We narrowed to 5,034 results for: plasmid dna

1. pSpCas9_T2A_iRFP670 sgBFP

   Plasmid

   #192478

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpCas9_T2A_mCherry sgBFP

   Plasmid

   #192929

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-SYN-PIK3CD

   Plasmid

   #203730

   Purpose

   AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Insert

   PIK3CD (PIK3CD Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human synapsin promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. POC1343

   Plasmid

   #221568

   Purpose

   Plasmid containing the Insert 1 to be assembled in POC1355

   Depositor

   Christopher A. O'Callaghan

   Insert

   DNA fragment flanked by BsaI sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. POC1344

   Plasmid

   #221569

   Purpose

   Plasmid containing the Insert 2 to be assembled in POC1355

   Depositor

   Christopher A. O'Callaghan

   Insert

   DNA fragment flanked by BsaI sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. POC1345

   Plasmid

   #221570

   Purpose

   Plasmid containing the Insert 3 to be assembled in POC1355

   Depositor

   Christopher A. O'Callaghan

   Insert

   DNA fragment flanked by BsaI sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. POC1346

   Plasmid

   #221571

   Purpose

   Plasmid containing the Insert 4 to be assembled in POC1355

   Depositor

   Christopher A. O'Callaghan

   Insert

   DNA fragment flanked by BsaI sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCW57-MCS1-P2A-MCS2 (Neo)

   Plasmid

   #89180

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLSLGUS

   Plasmid

   #51517

   Purpose

   pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   GUS
   3kb filler sequence
   

   Use

   Plant t-dna plasmid

   Tags

   NLS and flag

   Expression

   Mutation

   Promoter

   virion-sense LIR and 2x35S

   Available since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLSLZ.D

    Plasmid

    #51496

    Purpose

    pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    Zif268:FokI
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAH121

    Plasmid

    #164901

    Purpose

    The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

    Insert

    phi21 Integrase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4092677

    Plasmid

    #153883

    Purpose

    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stop

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    fragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wt;handful of SNPs as watermarks

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pSG7084 pG46-46

    Plasmid

    #232621

    Purpose

    pG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).

    Depositor

    Stephan Gruber

    Type

    Empty backbone

    Use

    Precursor plasmid to generate modified dna circles

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-sCAG-eGFP-RAB11

    Plasmid

    #203799

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-SYN-TdTomato-RAB11

    Plasmid

    #203732

    Purpose

    AAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-SYN-eGFP-RAB11

    Plasmid

    #203731

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQL269

    Plasmid

    #59802

    Purpose

    The univector plasmid-fusion system (UPS) uses Cre– lox site-specific recombination to fuse a plasmid containing the gene of interest and host vectors containing regulatory information.

    Depositor

    Stephen Elledge

    Article

    Liu et al Curr Biol. 1998 Dec 3;8(24):1300-9.

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lambdaPR

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

18. AAV-sCAG-eGFP-ACAP1

    Plasmid

    #203800

    Purpose

    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    ACAP1 (ACAP1 Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUPD2

    Plasmid

    #68161

    Purpose

    Universal domesticator plasmid for level 0 parts

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLSLZ

    Plasmid

    #51495

    Purpose

    pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    Zif268:FokI
    3kb filler sequence
    

    Use

    Plant t-dna plasmid

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRL528

    Plasmid

    #58495

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al Methods Enzymol. 1988;167:747-54.

    Insert

    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGM190

    Plasmid

    #69614

    Purpose

    inducible gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

    Inserts

    aphII
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Streptomyces- e. coli shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAIP4_4IPC

    Plasmid

    #234229

    Purpose

    Heterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4IPC (RPA1 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. AAV-sCAG-eGFP-ACAP1 F280E

    Plasmid

    #203801

    Purpose

    AAV vector plasmid expressing human ACAP1 mutation F280E fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    ACAP1 (ACAP1 Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    F280E

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-SYN-eGFP-Kindlin 1

    Plasmid

    #203733

    Purpose

    AAV vector plasmid expressing mouse kindlin-1 fused to eGFP under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    Fermt1 (Fermt1 Mouse)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. POC1358

    Plasmid

    #236103

    Purpose

    Assembled plasmid containing four inserts (from POC1343, POC1344, POC1345 and POC1346) assembled in POC1355

    Depositor

    Christopher A. O'Callaghan

    Insert

    DNA fragment flanked by BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid

    #124536

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Inserts

    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TB:7xSMAD_RE:MiniP

    Plasmid

    #133879

    Purpose

    7 copies the SMAD DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies the SMAD DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hisSUMO-ZFP57

    Plasmid

    #102716

    Purpose

    Express DNA binding domain of human Zinc finger protein 57 in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    Zinc finger protein 57 (ZNF57 Human)
    

    Use

    Tags

    hisSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TB:2xP53_RE:MiniP

    Plasmid

    #133881

    Purpose

    2 copies of the p53 DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    2 copies of the p53 DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pspCas9-Klenow-ST2-com-vector

    Plasmid

    #176235

    Purpose

    Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pspCas9-POLI-ST2-com vector

    Plasmid

    #176234

    Purpose

    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJSM101

    Plasmid

    #48791

    Purpose

    NHEJ reporter plasmid for 5' and 3' cohesive ends

    Depositor

    Michael Glickman

    Article

    Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

    Insert

    split lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJSM103

    Plasmid

    #48872

    Purpose

    NHEJ reporter plasmid for blunt ends

    Depositor

    Michael Glickman

    Article

    Zhu et al Biochemistry. 2012 Dec 21;51(51):10147-5

    Insert

    split lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. POC1426

    Plasmid

    #221573

    Purpose

    Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Insert

    DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. POC1427

    Plasmid

    #221574

    Purpose

    Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Insert

    DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. POC1428

    Plasmid

    #221575

    Purpose

    Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Insert

    DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. POC1429

    Plasmid

    #221576

    Purpose

    Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Insert

    DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGBW-m4092709

    Plasmid

    #153859

    Purpose

    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nearly native encoding;handful SNPs as watermarks…

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pCETF

    Plasmid

    #19783

    Depositor

    Jumi Shin

    Article

    Chen et al Biotechniques. 2008 Sep . 45(3):295-304.

    Type

    Empty backbone

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge