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pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)

Plasmid

#70736

Purpose

plasmid for building 3n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Available Since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pL-rpb1-D260N

Plasmid

#10985

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D260N (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 778 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D261N

Plasmid

#10986

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D261N (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 781 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-R320C

Plasmid

#10987

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-R320C (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 958 C to T in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-E1230K

Plasmid

#10990

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-E1230K (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 3688 G to A in the RPB1 ORF resulting in…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pLLK010

Plasmid

#245133

Purpose

Based on pAF259 but with bitluc expression driven by the C. difficile minimal slpA promoter.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

PslpAmin-bitlucopt

Expression

Bacterial

Promoter

PslpAmin

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLLK011

Plasmid

#245134

Purpose

Based on pAF259 but with bitluc expression driven by the C. difficile minimal slpA promoter with an additional 37bp upstream.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

PslpAmid-bitlucopt

Expression

Bacterial

Promoter

PslpAmid

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pLLK012

Plasmid

#245135

Purpose

Based on pAF259 but with bitluc expression driven by the C. difficile minimal slpA promoter with an additional 332bp upstream.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

PslpAlong-bitlucopt

Expression

Bacterial

Promoter

PslpAlong

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK081

Plasmid

#245144

Purpose

Knock-in plasmid for insertion of DNA sequences between CD0188 (pyrE) and CD0189 in the C. difficile R20291 genome.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Ptet-mreB2

Use

Bacterial knock-in

Promoter

Ptet

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [M1-E186]-muGFP (Sus scrofa)

Plasmid

#238218

Purpose

Bacterial expression vector of porcine CFAP36 [M1-E186] with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [1-186]-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [W10E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238219

Purpose

Bacterial expression vector of porcine CFAP36 W10E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [W10E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [S92E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238220

Purpose

Bacterial expression vector of porcine CFAP36 S92E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [S92E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [A123E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238221

Purpose

Bacterial expression vector of porcine CFAP36 A123E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [A123E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [S163E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238222

Purpose

Bacterial expression vector of porcine CFAP36 S163E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [S163E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P CFAP36 [S180E]-3C-muGFP-10His (Sus scrofa)

Plasmid

#238223

Purpose

Bacterial expression vector of porcine CFAP36 S180E mutant with C-terminal tags

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [S180E]-3C-muGFP-10His

Tags

-3C-muGFP-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P ARL3-3xFlag-CFAP36-10His (Sus scrofa)

Plasmid

#238224

Purpose

Bacterial expression vector of porcine ARL3-3xFlag-CFAP36 fusion with C-terminal His tag

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

ARL3-3xFlag-CFAP36 -10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET156P ARL3-3xFlag-CFAP36 [S163E]-10His (Sus scrofa)

Plasmid

#238225

Purpose

Bacterial expression vector of porcine ARL3-3xFlag-CFAP36 [S163E] fusion with C-terminal His tag

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

ARL3-3xFlag-CFAP36 [S163E]-10His

Expression

Bacterial

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBR25 CFAP36-3C-mStayGold-2Strep crBSDr (C. reinhardtii)

Plasmid

#238226

Purpose

CRISPR donor plasmid for C-terminal tagging of CFAP36 and insertion of Blasticidin resistance (C. reinhardtii)

Depositor

Alan Brown

Article

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36

Use

CRISPR; Donor

Tags

-3C-mStayGold-2Strep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WT 30mer

Plasmid

#231888

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 30mer

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 30mer

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_DBD

Plasmid

#231886

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 DBD

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 DBD

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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