-IFP2.0N

Plasmid

#215746

Purpose

Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0N

Expression

Mammalian

Promoter

SFFV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pNES-VL12.3

Plasmid

#43862

Depositor

Dane Wittrup

Article

Colby et al Proc Natl Acad Sci U S A. 2004 Dec 21;10

Insert

VL12.3

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
GFP-DDK

Plasmid

#137764

Purpose

Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

DDK

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCK013

Plasmid

#105345

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK016

Plasmid

#105348

Purpose

Bacterial translocation for proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK014

Plasmid

#105346

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pUC Ad VAI

Plasmid

#175518

Purpose

For mammalian expression of adenovirus VAI RNA

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

Ad VAI RNA

Use

Adenoviral

Promoter

lac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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CP1249-ABEmax

Plasmid

#119809

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1249)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Tom7

Plasmid

#66571

Purpose

Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae

Depositor

Orion Weiner

Article

Jost et al ACS Synth Biol. 2015 Jun 2.

Insert

PhyB (PHYB Mustard Weed)

Tags

Tom7 and mCherry

Expression

Yeast

Mutation

PhyB portion contains aa's 1-908

Promoter

pADH1

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd1

Plasmid

#42253

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 1 (Fzd1 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
Histone H2B targeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45216

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

Histone H2B-targeted Aurora B FRET sensor

Expression

Mammalian

Promoter

T7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pJEP205-AAV-TRE3G-GFP-pA

Plasmid

#164789

Purpose

AAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR(T/A)

Plasmid

#140052

Purpose

Negative control mutant for ABKAR biosensor.

Depositor

Jin Zhang

Article

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCK019

Plasmid

#105378

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor EXP7 (At1g12560; 750bp)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ar1

Plasmid

#225219

Purpose

Expression androgen receptor 1 in mammalian cells

Depositor

Deshou Wang

Article

Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

Insert

androgen receptor 1

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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LAMP1-ABKAR

Plasmid

#65068

Purpose

Expression of ABKAR biosensor targeted to the lysozome

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

ABKAR biosensor

Tags

Lamp1

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
pHAGE-UBC-MCP-mNeonGreen

Plasmid

#207581

Purpose

The lentiviral plasmids for expression of MCP-NeonGreen

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

MS2 coat protein mNeonGreen fusion

Use

Lentiviral

Tags

mNeonGreen

Promoter

UBC

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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Nuc-ABKAR

Plasmid

#61511

Purpose

Expression of nuclear AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

NLS (see comments)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-hNoxo1

Plasmid

#58530

Purpose

expresses human Noxo1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase organizer 1 (NOXO1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1H3_Hormone-recep

Plasmid

#109950

Purpose

Protein expression and purification of NR1H3_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1H3_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Dicer1

Plasmid

#14779

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pELuc

Plasmid

#118056

Purpose

GBPart - ELuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

ELuc

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJBL727

Plasmid

#140397

Purpose

Expresses CsoR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-CsoR-T

Tags

6XHis-TEV

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pPM1001

Plasmid

#167548

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Inaba serotype

Use

Cosmid

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-NHERF1-PDZ1-2

Plasmid

#28294

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 1-298 (deletion of the EB domain aa 2…

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pLSSmOrange-alpha-Tubulin

Plasmid

#37137

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human alpha-tubulin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NucGFP

Plasmid

#184706

Purpose

Lentiviral transfer plasmid for expression of NucGFP

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

NucGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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cr3Ec

Plasmid

#184860

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, gam, bet, exo

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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