We narrowed to 20,464 results for: Cre

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CMV-LUC2CP/intron/ARE

Plasmid

#62858

Purpose

splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

Depositor

Gideon Dreyfuss

Article

Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

Insert

Luciferase

Use

Luciferase

Expression

Mammalian

Mutation

insertion of chimeric intron at nueotide poclsiti…

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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ADORA2B-DuET

Plasmid

#213175

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

ADORA2B (ADORA2B Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pOT_2 - lenti-EFS-tNGFR-2A-puro

Plasmid

#181971

Purpose

Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

tNGFR (NGFR Human)

Use

Lentiviral

Mutation

truncation: aa 11-276

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-M2-PPP1R12C S452A

Plasmid

#31679

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12C S452A (PPP1R12C Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Mutation

changed Serine 452 to Alanine

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only
ABCG1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176944

Purpose

Mammalian expression vector encoding ABCG1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ABCG1 (ABCG1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_FLI1_P2A_Hygro_Barcode

Plasmid

#120437

Purpose

Barcoded lentiviral vector to express FLI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FLI1 (FLI1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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sgRNA-Dual_filler(hU6_H1/7SK hybrid)-EF1as_Thy1.1_P2A_Neo

Plasmid

#239609

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and minimal H1/7SK hybrid promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1/7SKhybrid-filler

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM24

Plasmid

#65396

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM24 (TRIM24 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
Str-KDEL_SBP-EGFP-ColX

Plasmid

#222305

Purpose

Synchronize the trafficking of CollagenX from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CollagenX fused to SBP-EGFP (COL10A1 Human)

Expression

Mammalian

Mutation

signal peptide of COL10A1 removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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NKP66 pEGFP-N2 (1176)

Plasmid

#62039

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

MARCHV (MARCHF5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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EF1a_ERG_P2A_Hygro_Barcode

Plasmid

#120434

Purpose

Barcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ERG (ERG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ADRB3-DuET

Plasmid

#213182

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

ADRB3 (ADRB3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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TRE3G-ZIM3-Cas9-P2A-GFP-PGK-Blasti

Plasmid

#239604

Purpose

Doxycycline inducible CRISPRgenee construct with a blasticidin resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Promoter

TRE3G

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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RFP-ATF4

Plasmid

#125237

Purpose

ATF4 expression vector

Depositor

Rozita Yarmand

Article

Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

Insert

ATF4 (ATF4 Human)

Tags

RFP

Expression

Mammalian

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSL21-mCherry

Plasmid

#164410

Purpose

Retroviral expression plasmid of sgRNA with mCherry

Depositor

Junwei Shi

Article

Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

Plasmid

#164822

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Promoter

miniCMV and pGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/CD9-GFP

Plasmid

#104402

Purpose

Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

Depositor

Jens Gruber

Article

Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

Insert

CD9 (CD9 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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MCHR1-DuET

Plasmid

#213337

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

MCHR1 (MCHR1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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GNRHR-DuET

Plasmid

#213254

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GNRHR (GNRHR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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OPRD1-DuET

Plasmid

#213358

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OPRD1 (OPRD1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC1

Plasmid

#172994

Purpose

constitutive expression of FLAG-tagged WWC1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC1 (WWC1 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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GPR87-DuET

Plasmid

#213293

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GPR87 (GPR87 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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GPR55-DuET

Plasmid

#213285

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GPR55 (GPR55 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_puromycin

Plasmid

#65310

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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CNR2-DuET

Plasmid

#213214

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CNR2 (CNR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HTR2A-DuET

Plasmid

#213321

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

HTR2A (HTR2A Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_GATA6_P2A_Hygro_Barcode

Plasmid

#120445

Purpose

Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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piRFP670-N1-GalT

Plasmid

#87325

Purpose

To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

Depositor

Lei Lu

Article

Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

Insert

1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)

Tags

iRFP670

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_GATA4_P2A_Hygro_Barcode

Plasmid

#120444

Purpose

Barcoded lentiviral vector to express GATA4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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