We narrowed to 12,722 results for: CAR

1. pRK5 Fyn deltaSH3

   Plasmid

   #16034

   Depositor

   Filippo Giancotti

   Article

   Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

   Insert

   c-Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Delta SH3: deletion of amino acids 76-141

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA

   Plasmid

   #108805

   Purpose

   Expresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa233
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA

   Plasmid

   #108806

   Purpose

   Expresses aa234 to C-terminus of PhiC31 integrase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa234 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-32a(+)-TGFB3m

   Plasmid

   #120289

   Purpose

   Produce human recombinant mTGFB3 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   monomeric Transforming Growth Factor-β3
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GAL4-Creb5 (1- 508)

   Plasmid

   #195030

   Purpose

   GAL4-Creb5 fusion construct (full length, 508aa)

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   GAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

   Plasmid

   #199567

   Purpose

   Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer

   Depositor

   Andrea Cimarelli, Yuxin Song

   Article

   Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

   Insert

   Trim69 isoform B (codon optimized) (TRIM69 Human)
   

   Use

   Retroviral; Allows for puromycin selection

   Tags

   Flag

   Expression

   Mutation

   codon optimized

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA

   Plasmid

   #108795

   Purpose

   Expresses N-terminus to aa571 of PhiC31 integrase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa571
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA

   Plasmid

   #108796

   Purpose

   Expresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment aa572 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FKBP and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC6A2

   Plasmid

   #131891

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Sijben et al Sci Rep. 2021 Jun 10;11(1):12290. doi: 1

   Insert

   SLC6A2 (SLC6A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGEX-6p-hDcp2 E147/8Q

    Plasmid

    #72217

    Purpose

    bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E147Q, E148Q and F404L (please see depositor'…

    Promoter

    tac

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pBp-FGFR2b-K660N

    Plasmid

    #45707

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K660N

    Promoter

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-HtrA2-EGFP S306A

    Plasmid

    #14122

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S306A

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

13. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid

    #86610

    Purpose

    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xEcoLeuT(CUA)_EF1_AnapRS

    Plasmid

    #174894

    Purpose

    AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AnapRS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. tet-pLKO shMKP5.v2 puro

    Plasmid

    #87793

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tet-pLKO shMKP5.v1 puro

    Plasmid

    #87792

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS1725

    Plasmid

    #105870

    Purpose

    High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    iCreERT2-FFrt-KanR-FFrt
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT2/CMV-eGFP.PB/PTK

    Plasmid

    #170540

    Purpose

    Expresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restored

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    Delta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCX4-cMyc

    Plasmid

    #36119

    Depositor

    Steven Dowdy

    Article

    Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid

    #86611

    Purpose

    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge