We narrowed to 71,484 results for: nin

Showing: 7541 - 7560 of 71484 results

Results per page:

Sort results by:

pHS1928

Plasmid

#205967

Purpose

JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH all proteins, with His-tagged Cas8

Tags

14xHis on Cas8

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-NRCH-ABE8.17-m+V106W

Plasmid

#198551

Purpose

Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-ABE8.17-m+V106W

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_RBMX-eA3A-UdgX-nCas9

Plasmid

#163546

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RNF6_pLX307

Plasmid

#98366

Purpose

Lentiviral expression of RNF6

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RNF6 (RNF6 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS031 - pCAGGS-3XFLAG-BORIS-eGFP-bpA

Plasmid

#156443

Purpose

For transient expression of mouse BORIS (CTCFL) tagged with eGFP

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse BORIS (Ctcfl Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
2XMyc-LRRK2-3XKD/Y1699C

Plasmid

#25367

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS

Plasmid

#107327

Purpose

Expresses SpCas9 fused to dNmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead NmCas9 (D16A, D5…

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-SSeCKs T751A

Plasmid

#107142

Purpose

full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs T751A (Akap12 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed threonine 751 to alanine

Promoter

gag

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
P14-pZE-SUMO-ObiH

Plasmid

#204629

Purpose

ColE1 ori, KanR, TetR, Tet promoter with a codon optimized obiH gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.

Depositor

Aditya Kunjapur

Article

Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:

Insert

His-SUMO-TEV-ObiH

Use

Synthetic Biology

Tags

His Tag; SUMO Tag; TEV Protease Site

Expression

Bacterial

Promoter

PLtetO-1 promoter

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-lyzC-mcherry-2a-CDK2

Plasmid

#121131

Purpose

neutrophil specific expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

dre-WT CDK2 (cdk2 Zebrafish)

Tags

mcherry-2a

Expression

Bacterial

Promoter

neutrophil specific lyzC

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p2xFLAGhYAP2-S127A

Plasmid

#17794

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
TRAPPC8_pLX307

Plasmid

#98375

Purpose

Lentiviral expression of TRAPPC8

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TRAPPC8 (TRAPPC8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_eA3A T31A-dCas9

Plasmid

#163537

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-dCas9

Use

CRISPR

Expression

Mammalian

Mutation

dCas9 (D10A, H840A)eA3A T31A

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDESTmycEIF2C2

Plasmid

#19872

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 2 (AGO2 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only
pHS1862

Plasmid

#205975

Purpose

FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1859

Plasmid

#205972

Purpose

FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Csy3/Cas7 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1860

Plasmid

#205973

Purpose

FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas6/Csy4 from JAGBLH010000220, Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDECKO_mCherry_non-targeting

Plasmid

#140109

Purpose

CRISPR-Cas9 library validation (negative control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-DIO-(STChRger2-TS-EYFP)

Plasmid

#129394

Purpose

Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

soma targeted ChRger2

Use

AAV and Cre/Lox

Tags

Kv2.1-TS-EYFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge