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BiFC3

Plasmid

#190048

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC4

Plasmid

#190049

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC2

Plasmid

#190047

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172 - TAA)

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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BiFC1

Plasmid

#190046

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172)

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC2

Plasmid

#22975

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC2 (CRTC2 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Expression

Mammalian

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymNeongreen-SpHis5

Plasmid

#125704

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GFP-Nanobody

Plasmid

#61838

Purpose

Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

GFP-Nanobody

Tags

GST

Expression

Bacterial

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pBTR(hCc)

Plasmid

#61026

Purpose

Expresses horse holocytochrome c in E. coli

Depositor

Gary Pielak

Article

Patel et al Protein Expr Purif. 2001 Jul;22(2):220-4

Insert

horse cytochrome c (CYCS Equus*)

Tags

None

Expression

Bacterial

Mutation

*see comments

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-E2-Crimson

Plasmid

#197196

Purpose

Expresses the protein of E2-Crimson in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

E2-Crimson

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRU41

Plasmid

#167663

Purpose

ENTRY vector for gRNA1 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU42

Plasmid

#167664

Purpose

ENTRY vector for gRNA2 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pH2B-TagRFP675-N1

Plasmid

#44279

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Tags

Histone 2B

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pGK415-rtetTAK8N, L131L

Plasmid

#165969

Purpose

Expresses rTetTA (K8N, L131L) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

tetR

Use

Synthetic Biology

Tags

three copies of VP16 (VP48)

Expression

Yeast

Promoter

pPGK1

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pM1s3AsG

Plasmid

#137921

Purpose

E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

Superfolder GFP

Use

Synthetic Biology; For use with e. coli nissle in…

Expression

Bacterial

Promoter

J23101

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EgI-HygroR

Plasmid

#207177

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pYS29

Plasmid

#202086

Purpose

Parent vector for producing universal minicircle donors for HaloTag N-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

NT HygR-T2A-Halo Minicircle Parent

Use

CRISPR

Tags

HaloTag

Promoter

None

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pQC-CherryOFF-GFP

Plasmid

#129101

Purpose

Reporter vector

Depositor

Fangliang Zhang

Article

Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

Insert

CherryOFF

Use

Retroviral

Expression

Mammalian

Mutation

G-> A

Promoter

CMV

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TrxRFP1

Plasmid

#98996

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRU43

Plasmid

#167665

Purpose

ENTRY vector for gRNA3 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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