We narrowed to 21,065 results for: ACE
-
Plasmid#166773PurposeEncodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only
-
ER-CanlonicSF
Plasmid#178343PurposeTo explore endoplasmic reticulum lactate dynamicsDepositorInsertER-CanlonicSF
TagsER Retention Signal, ER Signal Exon 1, and ER Sig…ExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-synaptophysin-Lime
Plasmid#201016PurposeExpresses Lime in synaptic vesicle lumen by fusion to synaptophysinDepositorAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAnc80L65
Plasmid#68837PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for detailsDepositorInsertAncestral AAV Capsid Anc80L65
UseAAV and Synthetic BiologyExpressionMammalianPromoterRep2Available SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-LMNB1
Plasmid#207771PurposeDonor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorInsertLMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSC101-Donor
Plasmid#140630PurposeKanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.DepositorInsertLE, RE, KanR, temperature sensitive pSC101 origin of replication
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-GOLGA2
Plasmid#227325PurposeDonor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)DepositorInsertGOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold-LMNB1
Plasmid#227329PurposeDonor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)DepositorInsertLMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVA2RBS035
Plasmid#121051PurposeExpresses recombinant MVA pathway in Escherichia coliDepositorInsertsmvaE
mvaS
mvaK1
mvaK2
mvaD
idi
Available SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-tdTomato
Plasmid#199250PurposeFluorescent labelling of bacterial cells with tdTomatoDepositorInsertCodon-optimized tdTomato gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3
Plasmid#89975PurposeExpresses GFP-tagged ataxin-3DepositorAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK301
Plasmid#87767PurposeE. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacIDepositorInsertPrhaBAD-sfGFP
UseSynthetic BiologyTags6xHis TagExpressionBacterialPromoterPrhaBAD rhamnose-inducible promoter from E. coliAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2
Plasmid#89735PurposeExpresses wildtype mouse TET2 in mammalian cellsDepositorAvailable SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
NC3 pCDNA3.1 FLAG WTX (1-804)
Plasmid#36958DepositorAvailable SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EMTB-TurboID-V5-puro
Plasmid#190741PurposeA lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)DepositorInsertEMTB-TurboID-V5 (RPS14 EMTB is human ensconsin; TurboID is engineered BirA from E.coli, Human)
UseLentiviralTagsMycExpressionMammalianMutationMicrotubule binding-domain of ensconsin (amino ac…PromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA
Plasmid#190790Purpose[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.DepositorInsertsCas9
mazF-LVA
UseCRISPR and Synthetic BiologyTagsLVAExpressionBacterialMutationsilent mutations of BpiI-sites in original Cas9 g…PromoterPnrsB and Ptrc-[E*](104)Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mCherry
Plasmid#199247PurposeFluorescent labelling of bacterial cells with mCherryDepositorInsertCodon-optimized mCherry gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-DIO-VGLUT2:miniSOG
Plasmid#214200PurposeFor Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)DepositorInsertSlc17a6 tagged with miniSOG and hemagglutinin
UseAAV and Cre/LoxTagsminiSOG (tagged with HA) is fused to the first in…ExpressionMammalianAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
LacZ in pLX304
Plasmid#42560DepositorInsertLacZ
UseLentiviralTags2xV5PromoterCMVAvailable SinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GPHN-FingR-mGold2s-CCR5TC
Plasmid#231785PurposeVisualization of inhibitory post-synapse using GPHN-FingR-mGold2sDepositorInsertGPHN-FingR-mGold2s-CCR5TC
ExpressionMammalianPromoterActin promoter with CMV enhancerAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only