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KTK_038

Plasmid

#180560

Purpose

Entry vector containing L3S2P11 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P11 terminator

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Co-NF-kB

Plasmid

#180197

Purpose

Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kB

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-1224

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFBP-2_6m.mutant

Plasmid

#162842

Purpose

Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6m_RNA-device

Expression

Yeast

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0065

Plasmid

#177019

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

1-deoxy-D-xylulose 5-phosphate synthase

Use

Synthetic Biology

Mutation

transit peptide removed, amplified from cDNA, con…

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-SQSfl

Plasmid

#171010

Purpose

HiLITR protease with full-length SQS targeting information (ER)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-SQS(FL)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 AtU6-26:AsCas12a_DR (GB1442)

Plasmid

#160574

Purpose

AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtU6-26:AsCas12a_DR

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 LbCas12a (GB1439)

Plasmid

#160573

Purpose

Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

LbCas12a

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 AsCas12a (GB1438)

Plasmid

#160572

Purpose

Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AsCas12a

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-EGxxFP-PIGA

Plasmid

#153958

Purpose

Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 401-bp fragment from intron 5 and exon 6)

Use

A plasmid specific for egxxfp assay

Tags

N/A

Expression

Mammalian

Mutation

No mutation

Promoter

CAG promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-EGxxFP-CD55

Plasmid

#153959

Purpose

Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)

Use

A plasmid specific for egxxfp assay

Tags

N/A

Expression

Mammalian

Mutation

No mutation

Promoter

CAG promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgUroD v4

Plasmid

#158036

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

UroD sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre sgItgb5 v3

Plasmid

#158038

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Itgb5 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre sgRipk4 v3

Plasmid

#158035

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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eNS2B47NS3 S135A

Plasmid

#155318

Purpose

DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant

Depositor

Dahai Luo

Article

Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

Insert

DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor

Tags

His tag

Expression

Bacterial

Mutation

S135A

Promoter

M13

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Halo-Tev- HSPB7(zebrafish) C49S

Plasmid

#108914

Purpose

Express C49S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7

Tags

Halo-TEV

Expression

Mammalian

Mutation

Cysteine 49 to Serine

Promoter

SP6

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-XYLT2-sgRNA

Plasmid

#154862

Purpose

Lentiviral expression of Cas9 and sgRNA targeting XYLT2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

XYLT2 sgRNA (XYLT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPN455

Plasmid

#137872

Purpose

Expression of gRNA targeting POGZ for CRISPRa; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPN447

Plasmid

#137871

Purpose

Expression of gRNA targeting POGZ for CRISPRi; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_3K

Plasmid

#131205

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 K209/410/538R (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K209/410/538R

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.16

Plasmid

#125083

Purpose

Expression of ompR(1-140aa)-ccaR(130-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)140
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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