We narrowed to 2,359 results for: T2A

1. pSH-Csy4-T2A-SpRFN

   Plasmid

   #85754

   Purpose

   Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-dCas9
   

   Use

   CRISPR

   Tags

   Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SOX2 KO (T2A-H2B-EGFP)

   Plasmid

   #167969

   Purpose

   SOX2 CDS replacement with T2A-H2B-EGFP

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 2 (SOX2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF11-wmiRFP2-T2A-HO1

   Plasmid

   #179485

   Purpose

   Expresses miRFP2 and HO1in worm cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   wmIRFP2-T2A-HO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NO

   Promoter

   tag-168 promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRF1-GW mNeongreen-T2A-mCherry

   Plasmid

   #125698

   Purpose

   Produces equimolar expression of mNeongreen and mCherry

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   mNeongreen-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-hCas9-T2A-miRFP670

   Plasmid

   #187223

   Purpose

   Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Cas9
   

   Use

   Tags

   Inserted T2A-miRFP670 at 3' terminal of MCS.

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDRF1-GW ymNeongreen-T2A-mTurquoise2

   Plasmid

   #125701

   Purpose

   Produces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeongreen-T2A-mTurquoise
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIHEU_miniSOG2 T2A H3.3A-EGFP

   Plasmid

   #87411

   Purpose

   UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

   Insert

   mini singlet oxygen generator 2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRF1-GW mScarletI-T2A-mTurquoise2

   Plasmid

   #118430

   Purpose

   Produces equimolar expression of mScarletI and mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   mScarletI-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

   Plasmid

   #105982

   Purpose

   This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.

   Depositor

   Marcel Bruchez

   Article

   Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19

   Insert

   FLEX-FAPdL5-POST-T2A-dTomato.FLEX
   

   Use

   AAV and Cre/Lox

   Tags

   FAPdL5-POST, T2A-dTomato, and myc

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin 1

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAav-TP53-T2A-BirA*

    Plasmid

    #115652

    Purpose

    rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102

    Inserts

    TP53 (TP53 Human)
    TP53 (TP53 Human)
    T2A
    BirA*
    

    Use

    AAV

    Tags

    BirA* and T2A

    Expression

    Mutation

    Homology region 1 and Homology region 2

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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