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PB-CA-rtTA Adv

Plasmid

#20910

Purpose

piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

rtTA-ON-Adv

Use

piggybac

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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bZiPro

Plasmid

#86846

Purpose

express NS2B-NS3 protease in E.coli

Depositor

Dahai Luo

Article

Zhang et al Science. 2016 Dec 8. pii: aai9309.

Inserts

NS3 1-177
NS2B45-96

Tags

a hexahistidine tag and a thrombin cleavage site

Expression

Bacterial

Mutation

NS2B cofactor region (residues 45–96) and NS3 pro…

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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PB-CA

Plasmid

#20960

Purpose

piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Gateway Destination Vector

Use

piggybac

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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TRE.IkBSR-Flag Pgk.Cre

Plasmid

#22504

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

IkBSR

Use

Cre/Lox and Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pM370

Plasmid

#173178

Purpose

(Empty Backbone) gRNA-12xMBS

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

gRNA-12xMBS

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pM159

Plasmid

#173215

Purpose

Expresses dCas9-sfGFP10

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

dCas9-sfGFP10M2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pB006

Plasmid

#182320

Purpose

It's an empty replication plasmid backbone in Bacteroides

Depositor

Lei Dai

Article

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNBU2_repA1

Plasmid

#211910

Purpose

Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

Depositor

Adam Arkin

Article

Huang et al Nat Commun. 2024 Aug 5;15(1):6618. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)

Plasmid

#70742

Purpose

1 color weak reporter used in study

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

sfGFP-ssrA

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pUBC_V5_MS2-mCherry

Plasmid

#68420

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

V5 and mCherry

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

human UbC

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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TALED_Left-ND1-1397C-AD

Plasmid

#183892

Purpose

Expresses TALED_Left-ND1-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERK-SPARK

Plasmid

#106921

Purpose

Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

ERK-SPARK

Expression

Mammalian

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Rac1

Plasmid

#180576

Purpose

ddRFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Rac1

Expression

Mammalian

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-ND1-1397N

Plasmid

#183898

Purpose

Expresses TALED_Right-ND1-1397N in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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TALED_Left-ND1-AD-E1347A

Plasmid

#183894

Purpose

Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CDK4KTR

Plasmid

#126680

Purpose

Fluorescent reporter for CDK4/6 activity

Depositor

Hee Won Yang

Article

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

RB cterm

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEvol-NnSULT1C1-cysDNCQ

Plasmid

#188983

Purpose

biosynthesize sulfotyrosine in E. coli.

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Inserts

NnSULT1C1
cysD
cysN
cysC
cysQ

Expression

Bacterial

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_TAF15

Plasmid

#26379

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

TAF15 (TAF15 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only