We narrowed to 7,122 results for: ef1a
-
Plasmid#66790PurposeExpression of murine PDGFRbeta tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRbeta (Pdgfrb Mouse)
UseTagsC-term EGFPExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
MCP-NMS
Plasmid#210705PurposeThis plasmid express EF1a promoter driven MCP-NMS transactivation moduleDepositorInsertMCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCTIP2-N174
Plasmid#60858PurposeExpresses CTIP2 under the EF1a promoterDepositorInsertCTIP2 isoform b (Bcl11b Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry-hCdt1(1-100)ΔCy
Plasmid#193759PurposeExpresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)DepositorInsertmCherry-hCdt1(1-100)ΔCy (CDT1 Human)
UseLentiviralTagsExpressionMutationhuman Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…PromoterEF1aAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL8 lenti-D10Acas9-3xflag-blast
Plasmid#112134Purposelenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)DepositorInsertD10Acas9-3xflag-blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A mutant in Cas9PromoterEF1aAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-mTurquoise-MLC-IRES-neo
Plasmid#85145Purposelentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoiseDepositorInsertmTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
UseLentiviralTagsmTurquoiseExpressionMutationPromoterEF1aAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-GFPL10
Plasmid#98704PurposeCre-dependently express GFPL10 fusion using Introvert SwitchDepositorInsertGFPL10
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationL10: 2609 C->T to remove EcoRI sitePromoterEF1aAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
crTet in pSLQ8453
Plasmid#183965PurposeU6-crTet-EF1a-Puro-T2A-BFP-WPREDepositorInsertcrTet
UseLentiviralTagsEF1a-Puro-T2A-BFPExpressionMammalianMutationPromoterU6Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MCP-3x9aaTAD
Plasmid#210707PurposeThis Plasmid express EF1a promoter driven MCP-3x 9aa transactivation moduleDepositorInsertMCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-Flag-Snrnp40
Plasmid#134253PurposeLentivector encoding Flag-tagged Snrnp40DepositorInsertSnrp40 (Snrnp40 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-iRFP-STOP-ReNL
Plasmid#113965PurposePiggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)DepositorInsertsiRFP670
ReNL
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCMV - SV40-PolyA - SV40-PolyA and EF1AAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti NbALFA-miRFP680 hygro
Plasmid#193969Purposelentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, hygromycin resistanceDepositorInsertNbALFA-miRFP680
UseLentiviralTagsmiRFP680ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti NbALFA-miRFP680 puro
Plasmid#193972Purposelentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, puromycin resistanceDepositorInsertNbALFA-miRFP680
UseLentiviralTagsmiRFP680ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti NbALFA-mNeonGreen puro
Plasmid#193973Purposelentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, puromycin resistanceDepositorInsertNbALFA-mNeonGreen
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti NbALFA-mNeonGreen hygro
Plasmid#193974Purposelentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, hygromycin resistanceDepositorInsertNbALFA-mNeonGreen
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-P-TAG (pGS594)
Plasmid#160039PurposepLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsertCP450TMD-APEX2-3xHA
UseLentiviralTagsExpressionMutationPromoterEF1Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY0551-Luciferase RADARS
Plasmid#182538PurposeMammalian cell expression plasmid of Gaussia luciferase RADARS in a dual luciferase expression back bone with Cypridina luciferase as the control gene.DepositorInsertRADARS Luciferase
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-U6-GFP-WPRE
Plasmid#200030PurposepiggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAsDepositorTypeEmpty backboneUseTagsGFPExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti-dsRed
Plasmid#201999PurposeBased on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsdsRedExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TK4-EGFP
Plasmid#239047PurposePiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiationDepositorInsertsEGFP
puroR
UseTagsExpressionMammalianMutationPromoterCAG and EF1aAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV Rnf121-V5
Plasmid#175130PurposeLentiviral expression of mouse Rnf121-V5DepositorInsertRnf121 (Rnf121 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP
Plasmid#219828PurposeOverexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
lenti-RfxCas13d-Blast-BFP
Plasmid#196726PurposeSortable and selectable RfxCas13d.DepositorInsertRfxCas13d-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsSV40 NLSExpressionMutationPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti.Cas9.BFP.Blast
Plasmid#196714PurposeWT SpCas9 with BSD-TagBFP for selection and sortingDepositorInsertCas9-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV Nup62-V5
Plasmid#175116PurposeLentiviral expression of mouse Nup62-V5DepositorInsertNup62 (Nup62 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Efla_FLEX_APEX2-eGFPf
Plasmid#171939PurposeExpresses a membrane targeted (farnesylation) APEX2-eGFP fusion proteinDepositorInsertAPEX2-eGFPf
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV Tor1a-V5
Plasmid#175135PurposeLentiviral expression of Tor1a-MycDepositorInsertTor1a (Tor1a Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Mospd1
Plasmid#175132PurposeLentiviral expression of V5-tagged mouse Mospd1DepositorInsertMospd1 (Mospd1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tyw1-V5
Plasmid#175131PurposeLentiviral expression of mouse Tyw1-V5DepositorInsertTyw1 (Tyw1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiMPH v2 (Neo)
Plasmid#92065PurposeModified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).DepositorInsertMS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Bidirectional MYC 5'UTR Dual Fluorescence Reporter
Plasmid#229498PurposeThis is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.DepositorInsertMYC 5'Untranslated region + destabilized GFP
UseLentiviralTagsExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-PDE4D3-Cat
Plasmid#169128PurposeAAV plasmid containing a mKate2 and a truncated PDE4D3DepositorUseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationtruncated, Catalatic domain onlyPromoterEF1aAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-iSAM
Plasmid#211495PurposeAAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRaDepositorInsertsUniSAM insert
TET-ON
Neo Resistance
Insulator
UseCRISPR and Synthetic BiologyTagsT2A-mCherry TagExpressionMammalianMutationPromoterEF1a, NA, Splicing acceptor, and TREGV promoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC150-KEIMA-VAPA
Plasmid#212096PurposeFluorescent reporter for VAPA clearance to acidic lysosomesDepositorInsertVAPA (VAPA Human)
UsePiggybacTagsmKeimaExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo
Plasmid#222636PurposeEzrin activation reporterDepositorInsertmiRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-Flag-h-SNRNP40
Plasmid#134254PurposeLentivector encoding Flag-tagged human SNRNP40DepositorInsertSNRP40 (SNRNP40 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Target Zfp462-Klf4SE
Plasmid#127668PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
UseCRISPRTagsHAExpressionMammalianMutationPromoterEF1a and hU6Available sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hyg-Snrnp40-CRISPR-resistant
Plasmid#134251PurposeLentivector encoding CRISPR-resistant Snrnp40DepositorInsertSnrp40 (Snrnp40 Mouse)
UseLentiviralTagsExpressionMammalianMutationmutated coding sequence “gataactatgcgacgttgaa” to…PromoterEF1aAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Promoter Only Target
Plasmid#127669PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 115 and 117
UseCRISPRTagsHAExpressionMammalianMutationPromoterEF1a and hU6Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo
Plasmid#222637PurposeEzrin activation reporter, T567 phosphorylation deficientDepositorInsertmiRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)
UseLentiviralTagsmRuby3ExpressionMutationT567APromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmx1-V5
Plasmid#175138PurposeLentiviral expression of V5-tagged mouse Tmx1DepositorInsertTmx1 (Tmx1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro_hCOL4A4
Plasmid#198099Purposelentiviral expression of hCOL4A4 in mammalian cellsDepositorInsertCOL4A4 (COL4A4 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LncRNA overexpression EF1α-BbsI-SV40polyA_puroR
Plasmid#219826PurposeOverexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 29, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV V5-Nesprin-3
Plasmid#175136PurposeLentiviral expression of V5-tagged mouse Syne3DepositorInsertSyne3 (Syne3 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Sun2
Plasmid#175114PurposeLentiviral expression of V5-tagged mouse Sun2DepositorInsertSun2 (Sun2 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-AktAR2
Plasmid#125195PurposeSB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporterDepositorInsertAktAR2
UseTransposonTags6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem9b-V5
Plasmid#175152PurposeLentiviral expression of mouse Tmem9b-V5DepositorInsertTmem9b (Tmem9b Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Ndc1-V5
Plasmid#175115PurposeLentiviral expression of mouse Ndc1-V5DepositorInsertNdc1 (Ndc1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tor1a-Myc
Plasmid#175134PurposeLentiviral expression of Myc-tagged mouse Tor1aDepositorInsertTor1a (Tor1a Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Sun1
Plasmid#175113PurposeLentiviral expression of V5-tagged mouse Sun1DepositorInsertSun1 (Sun1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only