We narrowed to 10,337 results for: plasmids 101

1. B-evoSpCas9

   Plasmid

   #126765

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

   Plasmid

   #136651

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9:CNRZ_1066 Chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and hU6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMTgR2

   Plasmid

   #107286

   Purpose

   Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

   Depositor

   Bohdan Schneider

   Article

   Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

   Insert

   Interferon gamma receptor 2 (IFNGR2 Human)
   

   Use

   Tags

   CATCATCACCATCACCATGA

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein promoter

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE2-TetO-SOX17E57A

   Plasmid

   #216177

   Purpose

   Generate lentiviruses encoding for human SOX17E57A (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE2-TetO-SOX17E57C

   Plasmid

   #216178

   Purpose

   Generate lentiviruses encoding for human SOX17E57C (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE2-TetO-SOX17E57D

   Plasmid

   #216179

   Purpose

   Generate lentiviruses encoding for human SOX17E57D (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE2-TetO-SOX17E57G

   Plasmid

   #216181

   Purpose

   Generate lentiviruses encoding for human SOX17E57G (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE2-TetO-SOX17E57H

   Plasmid

   #216182

   Purpose

   Generate lentiviruses encoding for human SOX17E57H (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHAGE2-TetO-SOX17E57I

   Plasmid

   #216183

   Purpose

   Generate lentiviruses encoding for human SOX17E57I (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-DIO- alpha-MSH

    Plasmid

    #239044

    Purpose

    Overexpression of alpha-MSH

    Depositor

    Qingchun Tong

    Article

    Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

    Insert

    alpha-MSH (Pomc Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-DIO-beta-Endorphin

    Plasmid

    #239043

    Purpose

    Overexpression of beta endorphin

    Depositor

    Qingchun Tong

    Article

    Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

    Insert

    beta-Endorphin (Pomc Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-DIO-mPOMC

    Plasmid

    #239042

    Purpose

    Overexpression of POMC gene

    Depositor

    Qingchun Tong

    Article

    Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

    Insert

    mPOMC (Pomc Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV PHP.eB-CAG-Venus-P2A-Tau-RING

    Plasmid

    #233696

    Purpose

    AAV expression of Venus-P2A-Tau-RING from CAG promoter

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Venus-P2A-Tau-RING
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV 9P31-hSyn-Venus-P2A-Tau-RING

    Plasmid

    #233693

    Purpose

    AAV expression of Venus-P2A-Tau-RING from hSyn promoter

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Venus-P2A-Tau-RING
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXJ-Nluc-_(PLRSKK)-CA1-19

    Plasmid

    #232657

    Purpose

    Nluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assay

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CGA (CGA Human)
    

    Use

    Luciferase

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

    Plasmid

    #229823

    Purpose

    A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

    Plasmid

    #229837

    Purpose

    A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

    Plasmid

    #229840

    Purpose

    A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBiFC-SS-VC155-IRE1-dLD

    Plasmid

    #217751

    Purpose

    Expresses the VC fragment of BiFC-IRE1-dLD

    Depositor

    Christina Chan

    Article

    Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

    Insert

    VC155-IRE1-dLD (ERN1 Human)
    

    Use

    Tags

    Venus (155-238, A206K)

    Expression

    Mammalian

    Mutation

    deleted amino acids 29-408

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiCRISPRv2_sgALDH7A1

    Plasmid

    #218273

    Purpose

    CRISPR KO plasmid for ALDH7A1 in human cell lines

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    Inserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge