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We narrowed to 2,115 results for: PORA

Showing: 1481 - 1520 of 2115 results

  2. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  3. pLuc-Optop38i-dsm

    Plasmid
    #89748
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    Optop38i dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89748-plasmid-map-sequence-id-192493

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  7. pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA

    Plasmid
    #154781
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    CRFR1 (Crhr1 Rat)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA …
    Promoter
    EF1
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154781-plasmid-map-sequence-id-310400

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  8. pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA

    Plasmid
    #154780
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    CRFR1 (Crhr1 Rat)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154780-plasmid-map-sequence-id-310401

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  11. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162679-plasmid-map-sequence-id-321456

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  15. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

    Plasmid
    #176144
    Purpose
    EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Gly60 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176144-plasmid-map-sequence-id-353152

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  16. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)

    Plasmid
    #176143
    Purpose
    EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Arg65 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176143-plasmid-map-sequence-id-353151

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  17. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

    Plasmid
    #176139
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176139-plasmid-map-sequence-id-353012

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  18. pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro

    Plasmid
    #176089
    Purpose
    EGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutations in Lys35 to Ala, Lys68 to Ala, and Lys7…
    Promoter
    CMV
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176089-plasmid-map-sequence-id-352910

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  19. pSLIK_hygro_3xflag_NRF2(RR)

    Plasmid
    #136535
    Purpose
    Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)
    Depositor
    Kevin Janes
    Article
    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13
    Insert
    NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    This plasmid is developed by mutating 3 base pair…
    Promoter
    Available since
    July 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    136535-plasmid-map-sequence-id-304676

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  23. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid
    #176141
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutation in Asp256 to Ala, a…
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176141-plasmid-map-sequence-id-353041

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  24. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

    Plasmid
    #176140
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutations Lys35 to Ala, Lys6…
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176140-plasmid-map-sequence-id-353035

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  27. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Article
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Tags
    Expression
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155297-plasmid-map-sequence-id-307858

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  28. pOpen-THEAQlig

    Plasmid
    #165535
    Purpose
    Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    Taq DNA Ligase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165535-plasmid-map-sequence-id-339125

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  29. pOpen-9N7polA (CT)

    Plasmid
    #165502
    Purpose
    9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    9 degrees N-7 DNA Polymerase chain terminating
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165502-plasmid-map-sequence-id-338438

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  30. DONOR plasmid

    Plasmid
    #236552
    Purpose
    Contains the insert sequence IRES-HaloTag-T2A-PuroR flanked by microhomologies for incorporation downstream of HSPA5 (BiP/GRP78).
    Depositor
    Pablo Rivera-Fuentes
    Insert
    HaloTag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236552-plasmid-map-sequence-id-469275

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  31. LgBiT-LentiB3 Transfer Vector

    Plasmid
    #236912
    Purpose
    Lentiviral packaging plasmid for generation of LgBiT lentiviral particles to transduce HiBiT cell lines
    Depositor
    Promega Corporation
    Has Service
    DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    LgBiT
    Expression
    Mutation
    None
    Promoter
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236912-plasmid-map-sequence-id-474760

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  32. pEIAV-SIN6.1 CBGFPW

    Plasmid
    #44173
    Depositor
    John Olsen
    Insert
    enhanced green fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    chromophore mutations
    Promoter
    CB, CMV enhancer/chicken beta-actin promoter
    Available since
    May 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    44173-plasmid-map-sequence-id-59865

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  33. pGloSensor -40F cGMP Vector

    Plasmid
    #236812
    Purpose
    Express -40F cGMP in biosensor assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    -40F
    Use
    Luciferase
    Tags
    Expression
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236812-plasmid-map-sequence-id-471819

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  34. pGloSensor -42F cGMP Vector

    Plasmid
    #236806
    Purpose
    Express -42F cGMP in biosensor assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    -42F
    Use
    Luciferase
    Tags
    Expression
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236806-plasmid-map-sequence-id-471815

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  35. pPD1101 Integration Test Circuit Vector

    Plasmid
    #139251
    Purpose
    Integration vector with TUs incorporated. Cells will be red and, if treated with doxycycline, blue.
    Depositor
    Joshua Leonard
    Insert
    Tus
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139251-plasmid-map-sequence-id-337940

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  36. XLone-Puro eGFP

    Plasmid
    #140027
    Purpose
    Tunable and temporal expression control of nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140027-plasmid-map-sequence-id-275840

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  37. CMV HaloTag-LgBiT Vector

    Plasmid
    #236917
    Purpose
    Mammalian expression vector encoding HaloTag(R)-LgBiT for use in HiBiT cell lines
    Depositor
    Promega Corporation
    Has Service
    DNA
    Type
    Empty backbone
    Use
    Tags
    HaloTag (R)-LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236917-plasmid-map-sequence-id-474757

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  38. pNL [NlucP/ARE/Hygro] Vector

    Plasmid
    #236818
    Purpose
    Express ARE response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    ARE
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Expression
    Mutation
    None
    Promoter
    minP
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236818-plasmid-map-sequence-id-472870

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  39. pNL [NlucP/ISRE/Hygro] Vector

    Plasmid
    #236834
    Purpose
    Express ISRE response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    ISRE
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Expression
    Mutation
    None
    Promoter
    minP
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236834-plasmid-map-sequence-id-471835

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  40. pT7TS-rich

    Plasmid
    #99050
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Unspecified; Xenopus oocyte system
    Tags
    Expression
    Mutation
    Promoter
    pT7
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    99050-plasmid-map-sequence-id-191760

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Showing: 1481 - 1520 of 2115 results