We narrowed to 8,325 results for: gnal
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Plasmid#129122Purposeexpresses H258A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PirA
Plasmid#128959Purposeexpresses Acinetobacter baumannii PirA in E. coliDepositorInsertferric enterobactin receptor PirA
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeES157A
Plasmid#129121Purposeexpresses S157A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3/gamma2SIL
Plasmid#121433PurposeGABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorUseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1/gamma2SIL
Plasmid#121432PurposeGABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorUseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFN-Tel-TAL/pMXs-neo
Plasmid#63591PurposeExpresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the neomycin resistance gene.DepositorInsert3xFVN-Tel-TAL
UseRetroviral and TALENTags3xFLAG-tag, V5-tag, and nuclear localization sign…ExpressionMammalianMutationPromoterLTRAvailable SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(471-707)(C478S and C699T)
Plasmid#52132Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470 and changed C478S and C6…PromoterAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(282-470)
Plasmid#52125Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(282-470) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-281 and 471-707PromoterAvailable SinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBG MKK3b(Glu, delete LXL)
Plasmid#50452Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
UseTagsGstExpressionMammalianMutationdeleted L25-I27, changed Serine 218 to Glutamic A…PromoterSV40Available SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-N71
Plasmid#48720PurposeBacterial expression of Lacritin protein with 70 AA removed from the N-terminusDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationDeleted first 71 amino acids of mature lacritin w…PromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-pLAC-N45
Plasmid#48718PurposeBacterial expression of Lacritin protein with 45 AA removed from N terminusDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationDeleted first 45 amino acids of mature lacritin w…PromoterAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES
Plasmid#138299PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKCsub(T/A)-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PM-RA-BlastR
Plasmid#211708PurposeLentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)DepositorInsertPM-targetted RA (Lck Synthetic)
UseLentiviralTagsRAExpressionMammalianMutationPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
eDrFGFR1
Plasmid#183994PurposeOptically controlled eDrFGFR1 expression in mammalian cellsDepositorInserteDrFGFR1 (FGFR1 Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-R-GECO1
Plasmid#46021PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable SinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET29b-rhMOG
Plasmid#225484PurposeExpress the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coliDepositorInsertSoluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
UseTags6xHis tagExpressionBacterialMutationdeleted amino acids 1-29 (signal peptide) and 150…PromoterT7Available SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RasAR-NES
Plasmid#205567PurposeGenetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.DepositorInsertRasAR-NES (RAF1 Human, Synthetic)
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntr_Myc-BirA-G3-ER
Plasmid#173814PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
UseGateway shuttle vectorTagsMycExpressionMutationPromoterAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GPRC5D-DuET
Plasmid#213298PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only