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pXD69m2-AphA

Plasmid

#191247

Purpose

E. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP

Plasmid

#162033

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNSA-FLUC

Plasmid

#98143

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, zeocin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFUSE-B_P2rP4

Plasmid

#97185

Purpose

For fusion gene cloning. Recombine Gene B into this Gateway Entry Vector

Depositor

Kenneth Scott

Article

Lu et al Cancer Res. 2017 May 16. pii: canres.274

Type

Empty backbone

Use

Gateway Cloning

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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PM-NM!TA

Plasmid

#48651

Purpose

Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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JAK1 gRNA (BRDN0001144778)

Plasmid

#76393

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK1 (JAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEMJ_042

Plasmid

#214061

Purpose

T7 inducible LanTERN (pRSET)

Depositor

Pamela Silver

Article

Jones et al ACS Synth Biol. 2024 Feb 20. doi: 10.102

Insert

LanTERN

Tags

6x His, T7 Tag, and Xpress(tm) Tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB34

Plasmid

#158152

Purpose

Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS1302

Plasmid

#29147

Purpose

Plasmid described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Type

Empty backbone

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSL5200 (pDonor_ISGst3)

Plasmid

#210875

Purpose

Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

ISGst3

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHelper_Ec1_V1_ampR

Plasmid

#205292

Purpose

Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

Depositor

Scott Saunders

Article

Saunders et al bioRxiv 2023

Insert

CspRecT / Bxb-1

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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MUNC18-2

Plasmid

#186687

Purpose

Expresses MUNC18-2 in insect cells

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

MUNC18-2 (Stxbp2 Rat)

Expression

Insect

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-23

Plasmid

#186686

Purpose

Expresses SNAP-23 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

SNAP23

Tags

GST and thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1067A1

Plasmid

#164847

Purpose

Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

4 gRNAs and 3xP3-eGFP

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1067K

Plasmid

#164848

Purpose

Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

4 gRNAs and 3xP3-tdTomato

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSPNprM-hp

Plasmid

#48120

Purpose

Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

signal peptide of NprM

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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Ad:2CMV_A2R2-N25Ctr

Plasmid

#122243

Purpose

Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

Depositor

Corey Neu

Article

Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

Inserts

C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
Actinin alpha 2 (Actn2 Mouse)

Use

Adenoviral

Tags

Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

Expression

Mammalian

Mutation

deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

Promoter

CMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-PFAS-FLAG

Plasmid

#168226

Purpose

Expresses human PFAS-3xFLAG fusion in mammalian cells

Depositor

Noboru Mizushima

Article

Mimura et al J Biol Chem. 2021 Jan-Jun;296:100780. do

Insert

PFAS (PFAS Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEY52

Plasmid

#191055

Purpose

odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

odr-1 (odr-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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